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81.
When three dimensional measurements are conducted with fringe projection, the quality of the grating used for the generation of the fringes is important. It has a direct influence on the achievable depth resolution in a given measurement setup. In the past, Ronchi grating or gratings written in nematic liquid-crystal displays or in digital micromirror devices have been used. We report on the application of a reflective ferroelectric liquid-crystal-on-silicon display as the fringe-generating element in a setup based on a stereo microscope. With this device the depth resolution of measurements by use of phase-shifting algorithms can be significantly improved compared with the application of a Ronchi grating or a nematic liquid-crystal display. 相似文献
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An approach for the characterization of glycosylation sites and oligosaccharide heterogeneity in glycoproteins based on a combination of nonspecific proteolysis, deglycosylation, and matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FT MS) is described. Glycoproteins were digested with Pronase yielding primarily glycopeptides and amino acids. Nonglycosylated peptide fragments were susceptible to complete Pronase digestion to their constituent amino acids. Steric hindrance prohibited the digestion of the peptide moiety attached to the glycan. Glycopeptides were desalted and concentrated using solid-phase extraction and analyzed by MALDI MS. The oligosaccharides were also analyzed by MALDI MS after releasing the glycans from glycoproteins using PNGase F. The peptide moiety of the glycopeptides was identified by subtracting the masses of the glycans derived from PNGase F treatment from the masses of the glycopeptides. The experimental strategy was validated using glycoproteins with known oligosaccharide structures, ribonuclease B and chicken ovalbumin. This procedure was then used to determine the N-glycosylation sites and site heterogeneity of a glycoprotein whose glycosylation pattern was unknown, namely, the Xenopus laevis egg cortical granule lectin. This procedure is useful for determining protein site heterogeneity and structural heterogeneities of the oligosaccharide moiety of glycoproteins. 相似文献
84.
We investigated which structural components are responsible for maintaining interstitial fluid equilibrium in the pulpal tissue, for which the existence of an effective lymph drainage is postulated. There have been only a small number of investigations on pulpal lymph tissue. Therefore, we decided to perform a detailed structural analysis. Twenty vital, healthy teeth that had to be extracted for orthodontic reasons were immersed in Patent Blue for 10 to 15 minutes after opening the pulpal cavity. They were then extracted and the dental pulps were opened by cleavage of the surrounding hard tooth structure. Subsequently, the specimens were prepared for light and electron microscopic investigation. A clear blue ring of stain was detected by light microscopy in Weil's zone in the coronal region of the pulp, the cell-rarefied layer surrounded by the odontoblasts. No dye deposition was observed in the apical part. However, using transmission electron microscopy, capillary structures with typical morphological characteristics of lymphatic vessels were found apically. The coronal part of the pulp did not reveal any such vascular structures. It may be concluded from these findings that the lymph in the coronal region is collected in interstitial tissue clefts and drained towards the apex, whence it is further transported via lymph capillaries. 相似文献
85.
Davis RH Zhao Y Galvin KP Wilson HJ 《Philosophical transactions. Series A, Mathematical, physical, and engineering sciences》2003,361(1806):871-894
Solid-solid contacts due to microscopic surface roughness in viscous fluids were examined by observing the translational and rotational behaviours of a suspended sphere falling past a lighter sphere or down an inclined surface. In both cases, a roll-slip behaviour was observed, with the gravitational forces balanced by not only hydrodynamic forces but also normal and tangential solid-solid contact forces. Moreover, the nominal separation between the surfaces due to microscopic surface roughness elements is not constant but instead varies due to multiple roughness scales. By inverting the system, so that the heavy sphere fell away from the lighter sphere or the plane, it was found that the average nominal separation increases with increasing angle of inclination of the plane or the surface of the lighter sphere from horizontal; the larger asperities lift the sphere up from the opposing surface and then gravity at large angles of inclination is too weak to pull the sphere back down to the opposing surface before another large asperity is encountered. The existence of microscopic surface roughness and solid-solid contacts is shown to modify the rheological properties of suspensions. For example, the presence of compressive, but not tensile, contact forces removes the reversibility of sphere-sphere interactions and breaks the symmetry of the particle trajectories. As a result, suspensions of rough spheres exhibit normal stress differences that are absent for smooth spheres. For the conditions studied, surface roughness reduces the effective viscosity of a suspension by limiting the lubrication resistance during near-contact motion, and it also modifies the suspension microstructure and hydrodynamic diffusivity. 相似文献
86.
