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31.
Fitriyanto NA Fushimi M Matsunaga M Pertiwiningrum A Iwama T Kawai K 《Journal of Bioscience and Bioengineering》2011,111(6):613-617
The molecular structure and nucleotide sequence of Ce(3+)-induced methanol dehydrogenase (MDH) of Bradyrhizobium sp. MAFF211645 were investigated. The addition of 30 μM Ce(3+) to 1/10 nutrient broth containing 0.5% methanol remarkably increased MDH activity. Furthermore, La(3+) increased MDH activity, but other heavier rare earth and metal elements did not have the same effect. MDH increased by Ce(3+) was purified by sequential column chromatography, and the purified MDH migrated as a single band with an apparent molecular weight of 68 kDa on SDS-PAGE. The apparent molecular weight of native MDH was estimated to be 108,000 by gel chromatography. The MDH was comprised of two identical subunits. N-terminal 23-amino acid sequence, 1-NDELHKMAQNPKDWVMPAGDYAN-23, of the purified MDH exhibited 91.3% identity to that of the MDH large subunit-like protein encoded by mxaF' of Bradyrhizobium japonicum USDA110. Nucleotide sequencing of the MDH gene of strain MAFF211645 yielded a deduced amino acid sequence comprising 601 amino acid residues, an N-terminal signal peptide, and a mature MDH comprising 578 amino acid residues with a predicted molecular mass of 62,918 Da. Further analysis of the deduced amino acid sequence of mature MDH revealed that the functional amino acids in its active site, such as two adjacent Cys residues, and bacterial quinoprotein signatures 1 and 2 were conserved. These results indicate that Ce(3+)-induced MDH encoded by mxaF' may be involved in methanol metabolism in Bradyrhizobium sp. MAFF211645. 相似文献
32.
Agus EL Young DT Lingard JJ Smalley RJ Tate JE Goodman PS Tomlin AS 《The Science of the total environment》2007,386(1-3):65-82
Measurements of urban particle number concentrations and size distributions in the range 5-1000 nm were taken at elevated (roof-level) and roadside sampling sites on Narborough Road in Leicester, UK, along with simultaneous measurements of traffic, NO(x), CO and 1,3-butadiene concentrations and meteorological parameters. A fitting program was used to determine the characteristics of up to five modal groups present in the particle size distributions. All particle modal concentrations peaked during the morning and evening rush hours. Additional events associated with the smallest mode, that were not observed to be connected to primary emissions, were also present suggesting that this mode consisted of newly formed secondary particles. These events included peaks in concentration which coincided with peaks in solar radiation, and lower concentrations of the larger modes. Investigation into the relationships between traffic flow and occupancy indicated three flow regimes; free-flow, unstable and congested. During free-flow conditions, positive linear relationships existed between traffic flow and particle modal number concentrations. However, during unstable and congested periods, this relationship was shown to break-down. Similar trends were observed for concentrations of the gas phase pollutants NO(x), CO and 1,3-butadiene. Strong linear relationships existed between NO(x), CO, 1,3-butadiene concentrations, nucleation and Aitken mode concentrations at both sampling locations, indicating a local traffic related emission source. At the roadside, both nucleation and Aitken mode are best represented by a decreasing exponential function with wind speed, whereas at the roof-level this relationship only occurred for Aitken mode particles. The differing relationships at the two sampling locations are most likely due to a combination of meteorological factors and distance from the local emission source. 相似文献
33.
Gu Qian Jiang PingPing Shen Yirui Zhang Kai Wai Phyu Thin Haryono Agus 《Journal of Porous Materials》2021,28(3):779-789
Journal of Porous Materials - Highly dispersed molybdenum trioxide were loaded in three-dimensional dendritic mesoporous silica nanospheres (MoO3@3D-DMSNs) by one-pot method. The mesoporous silica... 相似文献
34.
Agus Saptoro Morgan Ting Hock Herng Emily Liew Wan Teng 《Korean Journal of Chemical Engineering》2014,31(4):650-658
Experimental and mass transfer studies of oxygen transfer to cassava starch solution in an aerated, well-mixed bioreactor of 2 L have been carried out. The volumetric mass transfer coefficient was estimated and parametric studies were performed to study the effect of process variables of stirring rate, aeration rate, concentration of starch and temperature on the volumetric mass transfer coefficient. From the experimental results, it is evident that the first two and the last variables are directly proportional to the volumetric mass transfer coefficient. However, the volumetric mass transfer coefficient is inversely proportional to the concentration of the starch. Also, the saturation dissolved oxygen concentration is greatly affected by temperature and starch solution concentration. Whereas, stirring and aeration rates have neutral impacts on saturation dissolved oxygen concentration. Simulated data generated from obtained volumetric mass transfer coefficient agrees well with the experimental data, which indicates the accuracy of the coefficient. 相似文献
35.
Agus R. Poerwoprajitno Lucy Gloag Tania M. Benedetti Soshan Cheong John Watt Dale L. Huber J. Justin Gooding Richard D. Tilley 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(17)
Branched nanoparticles are one of the most promising nanoparticle catalysts as their branch sizes and surfaces can be tuned to enable both high activity and stability. Understanding how the crystallinity and surface facets of branched nanoparticles affect their catalytic performance is vital for further catalyst development. In this work, a synthesis is developed to form highly branched ruthenium (Ru) nanoparticles with control of crystallinity. It is shown that faceted Ru branched nanoparticles have improved stability and activity in the oxygen evolution reaction (OER) compared with polycrystalline Ru nanoparticles. This work achieves a low 180 mV overpotential at 10 mA cm?2 for hours, demonstrating that record‐high stability for Ru nanocrystals can be achieved while retaining high activity for OER. The superior electrocatalytic performance of faceted Ru branched nanoparticles is ascribed to the lower Ru dissolution rate under OER conditions due to low‐index facets on the branch surfaces. 相似文献
36.
