HeLa cell transfection using a novel sonoporation system

Sonoporation has been shown to have an important role in biotechnology for gene therapy and drug delivery. This paper presents a novel microfluidic sonoporation system that achieves high rates of cell transfection and cell viability by operating the sonoporation chamber at resonance. The paper presents a theoretical analysis of the resonant sonoporation chamber design, which achieves sonoporation by forming an ultrasonic standing wave across the chamber. A piezoelectric transducer (PZT 26) is used to generate the ultrasound and the different material thicknesses have been identified to give a chamber resonance at 980 kHz. The efficiency of the sonoporation system was determined experimentally under a range of sonoporation conditions and different exposures time (5, 10, 15, and 20 s, respectively) using HeLa cells and plasmid (peGFP-N1). The experimental results achieve a cell transfection efficiency of 68.9% (analysis of variance, ANOVA, p < 0.05) at the resonant frequency of 980 kHz at 100 V(p-p) (19.5 MPa) with a cell viability of 77% after 10 s of insonication.     

气质联用法测定电视外壳中十种多溴二苯醚

建立了电视机外壳样品中10种多溴二苯醚的微波萃取-气质联用测定方法。10种多溴二苯醚的定量峰面积与其浓度在一定范围内成线性关系,检出限从0.08μg/m L到0.60μg/m L。采用索氏萃取法进行方法验证,测试结果的RSD≤2.83,两种方法经t检验无显著性差异。该方法具有简便、快速、试剂消耗量少的优点,可应用于电视机等国推污染控制认证产品塑料材料中多溴二苯醚测定。     

控制器局域网(CAN)的原理及其实施

CAN总线(控制器局域网)是适用于多数以CAN与ISO 11898作为物理层的理想的高级工业协议。其成本、性能及可升级性都为系统设计提供了极大的灵活性;LIN总线(局域互联网络)是一种低成本的新型串行通信系统,适用于汽车中的分布式电子控制系统,完善了现有的各种汽车多路网络。从本期开始,我们将精选部分有关CAN／LIN的技术文章,连续刊登在“CAN／LIN专栏”中,供广大读者参考。     

BSIM4和ULTRA-BULK模型对称性和连续性的检验

本文以对比的形式用GUMMEL对称测试电路的模拟结果检验了伯克利大学的BSIM4和北京大学的ULTRA-BULK两个CMOS器件模型的对称性和连续性特性。SPICE模拟结果表明: 工业标准模型BSIM4在电荷,电流高阶导数以及电容等的连续性和对称性上具有一系列的缺陷,而最新发展的基于表面势的MOSFET解析模型ULTRA-Bulk却表现出必需的连续性和对称性. 既然这些属性对于模拟电路和射频电路设计都是非常重要的, 那么新一代CMOS模型采用基于表面势的各种MOSFET解析模型将是必然的发展.     

用能量守恒定律消除高压变频室内负压

高压变频设备在我厂投运以来节能效果显著,但由于变频设备采用的室内外空气交换散热方式而产生的负压等一系列问题,不仅严重影响着生产的安全稳定运行,也危及着人身安全。我公司技术人员通过自主创新,采用能量守恒定律对变频设备的散热方式进行了改变,无需投资,见效快,投运一年来收到了良好的运行效果。     

在数字调制系统中进行精确的非线性测量

随着日益增加的智能手机和无线互联网3G覆盖范围以及4G系统即将引入带来的持续压力,数字通信射频组件的设计活动越来越丰富。设计活动的前沿在于功率放大器（PA）的开发。PA设计工程师面对的首要问题是功率所增加的效率（PAE）。高PAE意味着：     

Scaling and technological limitations of 1/f noise and oscillator phase noise in SiGe HBTs

This paper examines the impact of SiGe HBT scaling on 1/f noise and phase noise of oscillators and frequency synthesizers. The increase of transistor speed with scaling is shown to significantly increase the sensitivity of oscillation frequency to 1/f noise and, thus, degrade close-in phase noise, but decrease the sensitivity of oscillation frequency to base current shot noise and base resistance thermal noises. The results show that corner offset frequency defined by the intersect of the 1/f³ and 1/f² phase noise has little to do with the traditional 1/f corner frequency. The relative importance of individual noise sources in determining phase noise is examined as a function of technology scaling, device sizing, and oscillation frequency. The collector current shot noise and base resistance noise are shown to set the fundamental limits of phase noise reduction. A methodology to identify the maximum tolerable 1/f K factor is established and demonstrated for the HBTs used     

表面等离子激元脉冲整流产生太赫兹电动势

为了深入地研究表面等离子(Surface Plasmon,SP)在传播方向上的太赫兹(THz)电动势,采用了在纳米线结构上对表面等离子激元(Surface Plasmon Polariton,SPP)的脉冲中电子进行整流的方法,分析了SP感应引力的整流作用,讨论了SPP横向模式在长脉冲和短脉冲情况下的电动势模型。研究结果表明,脉冲整流会产生一个幅值较大的电动势,该电动势可达10 V。由于受纳米等离子的限制,在放大过程几乎是非共振的,考虑强纳米等离子限制和纵向SPP波局域条件下,整流作用在金属表面会产生更高的局域THz电场ER,其最大强度EmR=105~106V/cm。整流后SPP脉冲表现为快速,THz场频率,带宽5~20 THz的特性。SPP脉冲中THz电动势的分析对纳米级飞秒光学场的探测,半导体设备的纳米等离子激元耦合,以及非线性THz光谱研究都具有重要意义。     

Regulation and Reconstruction of Cell Phenotype Gradients Along the Tendon-Bone Interface

Tendon–bone interface is prevalent in the human body. It is divided into four zones: tendon (soft tissue), unmineralized fibrocartilage, mineralized fibrocartilage, and bone (hard tissue). Tendon–bone interface is characterized by a cell phenotype gradient that appears in the different zones. The cell phenotype gradients at the tendon–bone interface are orchestrated by specific intracellular molecular mechanisms, extracellular factors, immune signals, and neurovascular factors. These features have inspired scientists to design systems that mimic natural cell phenotype gradients. These biomimetic systems include the construction of cell sheets, regulation of cellular microenvironments, and the design of gradient functional scaffolds. Exploration of methods to mimic cell phenotype gradients is instructional for future clinical applications in reconstituting the tendon–bone interface. The present review elucidates the gradient composition of the tendon–bone interface. The associated regulatory mechanisms and applications are discussed, with the anticipation of creating a mise en scène for future research in interface tissue engineering.     

Cooperative and opportunistic transmission for wireless ad hoc networks

Moving toward 4G, wireless ad hoc networks receive growing interest due to users' provisioning of mobility, usability of services, and seamless communications. In ad hoc networks fading environments provide the opportunity to exploit variations in channel conditions, and transmit to the user with the currently "best" channel. In this article two types of opportunistic transmission, which leverage time diversity and multi-user diversity, respectively, are studied. Considering the co-channel interference and lack of a central controller in ad hoc networks, the "cooperative and opportunistic transmission" concept is promoted. For opportunistic transmission that exploits time diversity, it is observed that the inequality in channel contention due to the hidden terminal phenomenon tends to result in energy inefficiency. Under this design philosophy, we propose a distributed cooperative rate adaptation (CRA) scheme to reduce overall system power consumption. Taking advantage of the time-varying channel among different users/receivers and being aware of the potential contention among neighboring transmissions, we propose a QoS-aware cooperative and opportunistic scheduling (COS) scheme to improve system performance while satisfying QoS requirements of individual flows. Simulation results show that by leveraging node cooperation, our proposed schemes, CRA and COS, achieve higher network throughput and provide better QoS support than existing work