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41.
A simple and rapid methodology for the preparation of single-cell suspension from animal tissues is described. It uses a device called a "tissue press" to mince the tissue into small pieces of desired sizes which are then dissociated into single cells using a pipette. The device is inexpensive and the method economizes time and labor. An example of its use in DNA double-strand breaks study, using microgel electrophoresis, is described.  相似文献   
42.
This paper proposes the Mobility-Aware Resource Reservation Protocol (MARSVP) in which mobility and QoS signaling are performed as a single functional block. The key concept of MARSVP is to convey mobility-specific information (binding updates and their associated acknowledgments) by using newly defined RSVP objects embedded in existing RSVP messages. An appealing feature of MARSVP is that it adheres to the current RSVP standard (RFC 2205) and thus requires minimal changes to end nodes without affecting any of the conventional RSVP routers in between. The proposed mechanism is evaluated using a simulation model for application-level performance and an analytical model for network-level signaling cost. Simulation results indicate a 27.9% improvement in QoS interruption when using Mobile IPv6 (MIPv6), 12.5% when using Hierarchical Mobile IPv6 (HMIPv6), and no improvement when using Fast Handovers for MIPv6 (FMIPv6). On the network-level, signaling cost savings of 9.4% and 11.9% are achieved for MIPv6 and HMIPv6, respectively, while FMIPv6 achieves savings of 17.9% when using Voice-over-IP traffic and 26.7% for Video-over-IP traffic. The results of the conducted studies indicate MARSVP’s superiority to conventional RSVP when deployed over wireless networks.  相似文献   
43.
In this paper, we have made medical application of a new artificial immune system named the information gain based artificial immune recognition system (IG-AIRS) which minimizes the negative effects of taking into account all attributes in calculating Euclidean distance in shape–space representation which is used in many artificial immune systems. For medical data, thyroid disease data set was applied in the performance analysis of our proposed system. Our proposed system reached 95.90% classification accuracy with 10-fold CV method. This result ensured that IG-AIRS would be helpful in diagnosing thyroid function based on laboratory tests, and would open the way to various ill diagnoses support by using the recent clinical examination data, and we are actually in progress.  相似文献   
44.
Mode choice modeling is probably the most important element of transportation planning. It affects the general efficiency of travel and the allocation of resources. The development of mode choice models has recently witnessed significant advances in many fields, such as passenger and freight transport. A large number of mathematical models have been used to model the traveler’s choice of mode and destination and the shipper’s choice of mode, shipment size and supply market, among others. Such models are not only becoming almost intractable but also data intensive, difficult to calibrate and update, and intransferable. These models cover a wide range of mathematical complexity and accuracy. This paper describes a new approach to mode choice of intercity freight transport modeling using artificial neural networks (ANNs) and adaptive neuro-fuzzy inference system (ANFIS) models. The new approach combines the learning ability of artificial neural networks and the transparent nature of fuzzy logic. The approach is found to be highly adaptive and efficient in investigating non-linear relationships among different variables. The adaptive neuro-fuzzy inference system model is tested on the freight transport market in Turkey, Germany, France and Austria by using information on the freight flows and their attributes. The ANNs and ANFIS models are more successful in the representation of the non-linear behavior of mode choice of intercity freight transport compared to the classical models.  相似文献   
45.
In wind energy conversion systems, one of the operational problems is the changeability and discontinuity of wind. In most cases, wind speed can fluctuate rapidly. Hence, quality of produced energy becomes an important problem in wind energy conversion plants. Several control techniques have been applied to improve the quality of power generated from wind turbines. Pitch control is the most efficient and popular power control method, especially for variable-speed wind turbines. It is a useful method for power regulation above the rated wind speed. This paper proposes an artificial neural network-based pitch angle controller for wind turbines. In the simulations, a variable-speed wind turbine is modeled, and its operation is observed by using two types of artificial neural network controllers. These are multi-layer perceptrons with back propagation learning algorithm and radial basis function network. It is shown that the power output was successfully regulated during high wind speed, and as a result overloading or outage of the wind turbine was prevented.  相似文献   
46.
