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Transglutaminase (TGase) was separated from the culture broth of an isolated strain of Streptoverticillium mobaraense. The crude enzyme was prepared by centrifugation, ultrafiltration, precipitation by alcohol, centrifugation and freeze‐drying. The yield after these processes was 65–70%. Then the enzyme was purified to homogeneity by chromatography on CM‐cellulose and Sephadex G‐75 on which the yields were about 70% and 80%, respectively; the purified folds reached 2.5–4.7 and 1.08–2.06, respectively. The molecular weight of this TGase was 39,500–40,100 Da by gel filtration chromatography. Optimum enzyme activity was observed in the pH range of 5.0–7.0, and it was maintained stable at 20–40C. The optimal temperature and pH was 52C and 6.0, respectively. At 1 mM and 5 mM metal ion or inhibitors concentration, TGase activity was strongly inhibited by Zn2+ and NEM, and not affected obviously by Ba2+, Ca2+, Co2+, Cu2+, Fe2+, Fe3+, Mg2+, Mn2+, Na+ as well as PMSF and EDTA. The effects of these additions on this TGase were compared with those of other microbial TGases. 相似文献
43.
Hydrate formation rate plays an important role in making hydrates for the storage and transport of natural gas. Micellar surfactant solutions were found to increase gas hydrate formation rate and storage capacity. With the presence of surfactant, hydrate could form quickly in a quiescent system and the energy costs of hydrate formation reduced. Surfactants (an anionic surfactant, a non‐ionic surfactant and their mixtures) and liquid hydrocarbons (cyclopentane and methylcyclohexane) were used to improve hydrate formation. The experiments of hydrate formation were carried out in the pressure range 3.69–6.82 MPa and the temperature range 274.05–277.55 K. The experimental pressures were kept constant during hydrate formation in each experimental run. The effect of anionic surfactant (sodium dodecyl sulphate (SDS)) on natural gas storage in hydrates is more pronounced compared to a non‐ionic surfactant (dodecyl polysaccharide glycoside (DPG)). The induction time of hydrate formation was reduced with the presence of cyclopentane (CP). Cyclopentane and methylcyclohexane (MCH) could increase hydrate formation rate, but reduced hydrate storage capacity The higher methylcyclohexane concentration, the lower the hydrate storage capacity. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
44.
本文主要是在线性解调系统中,利用信道中加性高斯白噪声正交分量(nc与Ns)两者正交性与其中之一(ns)经相移之后形成线性相关性使噪声本身消除噪声,以达到提高信噪比的目的,从而提高抗噪声性能. 相似文献
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已往库存论中的决策模型,它是把现实过程用数学的方法进行分析,得到决策值,但不能看到决策的过程,不能反映出现实生活中的不确定性、动态性。运用Delphi 7.0和SQL server 2000对销售过程进行了动态仿真,利用Newsboy模型的推广(S,s)订购策略来监控库存并做出决策,这样就体现出此过程的不确定性、动态性;且举出了一个仿真商品销售的实例,对其中的一些关键技术,如何仿真、算法的实现等进行了阐述。 相似文献
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Sun Qing-Ping Guo Tian-Fu Li Xue-Jun Zhang Xing 《International Journal of Fracture》1996,78(3-4):315-330
Numerical study by the finite element method (FEM) is performed to investigate the effect of dual-scale microstructure on the toughness of laminar zirconia composite. The computation is based on the micromechanics constitutive model of polycrystal transformation plasticity developed by Sun et al. [10] where both transformation induced shear and softening effects during autocatalytic transformation are taken into account. The numerical simulation presented in this paper successfully reproduced the experimentally observed two effects of the dual-scale microstructure on the toughness of laminar zirconia composite, i.e., the truncation of the elongated transformation frontal zone that forms in single phase Ce-ZrO2 and the propagation of the transformation zone along the layers normal to the crack plane. Quantitative analysis on the role of microstructure in transformation toughening of laminar zirconia composite is first carried out in the present work which will provide a starting point for the microstructural design of this novel advanced composite in the future.Presented at the Far East Fracture Group (FEFG) International Symposium on Fracture and Strength of Solids. 4–7 July 1994 in Xi'an, China. 相似文献