Overexpression, purification, and characterization of SHPTP1, a Src homology 2-containing protein-tyrosine-phosphatase

A protein-tyrosine-phosphatase (PTPase; EC 3.1.3.48) containing two Src homology 2 (SH2) domains, SHPTP1, was previously identified in hematopoietic and epithelial cells. By placing the coding sequence of the PTPase behind a bacteriophage T7 promoter, we have overexpressed both the full-length enzyme and a truncated PTPase domain in Escherichia coli. In each case, the soluble enzyme was expressed at levels of 3-4% of total soluble E. coli protein. The recombinant proteins had molecular weights of 63,000 and 45,000 for the full-length protein and the truncated PTPase domain, respectively, as determined by SDS/PAGE. The recombinant enzymes dephosphorylated p-nitrophenyl phosphate, phosphotyrosine, and phosphotyrosyl peptides but not phosphoserine, phosphothreonine, or phosphoseryl peptides. The enzymes showed a strong dependence on pH and ionic strength for their activity, with pH optima of 5.5 and 6.3 for the full-length enzyme and the catalytic domain, respectively, and an optimal NaCl concentration of 250-300 mM. The recombinant PTPases had high Km values for p-nitrophenyl phosphate and exhibited non-Michaelis-Menten kinetics for phosphotyrosyl peptides.     

n-Al2O3/Ni复合镀层的组织与滑动磨损性能研究

用电刷镀技术制得了镍基n-Al2O3复合镀层，并对镀层的滑动磨损性能进行了试验研究。纳米复合镀层的表面形貌比较细腻，镀层中纳米粒子分布均匀，与基质金属结合紧密。镀层显微硬度达到HV700，比快速镍镀层提高约40％。滑动磨损试验结果表明，随着纳米粒子含量的增大，镀层的耐磨性提高，摩擦系数也呈增大趋势；但当镀层中n-Al2O3粒子的超过2.56%(质量分数）时，镀层的耐磨性显著下降。纳米复合镀层的磨损机制以疲劳磨损为主，而快速镍底层以粘着磨损为主。     

轴齿轮断齿失效分析

通过对轴齿轮断口形貌、硬度测试及金相组织等试验进行分析表明，齿根处没有进行硬化处理造成齿根处抗疲劳性能不足是导致轴齿轮疲劳断裂的主要原因，另外齿根过渡处倒角过小，且存在纵向刀痕引起了较大应力集中也是导致轴齿轮疲劳断裂的原因。     

On the coherent interference suppression using a spatiallysmoothing adaptive array

The behavior of a spatially smoothing adaptive array is examined. An expression for the weight vector is first derived. Using the array gain on the desired signal and the coherent interference is obtained. Then the expression for output signal-to-noise (SNR) is derived. It shows that the performance of the spatially smoothing array depends on the number of the subarrays, the angle separation, relative power and initial phase difference between the desired signal and the coherent interference. For good interference suppression it is found that the magnitude of the phase difference of the incident and interference signals must be greater than the beamwidths of both the subarray and the equivalent array. There is also a tradeoff between increasing the groups of subarrays and decreasing the number of elements in the subarrays. Computer simulation results are given that validate the analysis     

利用最小熵法预测高频拓宽频带

本根据最小熵观点给出了预测高频成分拓宽频带的方法，实际效果表明，该方法是一种拓宽频带的有效方法。     

Least mean p-power error criterion for adaptive FIR filter

An adaptive FIR filter based on the least mean p-power error (MPE) criterion is investigated. First, some useful properties of MPE function are studied. Three main results are as follows: 1) MPE function is a convex function of filter coefficients; so it has no local minima. 2) When input process and desired process are both Gaussian processes, then MPE function has the same optimum solution as the conventional Wiener solution for any p. 3) When input process and desired process are non-Gaussian processes, then MPE function may have better optimum solution than Wiener solution. Next, a least mean p-power (LMP) error adaptive algorithm is derived and some application examples are presented. Consequently, when the signal is corrupted by an impulsive noise, the adaptive algorithm with p=1 is preferred. Furthermore, when the signal is corrupted by noise or interference, the adaptive algorithm with proper choice of p may be preferred     

Generalized eigenvectors and fractionalization of offset DFTs and DCTs

The offset discrete Fourier transform (DFT) is a discrete transform with kernel exp[-j2/spl pi/(m-a)(n-b)/N]. It is more generalized and flexible than the original DFT and has very close relations with the discrete cosine transform (DCT) of type 4 (DCT-IV), DCT-VIII, discrete sine transform (DST)-IV, DST-VIII, and discrete Hartley transform (DHT)-IV. In this paper, we derive the eigenvectors/eigenvalues of the offset DFT, especially for the case where a+b is an integer. By convolution theorem, we can derive the close form eigenvector sets of the offset DFT when a+b is an integer. We also show the general form of the eigenvectors in this case. Then, we use the eigenvectors/eigenvalues of the offset DFT to derive the eigenvectors/eigenvalues of the DCT-IV, DCT-VIII, DST-IV, DST-VIII, and DHT-IV. After the eigenvectors/eigenvalues are derived, we can use the eigenvectors-decomposition method to derive the fractional operations of the offset DFT, DCT-IV, DCT-VIII, DST-IV, DST-VIII, and DHT-IV. These fractional operations are more flexible than the original ones and can be used for filter design, data compression, encryption, and watermarking, etc.     

职业道德建设的调查与思考

近年来各行各业加强职业道德建设,取得了一定成绩,涌现了一大批敬业爱岗、服务社会的岗位明星,但也存在着这样或那样的认识误区或现实局限,如形式主义、短期行为和领导观念异位等,这极大地阻碍了职业道德建设的深入推进。职业道德建设就其特征而言,它具有长期性、时代性和阶级性、相对稳定性以及创新性等属性,对此需要有一个理智而清晰的认识。应从营造良好的社会氛围、制定切实可行的道德准则、进行规范化的教育引导和发挥先进人物的示范作用等几方面入手,把职业道德建设推向深入。     

直齿圆柱变位齿轮的一种测绘方法

在工程实践中对损坏的无图纸齿轮进行重新制造时，测绘是必不可少的环节。在齿面已被严重磨损的情况下，如何准确地得到齿轮尤其是变位齿轮的各项参数是本文所着重讨论的问题。     

A new efficient numerical method and the dynamic analysis of composite laminates with piezoelectric layers

Firstly, a numerical method for the inversion of Laplace transform is developed and its accuracy is shown through examples. Then, a state-vector equation for the dynamic problems of piezoelectric plates is deduced directly from a modified mixed variational principle for piezoelectric bodies and its exact solution for the dynamic problems of simply supported rectangle piezoelectric plate is simply given. For multilayered hybrid plates, we derive the solution in terms of the propagator matrices. The techniques accounts for the compatibility of generalized displacements and generalized stresses on the interface both the elastic layers and piezoelectric layers, and the transverse shear deformation and the rotary inertia of laminate are also considered in the global algebraic equation of structure. Meanwhile, there is no restriction on the thickness and the number of layers. As an application of the numerical inversion of Laplace transform presented in this paper, typical numerical examples of the harmonic vibration and transient response are proposed and discussed. Since the highly accurate numerical results, they can serve as benchmarks to test various thick plate theories and various numerical methods, such as the finite and boundary element methods for transient response problems.