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901.
902.
将合链霉亲和亲-蛋白A(Streptavidin-proteinA)融合蛋白基因的表达质粒pTSAPA导入大肠杆菌BL21(DE3),成功地表达了该融合蛋白,经SIJS-PAGE及免疫印迹等方法证实该蛋白既具有IgG的结合活性,又具生物素结合活性。 相似文献
903.
Chien-Ming Wu Shu-Chun Wu Wan-Jung Chung Hsien-Cheng Lin Kun-Tze Chen Yu-Chian Chen Mei-Feng Hsu Jwu-Maw Yang Jih-Pyang Wang Chun-Nan Lin 《International journal of molecular sciences》2007,8(8):830-841
The known flavonoids ginkgetin (1), taiwanhomoflavone A (2), taiwanhomoflavone B (3), and taiwanhomoflavone C (4) and eight known lignans: justicidin B (9), justicidin C (10), justicidin D (11), chinensinaphthol methyl ether (12), procumphthalide A (13), procumbenoside A (15), and ciliatosides A (16) and B (17) were isolated from Cephalotaxus wilsoniana and Justicia species, respectively. The antiplatelet effects of the above constituents on human platelet-rich plasma (PRP) were evaluated. Of the compounds tested on human PRP, compounds 1, 4, 9, and 11 showed inhibition of secondary aggregation induced by adrenaline. Compound 1 had an inhibitory effect on cyclooxygenase-1 (COX-1). Molecular docking studies revealed that 1 and the related compounds apigenin (5), cycloheterophyllin (6), broussoflavone F (7), and quercetin (8) were docked near the gate of active site of COX-1. It indicated that the antiplatelet effect of 1, 4, 9, and 11 is partially owed to suppression of COX-1 activity and reduced thromboxane formation. Flavonoids, 1, 5, 6, 7, and 8 may block the gate of the active site of COX-1 and interfere the conversion of arachidonic acid to prostaglandin (PG) H2 in the COX-1 active site. 相似文献
904.
The mechanism of color reversion in soybean salad oil 总被引:5,自引:0,他引:5
Ming-Tain Lai Woan-Meei Lin Yan-Hwa Chu Shih-Ling Y. Chen Kwan-Shean Kong Chieh-Wu Chen 《Journal of the American Oil Chemists' Society》1989,66(4):565-571
An enzymatic reaction might be the major cause of color reversion in soybean oil. During the soybean flaking process, enzymatic
reactions take place to convertγ-tocopherol to 5-(tocopheryloxy)-γ-tocopherol (γ-TED), and then further convertγ-TED to the precursors which are the cause of color reversion. The variations ofγ-tocopherol andγ-TED contents in soybean oil are accompanied by an inverse change in the precursors’ concentration. Therefore, the contents
ofγ-tocopherol orγ-TED in crude soybean oil could be used as an index for predicting the color quality (color reversion) of soybean salad oil.
Almost all the precursors (such as tocored) were converted to color-reverted substances under catalysis after bleaching treatment,
and they were converted back to the precursors during deodorization. Those precursors might be gradually reconverted into
the color reverted substances during storage to cause the color reversion in soybean salad oil. 相似文献
905.
合成了一种疏水缔合水溶性聚丙烯酰胺共聚物,使用荧光光谱法并结合紫外及流变性实验,对制备的疏水缔合水溶性聚丙烯酰胺共聚物在水溶液中形成疏水微区、超分子聚集体及空间网络结构进行了研究,并用扫描电子显微镜证实了溶液中网络结构的存在. 相似文献
906.
907.
908.
C. H. Hsueh C. R. Luttrell S. Lee T. C. Wu H. Y. Lin 《Journal of the American Ceramic Society》2006,89(5):1632-1638
Stresses normal to interfaces, i.e., interfacial peeling stresses and interfacial shear stresses, exist locally at edges of multilayers because of both the thermal mismatch between layers and the free-edge effect. These peeling and shear stresses can result in modes I and II edge delamination, respectively. However, because of the complexity of the problem, exact closed-form solutions for these stresses are very difficult if not impossible to derive even for bilayered systems. Hence, instead of the detailed stress field at edges, both the interfacial peeling moment resulting from the localized peeling stresses and the interfacial shear force resulting from the localized shear stresses are analyzed here. Exact closed-form solutions for the peeling moment and the shear force at each interface in elastic multilayered systems are derived. To illustrate the application of present closed-form solutions, specific results are calculated for five-layered thermal barrier coating systems, and a finite-element analysis is also performed to confirm the analytical results. 相似文献
909.
We have examined the rheological properties of a Co-adsorbed γ-Fe2O3 magnetic suspension with polyurethane (PU) as a binder and methyl ethyl ketone (MEK) or cyclohexanone (CH) as a solvent. A Haake RV20 viscometer was used to measure the suspension viscosity and the vane method was adopted to determine the fluid yield stress. A rheological model which can be viewed as a combination of the Casson model and the Bingham model is proposed to describe the suspension viscosity. The effects of temperature, particle content, and binder concentration are included in the model. 相似文献
910.