政府与大学关系的历史考察及启示

正确把握政府和大学的关系一方面有助于我们正确理解政府的高等教育政策，另一方面也有助于大学办学自主权的真正落实以及现代大学制度的逐渐形成，这对于我国高等教育发展而言无疑是具有现实意义的课题之一。本文在已有研究的基础上，转换研究视角，以政府与大学之间存在的利益关系为研究线索，发现并提出自大学产生以来政府与大学之间的四种关系形态：无共同利益期、国家利益主导期、大学利益主导期以及利益共生期。文中最后还对如何认识和理解当今我国政府与大学关系进行了讨论。     

基于故障录波回放的智能变电站保护测试技术研究

近年来,智能变电站进一步改良优化,继电保护智能化、标准化的思路应运而生.随着技术规范的不断完善和统一,智能变电站保护在测试方法和通信形式上较以往发生了很大改变,大量检测工作需要在工厂内进行,而继电保护常规检测方法无法满足工厂化大规模检测需求,这给智能变电站保护测试工作带来了新的挑战.针对上述问题,本文研究现有的智能变电站保护检测技术,分析采用故障录波文件回放的检测实验较常规动/静模检测实验的优点,设计了一套适用于不同录波装置的录波文件解析流程.此外,为了满足不同类型智能变电站保护装置采用故障录波文件回放进行检测的需求,研究了三种同步回放技术并分析其具体适用范围.     

Senescence Marker Protein 30 (SMP30): A Novel Pan-Species Diagnostic Marker for the Histopathological Diagnosis of Breast Cancer in Humans and Animals

Senescence marker protein 30 (SMP30) is a cell survival factor playing an important role in vitamin C synthesis and antiapoptosis. Moreover, its cytoprotective role suggests a possibility to be related to cancer cell survival. Mammary carcinoma is a common cancer in both humans and animals. Because of its histopathological diversity, especially in the early stage, histopathological diagnosis may be complicated; therefore, a diagnostic marker is helpful for confirmation. The present study analyzed the expression pattern of SMP30 in mammary carcinoma in humans, dogs, and cats. Immunohistochemistry, immunofluorescence, and western blot analysis were used to investigate SMP30 expression patterns. The expression was specifically observed in neoplastic glandular epithelial cells. The expression increased with the malignancy of glandular epithelial cells with a highly proliferative status. However, SMP30 expression was low in normal mammary gland tissues or well-differentiated adenoma tissues. The patterns were consistently reproduced in canine primary mammary carcinoma cells and MCF-7 and MDA-MB-231 human carcinoma cell lines. This study provides useful information to understand SMP30 expression in various stages of mammary carcinoma and to suggest its utility as a pan-species diagnostic marker, thereby helping to establish strategies for diagnosing mammary carcinoma in several species.     

Identification of Cyclophilin A as a Potential Anticancer Target of Novel Nargenicin A1 Analog in AGS Gastric Cancer Cells

We recently discovered a novel nargenicin A1 analog, 23-demethyl 8,13-deoxynargenicin (compound 9), with potential anti-cancer and anti-angiogenic activities against human gastric adenocarcinoma (AGS) cells. To identify the key molecular targets of compound 9, that are responsible for its biological activities, the changes in proteome expression in AGS cells following compound 9 treatment were analyzed using two-dimensional gel electrophoresis (2-DE), followed by MALDI/TOF/MS. Analyses using chemical proteomics and western blotting revealed that compound 9 treatment significantly suppressed the expression of cyclophilin A (CypA), a member of the immunophilin family. Furthermore, compound 9 downregulated CD147-mediated mitogen-activated protein kinase (MAPK) signaling pathway, including c-Jun N-terminal kinase (JNK) and extracellular signal-regulated protein kinase 1/2 (ERK1/2) by inhibiting the expression of CD147, the cellular receptor of CypA. Notably, the responses of AGS cells to CypA knockdown were significantly correlated with the anticancer and antiangiogenic effects of compound 9. CypA siRNAs reduced the expression of CD147 and phosphorylation of JNK and ERK1/2. In addition, the suppressive effects of CypA siRNAs on proliferation, migration, invasion, and angiogenesis induction of AGS cells were associated with G2/M cell cycle arrest, caspase-mediated apoptosis, inhibition of MMP-9 and MMP-2 expression, inactivation of PI3K/AKT/mTOR pathway, and inhibition of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) expression. The specific interaction between compound 9 and CypA was also confirmed using the drug affinity responsive target stability (DARTS) and cellular thermal shift assay (CETSA) approaches. Moreover, in silico docking analysis revealed that the structure of compound 9 was a good fit for the cyclosporin A binding cavity of CypA. Collectively, these findings provide a novel molecular basis for compound 9-mediated suppression of gastric cancer progression through the targeting of CypA.     

