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Small inverted repeats (small palindromes) on plasmids have been shown to mediate a recombinational rearrangement event in Escherichia coli leading to the formation of inverted dimers (giant palindromes). This recombinational rearrangement event is efficient and independent of RecA and RecBCD. In this report, we propose a cruciform-dumbbell model to explain the inverted dimer formation mediated by inverted repeats. In this model, the inverted repeats promote the formation of a DNA cruciform which is processed by an endonuclease into a linear DNA with two hairpin loops at its ends. Upon DNA replication, this linear dumbbell-like DNA is then converted to the inverted dimer. In support of this model, linear dumbbell DNA molecules with unidirectional origin of DNA replication (ColE1 ori ) have been constructed and shown to transform E.coli efficiently resulting in the formation of the inverted dimer. The ability of linear dumbbell DNA to transform E.coli suggests that the terminal loops may be important in bypassing the requirement of DNA supercoiling for initiation of replication of the ColE1 ori. 相似文献
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Bonato P. Heng M.S.S. Gonzalez-Cueto J. Leardini A. O''Connor J. Roy S.H. 《IEEE engineering in medicine and biology magazine》2001,20(6):133-143
We have demonstrated a technique to calculate the EMG instantaneous median frequency to assess muscle fatigue during a dynamic exercise commonly prescribed in patients with ACL deficiency. We used Cohen-Posch time-frequency representations to improve upon the variability of the instantaneous median frequency estimates derived using Cohen Class transformations. The technique was applied to surface EMG data recorded from the quadriceps and hamstring muscles of a control subject and a patient with ACL deficiency during a repetitive squat exercise. Instantaneous median frequency values were derived for the knee-extension phases of the exercise. Ensemble average and standard deviation of the instantaneous median frequency were computed for the portion of the cycle associated with the lowest variability of the mechanics 相似文献
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