This paper presents a vision-based method for automatic tracking of biological cells in time-lapse microscopy by combining the motion features with the topological features of the cells. The automation of tracking frequently faces problems of segmentation error and of finding correct cell correspondence in consecutive frames, since the cells are of varying size and shape, and may have uneven movement; these problems become more acute when the cell population is very high. To reduce the segmentation error, we introduce a cell-detection method based on h-maxima transformation, followed by the fitting of an ellipse for the nucleus shape. To find the correct correspondence between the detected cells, the topological features, namely, color compatibility, area overlap and deformation are combined with the motion features of skewness and displacement. This reduces the ambiguity of matching and constructs accurately the trajectories of the cell proliferation. Finally, a template-matching-based backward tracking procedure is employed to recover any break in a cell trajectory that may occur due to the segmentation errors or the presence of a mitosis. The tracking procedure is tested using a number of different cell sequences with nonuniform illumination, or uneven cell motion, and is shown to provide high accuracy both in the detection and the tracking of the cells. 相似文献
In this paper, we introduce a new hybrid ARQ technique for broadcasting or multicasting in erasure channel. The system is tested according to the objective criteria—quantity of information sent by the source, loses, and number of negative acknowledgments (NACKs) sent by the receiver nodes (end nodes). We compare our proposed method with automatic repeat request (ARQ), hybrid ARQ II (HARQ II), and also with a forward error correction (FEC) transmission technique based on Reed Solomon code (RS). The main focus of the presented HARQ is to reduce the quantity of redundant information sent by the source as well as the number of NACKs sent by the receivers, maintaining the condition that all the information is being recovered successfully by the receivers. 相似文献
One-dimensional manganese (IV) oxide (MnO2) (~20 nm in average diameter) were synthesized by cathodic electrodeposition and heat treatment. The mechanism of electrodeposition and nanowire formation were discussed. The product was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared (FT-IR). Nanowires with varying lengths and diameters were found in TEM and SEM images of the sample. The results of N2 adsorption–desorption analysis indicated that the BET surface area of the MnO2 nanowires was 157 m2 g?1 and the pore size distributions were 2.5 and 4.5 nm. The electrochemical performances of the prepared MnO2 as an electrode material for supercapacitors were evaluated by cyclic voltammetry and galvanostatic charge/discharge measurements in a solution of 0.5 M Na2SO4. The higher specific capacitance of 318 F g?1 and good capacity retention of 86% were achieved after 1000 charge–discharge cycles had been observed for the MnO2 nanowires electrode. 相似文献
The H.264/AVC video coding standard uses multiple reference frames and variable-size macroblock partitions in inter-prediction. This flexibility allows the standard to achieve excellent rate-distortion performance at the cost of high encoding complexity. We present an algorithm for fast joint selection of the reference frames and macroblock partitions in rate-distortion-based coding. Experimental results for eight standard test video sequences show that, compared with exhaustive search, our algorithm can save up to 74.9 % of the encoding time with negligible loss in rate-distortion performance. The presented algorithm is also evaluated using a subjective assessment metric; quality evaluation measures based on user satisfaction. Diverse types of video sequences were used with different frame rates, quantization parameters, and resolutions. The effects of changing bit rate and resolution on compression efficiency and viewers’ satisfaction are also presented. Results show that our algorithm provides high scores of perceptual satisfaction that are significantly affected by the compression technique. As a result, we claim that our algorithm presents original and significant enhancement compared with exhaustive search. In addition, overall test results showed that our technique outperformed three of the best previously proposed methods and gave higher viewer satisfaction. 相似文献
Due to the significant advancement of Smartphone technology, the applications targeted for these devices are getting more and more complex and demanding of high power and resources. Mobile cloud computing (MCC) allows the Smart phones to perform these highly demanding tasks with the help of powerful cloud servers. However, to decide whether a given part of an application is cost-effective to execute in local mobile device or in the cloud server is a difficult problem in MCC. It is due to the trade-off between saving energy consumption while maintaining the strict latency requirements of applications. Currently, 5th generation mobile network (5G) is getting much attention, which can support increased network capacity, high data rate and low latency and can pave the way for solving the computation offloading problem in MCC. In this paper, we design an intelligent computation offloading system that takes tradeoff decisions for code offloading from a mobile device to cloud server over the 5G network. We develop a metric for tradeoff decision making that can maximize energy saving while maintain strict latency requirements of user applications in the 5G system. We evaluate the performances of the proposed system in a test-bed implementation, and the results show that it outperforms the state-of-the-art methods in terms of accuracy, computation and energy saving. 相似文献
In radio frequency identification (RFID) systems, search protocols are used to find a specific item in a large number of tagged products. These protocols should be secure against RFID attacks such as traceability, impersonation, DoS and eavesdropping. Sundaresan et al. (IEEE Trans Dependable Secure Comput, 2015) presented a server-less search protocol based on 128-bits PRNG function and claimed that their method can address all vulnerabilities of previous protocols. In this paper, we prove that Sundaresan et al.’s protocol is vulnerable to traceability attack with the high probability. In addition, we present an improved protocol to solve the proposed problem and analyze its security level informally and formally based on AVISPA tool and BAN logic. 相似文献
Dedicated short-range communications (DSRC) is an important wireless technology for current and future automotive safety and mitigation of traffic jams. In this work, we have designed a Coplanar waveguide microstrip patch antenna with linear, upper and bottom and side slots for application in DSRC. The patch antenna was designed using glass epoxy substrate (FR4). Various parametric analyses such as the current distribution, reflection coefficient, radiation pattern on E- and H-plane as well as the realized gain (dB) were performed. The results were obtained by simulation using high-frequency structure simulator tool. The proposed antenna covers a frequency band of 5.8–5.9 GHz which is highly dedicated to the DSRC wireless communication technology for enhancement of safety of the automotive transport system. The designed antenna shows a good return loss of ??19 dB at 5.9 GHz.The designed antenna shows a promising gain, return loss and radiation pattern for use in DSRC for automotive transport systems.
Wireless Personal Communications - Wireless Underground Networks comprise the ability to constantly monitor several physical parameters such as ground temperature, water level and soil condition,... 相似文献