Delayed effects of chronic low-dose high linear energy transfer (LET) radiation on mice in vivo

In the present work, the delayed effects of chronic high linear energy transfer (LET) radiation in polychromatic erythrocytes (PCEs) of mice bone marrow were investigated in vivo. Irradiation of the two-month-old SHK white mongrel random-bred male mice was performed in the radiation field behind the concrete shield of the accelerator of 70 GeV protons to accumulate doses of 0.005-0.16 Gy. The dependence of the biological response on dose, adaptive response (AR) and genomic instability (GI) in F(1) and F(2) generations from males irradiated with doses of 0.005 and 0.16 Gy and from males exposed to combined action of immunomodulator-bendazol hydrochloride (BH) and of 0.16 Gy irradiation, were examined using the micronucleus formation test. The data demonstrated that irradiation of mice with these doses lead to an increase in the level of cytogenetic damage and induces no AR. With analysis of the bone marrow radiosensitivity to 1.5 Gy of X rays and the capacity to AR it was found that the chronic high-LET irradiation of parents induced the GI at least two generations. The combined exposure to BH and the dose of 0.16 Gy induces no AR in F(0) generation but induces AR in F(1) and F(2) offspring.     

Fluorescent ferritin nanoparticles and application to the aptamer sensor

We synthesized fluorescent ferritin nanoparticles (FFNPs) through bacterial expression of the hybrid gene consisting of human ferritin heavy chain (hFTN-H), spacer (glycine-rich peptide), and enhanced green (or red) fluorescent protein [eGFP (or DsRed)] genes. The self-assembly activity of hFTN-H that leads to the formation of nanoparticles (12 nm in diameter), the conformational flexibility of the C-terminus of hFTN-H, and the glycine-rich spacer enabled eGFPs (or DsReds) to be well displayed on the surface of each ferritin nanoparticle, resulting in the construction of green (or red) FFNPs [gFFNPs (or rFFNPs)]. As compared to eGFP (or DsRed) alone, it is notable that the developed FFNPs showed significantly amplified fluorescence intensity and also enhanced stability. DNA aptamers were chemically conjugated to gFFNP via each eGFP's cysteine residue that was newly introduced through site-directed mutagenesis (Ser175Cys). The DNA-aptamer-conjugated gFFNPs were used as a fluorescent reporter probe in the aptamer-based "sandwich" assay of a cancer marker [i.e., platelet-derived growth factor B-chain homodimer (PDGF-BB)] in phosphate-buffered saline buffer or diluted human serum. This is a simple two-step assay without any additional steps for signal amplification, showing that compared to the same aptamer-based assays using eGFP alone or Cy3, the detection signals, affinity of the reporter probe to the cancer marker, and assay sensitivity were significantly enhanced; i.e., the limit of detection was lowered to the 100 fM level. Although the PDGF-BB assay is reported here as a proof-of-concept, the developed FFNPs can be applied in general to any aptamer-based sandwich assays.     

智能煤矿矿用水泵状态检测的研究

结合智慧矿山的应用背景,针对MD580-70×9这一类型的矿用水泵,从故障判断、故障诊断以及信号预测三个方面出发,希望通过模拟水泵振动信号得到一种较为普适的应用模型,在时域利用模拟水泵进行故障的判断以及基于长短时记忆(LSTM)神经网络对时域信号进行预测,在判断出故障后通过FFT进行时频转换,进而判断出水泵故障类型,实现对矿用水泵进行检测。     

惰性气体超纯化过程中催化氧化痕量的一氧化碳和氢

在室温下工作的一台电子级气体纯化器最近由BOC集团技术中心研制成功。该纯化器可除掉几vppm的杂质,可使惰性气体中的CO、H_2、CO_2、H_2O和O_2等杂质含量降到<0.1vppm的水平。在研制过程中,为了选择最有效和最可靠的催化剂,考察了几种担载到载体上的金属催化剂在室温下氧化CO和H_2反应的催化行为。对于用担载Rh和Pt的催化剂,在低反应物浓度(<10vppm)和低温(20℃)下,其结果是过渡时间长,失活缓慢。如同在较高浓度和较高温度下氧化CO一样,观察到反应具有稳态的多重性,这被认为是反应机理的变化,此机理变化好象与失活过程本质有关。