Comprehensive Analysis of hsa-miR-654-5p’s Tumor-Suppressing Functions

The pivotal roles of miRNAs in carcinogenesis, metastasis, and prognosis have been demonstrated recently in various cancers. This study intended to investigate the specific roles of hsa-miR-654-5p in lung cancer, which is, in general, rarely discussed. A series of closed-loop bioinformatic functional analyses were integrated with in vitro experimental validation to explore the overall biological functions and pan-cancer regulation pattern of miR-654-5p. We found that miR-654-5p abundance was significantly elevated in LUAD tissues and correlated with patients’ survival. A total of 275 potential targets of miR-654-5p were then identified and the miR-654-5p-RNF8 regulation axis was validated in vitro as a proof of concept. Furthermore, we revealed the tumor-suppressing roles of miR-654-5p and demonstrated that miR-654-5p inhibited the lung cancer cell epithelial-mesenchymal transition (EMT) process, cell proliferation, and migration using target-based, abundance-based, and ssGSEA-based bioinformatic methods and in vitro validation. Following the construction of a protein–protein interaction network, 11 highly interconnected hub genes were identified and a five-genes risk scoring model was developed to assess their potential prognostic ability. Our study does not only provide a basic miRNA-mRNA-phenotypes reference map for understanding the function of miR-654-5p in different cancers but also reveals the tumor-suppressing roles and prognostic values of miR-654-5p.     

Nanocarrier-Loaded Imidaclothiz Promotes Plant Uptake and Decreases Pesticide Residue

There is a great demand for improving the effective utilization of pesticides and reducing their application for sustainable agriculture, and polymeric nanoparticles have provided strong technical support for the efficient delivery of pesticides. To this context, we tried to construct a relatively safe imidaclothiz nano-delivery system for enhanced plant uptake, reduced pesticide residue and improved bioactivity toward green peach aphids. The imidaclothiz could be assembled with the hydrophobic core of SPc through hydrophobic association, which led to the self-assembly of nanoscale imidaclothiz/SPc complex consisting of nearly spherical particles. The SPc decreased the contact angle of imidaclothiz drops and remarkably increased the plant uptake. Furthermore, the bioactivity and control efficacy of imidaclothiz were significantly improved with the help of SPc in both laboratory and field. Excitingly, the residue of imidaclothiz decreased with the help of SPc 7 d after the treatment due to the faster degradation of nanoscale imidaclothiz/SPc complex, which exhibited no negative effects on agronomic traits of tobacco plants. The current study successfully constructed a nano-delivery system for imidaclothiz, which can not only increase the effective utilization of pesticides, but also decrease the pesticide residue.     

低压配电系统无功集中补偿装置的应用

结合攀成钢炼铁厂3#高炉槽下低压配电室和循环水泵站低压供电系统对无功集中补偿装置的应用,阐述了无功补偿对输变电设备的作用,可提高运行技术指标,取得良好的经济效益。     

ANALYSIS OF SEEPAGE FLOW IN A CONFINED AQUIFER WITH A STANDING COLUMN WELL

The standing column well for ground source heat pump systems is a promising technology with high efficiency and environmental benefit, where groundwater is drawn from the bottom of a well and then re-injected to its top after transferring heat with heat pumps. Heat transfer analysis of great significance and aquifer involves complex problems. Determining the groundwater seepage flow is a precondition to solve the energy equation describing the heat transfer of the system. Only when piezometric head is obtained, the seepage velocity can be determined according to Darcy’s law. In this article the groundwater seepage flow in an axial symmetrical geometry was studied under the assumption that gross groundwater flow is neglected. An analytical solution of the groundwater seepage flow for a confined aquifer was acquired by using the integral transform method, which may provide a foundation for heat transfer analysis of the standing column well system.     