Marco Agus Fabio Bettio Andrea Giachetti Enrico Gobbetti José Antonio Iglesias Guitián Fabio Marton Jonas Nilsson Giovanni Pintore 《The Visual computer》2009,25(9):883-893
We present a prototype medical data visualization system exploiting a light field display and custom direct volume rendering
techniques to enhance understanding of massive volumetric data, such as CT, MRI, and PET scans. The system can be integrated
with standard medical image archives and extends the capabilities of current radiology workstations by supporting real-time
rendering of volumes of potentially unlimited size on light field displays generating dynamic observer-independent light fields.
The system allows multiple untracked naked-eye users in a sufficiently large interaction area to coherently perceive rendered
volumes as real objects, with stereo and motion parallax cues. In this way, an effective collaborative analysis of volumetric
data can be achieved. Evaluation tests demonstrate the usefulness of the generated depth cues and the improved performance
in understanding complex spatial structures with respect to standard techniques.
相似文献
Enrico GobbettiEmail: URL: http://www.crs4.it/vic/ |
37.
Guy Malmary Joël Albet Agus Putranto Hafida Hanine Jacques Molinier 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2000,75(12):1169-1173
The recovery of aconitic and lactic acids from dilute aqueous effluents of the sugar‐cane industry by a solvent extraction technique has been investigated in order to reduce environmental pollution and in view of the possible uses of pure solutes in the field of foods and pharmaceuticals. Reactive extraction of carboxylic acids with tributylphosphate dissolved in dodecane was examined. Experiments were performed at 25 °C for three extractant concentrations of 50%, 60% and 70% (mass %) in dodecane. The decrease of specific gravity and viscosity of solvent resulting from the presence of an inert diluent was favourable to the phase settling and the mass transfer. The percentage of acids recovered in the organic phase for a volumetric solvent ratio varying from 0.5 to 5.0 was determined. Taking into account the difference between the partition coefficients for the carboxylic acids under consideration, the selectivity of solvent with regard to aconitic acid also was quantified. Moreover, owing to its water‐insolubility, its availability and its low cost, tributylphosphate can be considered as a promising extractant for large‐scale applications. © 2000 Society of Chemical Industry 相似文献
38.
The operation of proton exchange membrane fuel cell (PEMFC) stacks requires careful thermal and water management for optimal performance. Appropriate placement of cooling plates and appropriate cooling conditions are therefore essential. To study the impact of these design parameters, a two-phase model accounting for the conservation of mass, momentum, species, energy, and charge, a phenomenological model for the membrane, and an agglomerate model for the catalyst layer, is developed and solved. The model is validated for a single cell, in terms of both the local and the global current density, and good agreement is found. Four repetitive computational units are then identified for the number of single cells placed between the coolant plates: (i) one cell; (ii) two cells; (iii) three cells; and (iv) four cells. The flow fields in the single cells and the cooling plates are of a net type. The results show that there is a strong correlation between stack performance and the operating conditions/placement of the coolant plates. For the limiting case of one coolant plate between each unit cell, similar operating conditions can be achieved in every individual cell throughout the stack. As more cells are placed in between coolant plates, the stack performance drops due to an increase in temperature and decrease in water content in the membranes, unless the cooling temperature is lowered. The coolant temperature and inlet velocity need to be monitored carefully and adjusted to the operating conditions of the stack. This model can be employed for design and optimization of liquid water cooling of a PEMFC stack. 相似文献
39.
Muhammad Ghozali Samsul Bakhri Evi Triwulandari Witta Kartika Restu Yenny Meliana Agus Haryono Melati Septiyanti Nissa Nurfajrin Solihat Seng Hua Lee Widya Fatriasari Petar Antov 《应用聚合物科学杂志》2024,141(3):e54803
Conventional polyurethane (PU) is usually synthesized by a reaction between isocyanate and polyol. The use of isocyanate compounds is associated with significant health and environmental problems. Therefore, it is necessary to develop an environmentally friendly alternative method for manufacturing PUs without isocyanate routes. The aim of this research work was to synthesize green urethane from oleic acid, which included the following three stages: the synthesis of epoxidized oleic acid (EOA), the synthesis of carbonated oleic acid (COA), and the synthesis of green urethane from oleic acid (UOA). The resulting product was characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Nuclear Magnetic Resonance (NMR) analyses, and by determining the iodine number, oxirane number, and hydroxyl value. The results of FTIR and NMR showed that EOA was successfully synthesized. The optimum COA synthesis process was obtained on TBAB catalyst usage of 1% (wt/wt) at 140°C for 48 h with a 500-rpm stirring rate and CO2 gas flow rate of 0.2 mL/min with the resulting COA oxirane value of 0.00. The optimum condition of UOA synthesis through the aminolysis process resulted in the use of LiCl of 19.8% (wt/wt) at 70°C for 3 h with a stirring speed of 1200 rpm with a UOA hydroxyl number generated of 237.93 mg/mL. 相似文献
40.