Photomodification of polymer microchannels induced by static and dynamic excimer ablation: effect on the electroosmotic flow 总被引:2,自引:0,他引:2
This paper presents a study of polymer surfaces modified by laser ablation using poly(ethylene terephthalate) (PET) as a model system. The surface properties induced by static and dynamic ablation with the 193-nm pulsed radiation of an ArF excimer laser (4 x 10(7) W/cm2) in air have been successfully used to control the electroosmotic flow (EOF) in photoablated PET microchannels. Through the creation of well-defined static ablation patterns onto the walls of a trapezoidal channel, it was found that the resulting reduction in the EOF could be controlled. For example, a reduction of 25% in the EOF was observed in 42-microm-deep microchannels when using a static ablation pattern treating 50% of the total wall surface area. A numerical study describing the fluidic behavior induced by a static pattern is also presented. Moreover, X-ray photoelectron spectroscopy has been used to point out surface changes between static and dynamic ablation, thereby demonstrating an ability to create new functionalities in microchannels by laser treatment. 相似文献
87.
Highly Sensitive Pulsed Digital Holography for Built-in Defect Analysis with a Laser Excitation 总被引:7,自引:0,他引:7
A highly sensitive method is presented for noninvasive defect analysis on thin structures with a Q-switched double-pulsed ruby laser with frequency doubling (347 nm). In our research we feature an all-optical arrangement, where a focused laser pulse derived from the same ruby laser (694 nm) acts as a built-in synchronous excitation source for digital holographic interferometry. The recordings are made with a CCD camera for capturing two holograms (two states of the specimen) corresponding to the two UV laser pulses with a short time separation (10-50 mus). Subtraction of the phase distribution in two digital holograms gives a fringe phase map that shows the change in deformation of the specimen between the recordings. The advantage of the proposed method is two fold. First, the use of a shorter wavelength results in a higher sensitivity. Second, owing to the induced synchronous built-in optical excitation, the specimen is not subjected to any external physical excitation devices. Experimental results are presented on identification and evaluation of defects in thin metal sheets. 相似文献
88.
89.
90.
Misselwitz B Schmitt-Willich H Ebert W Frenzel T Weinmann HJ 《Magma (New York, N.Y.)》2001,12(2-3):128-134
Rationale and objectives: Gadomer-17 is a new magnetic resonance (MR) contrast medium presently in clinical development. It
is a dendritic gadolinium (Gd) chelate carrying 24 Gd ions. This study investigated the pharmacokinetic behavior of this contrast
medium. Methods: The pharmacokinetics of Gadomer-17 were investigated in different species (rat, rabbit, dog, monkey) for
up to 7 days after intravenous (i.v.) injection of 25–100 μmol/kg body weight. In addition, elimination and biodistribution
were evaluated after single i.v. injection of Gadomer-17 in rats. Results: After i.v. injection Gadomer-17 distributes almost
exclusively within the intravascular space without significant diffusion into the interstitial space. The volume of distribution
(Vc) in the initial or α-phase ranged from 0.04 1/kg (rats, rabbits) to 0.06 1 kg (monkeys) and 0.07 1/kg (dogs), which reflects
mainly the plasma volume. The blood/plasma concentration profile was found to be biphasic. The volume of distribution at a
steady state is clearly smaller than that of other contrast media, which distribute to the extracellular space. After single
i.v. injection in rats, the dendritic contrast medium was rapidly and completely eliminated from the body, mainly via glomerular
filtration. No long-term accumulation or retention of the nonmetabolized agent was detectable in organs or tissues. Conclusions:
Gadomer-17 is a promising new MR contrast medium that has an intravascular distribution and a rapid renal elimination. 相似文献