20%氟硅唑·咪鲜胺水乳剂防治黄瓜炭疽病

20%氟硅唑·咪鲜胺水乳剂防治黄瓜炭疽病的田间小区试验结果表明,在160～200ga.i./hm2剂量下,施药3～4次,间隔7～8d,可有效地控制黄瓜炭疽病的危害,并有提高产量和品质的作用。     

碳中和目标下电力需求预测体系构建及华东区域电力需求发展趋势研究

碳达峰、碳中和将加速全社会各行各业,尤其是能源系统的转型,而电力行业作为能源枢纽,能源领域在双碳转型过程中将对电力需求带来不可忽视的影响。该文立足于分析双碳目标对于能源电力的影响,挖掘双碳和电力需求的关联性,提出碳中和目标下分行业发展趋势分析思路,构建了一套适应于双碳目标下的电力电量预测方法体系;以华东区域作为典型案例,研究了华东区域碳达峰至碳中和过程中的电力需求发展趋势。研究结果表明,所构建的分析思路和方案体系科学可行,预测结论合理。     

基于WSR视角的PPP项目政府信用评价指标体系构建研究

政府和社会资本合作(PPP)模式在我国基础设施、公共事业等领域得到广泛应用.基于WSR系统方法论,从物理、事理、人理视角出发,利用层次分析法(AHP)和决策与实验室法(DEMATEL)设计、构建一套PPP项目政府信用评价指标体系.研究表明,PPP项目政府信用评价指标体系由物理、事理、人理三大部分组成,共计13个指标,其...     

人工智能在合成生物学的应用

生命系统极其复杂,难以精确描述和预测,这给高效设计合成生物系统提出了挑战,故在合成生物系统构建中往往须进行海量工程试错和优化.近年来,人工智能技术快速发展,其基于海量数据的持续学习能力和在未知空间的智能探索能力有效契合了当前合成生物学工程化试错平台的需求,在复杂生物特征的挖掘与生命系统的设计方面具备巨大潜力.该文回顾并...     

PCR在啤酒污染菌检测和鉴定中的应用

综述了PCR技术在啤酒污染菌鉴定中的应用,主要阐述了种或属的专一性PCR扩增和抗啤酒花质粒的跨种标记PCR扩增,三个抗啤酒花基因标记分别是horA、horB和horC,并介绍了论证抗啤酒花性的方法,它们分别是溴化乙锭和啤酒花的排溢试验,质粒重组试验和多个种中携带这三段基因的质粒比较试验等;还阐述了细菌抗啤酒花的主要机制,得出乳酸菌的抗啤酒花质粒可以通过基因的水平转移获得;最后通过比较两种PCR方法的优缺点,得出只有把两种方法结合起来使用才能在实际生产中产生加以应用.     

超高压处理对牡蛎中GII.4型诺如病毒的消减控制

诺如病毒（NoVs）是世界范围内引起非细菌性胃肠炎的主要病原体。为研究不同压力条件的超高压处理对牡蛎中NoVs消减控制的影响,将GⅡ.4型NoVs分别置于牡蛎消化腺匀浆和PBS缓冲液中,采用压力分别为200,300,400,500MPa,加压初始温度5℃,保压时间5 min进行处理,并以常压（0.1 MPa）处理为对照组。采用PMAxx-RT-qPCR与透射电子显微镜检测超高压处理后的NoVs,评价不同压力对牡蛎消化腺匀浆和PBS缓冲液中GⅡ.4型NoVs的消减效果;测定超高压处理后牡蛎丙二醛含量和蛋白质巯基含量,评价超高压处理对牡蛎中脂肪和蛋白质的影响。研究结果显示：PMAxx可有效区分感染性NoVs;qPCR检测发现经不同压力的超高压处理后,牡蛎消化腺匀浆和缓冲液中NoVs的RNA拷贝数均显著减少,当压力为500 MPa时,牡蛎和缓冲液中的RNA拷贝数减少量分别>3.49 lg(copies/μL)和>3.61 lg(copies/μL),均降至检测限以下;超高压处理对PBS缓冲液中NoVs的消减效果优于牡蛎消化腺匀浆,还可使牡蛎中脂肪氧化和蛋白质变性,然而,改变程度小...