Biflavonoid-Induced Disruption of Hydrogen Bonds Leads to Amyloid-β Disaggregation

Deposition of amyloid β (Aβ) fibrils in the brain is a key pathologic hallmark of Alzheimer’s disease. A class of polyphenolic biflavonoids is known to have anti-amyloidogenic effects by inhibiting aggregation of Aβ and promoting disaggregation of Aβ fibrils. In the present study, we further sought to investigate the structural basis of the Aβ disaggregating activity of biflavonoids and their interactions at the atomic level. A thioflavin T (ThT) fluorescence assay revealed that amentoflavone-type biflavonoids promote disaggregation of Aβ fibrils with varying potency due to specific structural differences. The computational analysis herein provides the first atomistic details for the mechanism of Aβ disaggregation by biflavonoids. Molecular docking analysis showed that biflavonoids preferentially bind to the aromatic-rich, partially ordered N-termini of Aβ fibril via the π–π interactions. Moreover, docking scores correlate well with the ThT EC₅₀ values. Molecular dynamic simulations revealed that biflavonoids decrease the content of β-sheet in Aβ fibril in a structure-dependent manner. Hydrogen bond analysis further supported that the substitution of hydroxyl groups capable of hydrogen bond formation at two positions on the biflavonoid scaffold leads to significantly disaggregation of Aβ fibrils. Taken together, our data indicate that biflavonoids promote disaggregation of Aβ fibrils due to their ability to disrupt the fibril structure, suggesting biflavonoids as a lead class of compounds to develop a therapeutic agent for Alzheimer’s disease.     

BPDA/ODA体系微支化交联聚酰亚胺薄膜制备与性能研究

以三官能度的1,3,5-三(4-氨基苯氧基)苯(TAPOB)为交联剂,在3,3′,4,4′-联苯四甲酸二酐(BPDA)和4,4′-二氨基二苯醚(ODA)体系聚酰亚胺(PI)薄膜中构建微支化交联结构,制备出一系列具有不同TAPOB含量的PI薄膜,研究了TAPOB含量对薄膜力学性能、热力学性能、介电性能和吸水率的影响.结果表明:TA-POB的引入可明显提高BPDA/ODA体系PI薄膜的综合性能,交联结构的存在有利于提高薄膜的力学性能、降低热膨胀系数(CTE)和吸水率,微支化结构则对降低介电常数有一定的作用.     

LX—2型变频式喂线设备的研制及应用

根据炼钢生产的实际需要,分析了喂线技术的工艺要求,介绍了所研制的LX－2型变频式喂线机的结构特点,指出了影响喂线机使用效果的因素,并提出了若干措施。     

冶金工业的发展及其对冶金轧辊的需求

从冶金工业的发展现状可知高质量的轧辊仍是一个很大的市场。高速钢轧辊具有很强的竞争力。离心铸造法生产高速钢轧辊是主要的工艺方法。     

一种限制搜索区域的多比例尺最优路径规划算法*

针对现有大区域范围路径规划算法存在的一些问题,提出一种限制搜索区域的多比例尺最优路径规划算法。该算法在进行路径规划时,一方面根据路网的多比例尺信息对路网进行分级,另一方面对搜索区域进行合理限制。测试实验表明此算法可以提高路径规划的效率。     

The visual fiducial based pose estimation of mobile manipulator in large-scale components manufacturing

The accurate estimation of the end-effector’s pose in large operating spaces is the key for the mobile manipulator to realize efficient manufacturing of large and complex components. We propose a novel pose tracking method in large-range using visual fiducial markers, and further propose the layout optimization method for the encoded fiducial markers. A metric named orientational dilution of precision (ODOP) is proposed to evaluate the magnification of the pose estimation error compared with the measurement error of the coded fiducial markers. The distribution pattern of the coded markers is analyzed based on ODOP, and the square-shaped layout is determined to be a satisfactory distribution pattern for the minimum positioning unit of markers, and the side length of the square-shaped layout is further selected. The simulations and experiments prove the effectiveness of the ODOP index. Finally, the square-shaped layout and the designed distribution density for positioning coded markers are adopted to realize the high-precision measurement of large components by the mobile manipulator.