Biodiesel is receiving increasing attention each passing day because of its fuel properties and compatibility with the petroleum-based diesel fuel (PBDF). Therefore, in this study, the prediction of the engine performance and exhaust emissions is carried out for five different neural networks to define how the inputs affect the outputs using the biodiesel blends produced from waste frying palm oil. PBDF, B100, and biodiesel blends with PBDF, which are 50% (B50), 20% (B20) and 5% (B5), were used to measure the engine performance and exhaust emissions for different engine speeds at full load conditions. Using the artificial neural network (ANN) model, the performance and exhaust emissions of a diesel engine have been predicted for biodiesel blends. According to the results, the fifth network is sufficient for all the outputs. In the fifth network, fuel properties, engine speed, and environmental conditions are taken as the input parameters, while the values of flow rates, maximum injection pressure, emissions, engine load, maximum cylinder gas pressure, and thermal efficiency are used as the output parameters. For all the networks, the learning algorithm called back-propagation was applied for a single hidden layer. Scaled conjugate gradient (SCG) and Levenberg–Marquardt (LM) have been used for the variants of the algorithm, and the formulations for outputs obtained from the weights are given in this study. The fifth network has produced R2 values of 0.99, and the mean % errors are smaller than five except for some emissions. Higher mean errors are obtained for the emissions such as CO, NOx and UHC. The complexity of the burning process and the measurement errors in the experimental study can cause higher mean errors.  相似文献   
47.
Reconstructing high dynamic range (HDR) images of a complex scene involving moving objects and dynamic backgrounds is prone to artifacts. A large number of methods have been proposed that attempt to alleviate these artifacts, known as HDR deghosting algorithms. Currently, the quality of these algorithms are judged by subjective evaluations, which are tedious to conduct and get quickly outdated as new algorithms are proposed on a rapid basis. In this paper, we propose an objective metric which aims to simplify this process. Our metric takes a stack of input exposures and the deghosting result and produces a set of artifact maps for different types of artifacts. These artifact maps can be combined to yield a single quality score. We performed a subjective experiment involving 52 subjects and 16 different scenes to validate the agreement of our quality scores with subjective judgements and observed a concordance of almost 80%. Our metric also enables a novel application that we call as hybrid deghosting, in which the output of different deghosting algorithms are combined to obtain a superior deghosting result.  相似文献   
48.
Every quadratic programming problem with a mix of continuous and binary variables can be equivalently reformulated as a completely positive optimization problem, that is, a linear optimization problem over the convex but computationally intractable cone of completely positive matrices. In this paper, we focus on general inner approximations of the cone of completely positive matrices on instances of completely positive optimization problems that arise from the reformulation of mixed binary quadratic programming problems. We provide a characterization of the feasibility of such an inner approximation as well as the optimal value of a feasible inner approximation. In particular, our results imply that polyhedral inner approximations are equivalent to a finite discretization of the feasible region of the original completely positive optimization problem. Our characterization yields, as a byproduct, an upper bound on the gap between the optimal value of an inner approximation and that of the original instance. We discuss the implications of this error bound for standard and box-constrained quadratic programs as well as general mixed binary quadratic programs with a bounded feasible region.  相似文献   
49.
Scalability in simulation tools is one of the most important traits to measure performance of software. The reason is that today’s Internet is the main instance of a large-scale and highly complex system. Simulation of Internet-scale network systems has to be supported by any simulation tool. Despite this fact, many network simulators lacks support for building large models. In this work, in order to propose a new approach for scalability issue in network simulation tools, a network simulator is developed based on behavior of honeybees and high performance DEVS, modular and hierarchical system theoretic approach. A biologically-inspired discrete event modeling approach is described for studying networks’ scalability and performance traits. Since natural systems can offer important concepts for modeling network systems, key adaptive and emergent attributes of honeybees and their societal properties are incorporated into a set of simulation models that are developed using the discrete event system specification approach. Large-scale network models are simulated and evaluated to show the benefits of nature-inspired network models.  相似文献   
50.
Multiple access interference (MAI) is the main factor affecting the performance of channel estimation techniques for code division multiple access (CDMA) systems. Although, several multi-user channel estimation algorithms have been proposed to mitigate MAI, these algorithms require high computational complexities. In this paper, we address the problem of iterative least squares (LS) mobile channel estimation at high channel efficiency that requires a short training sequence along with the spreading sequences. We employ an efficient iterative method based on conjugate gradient (CG) algorithm to reduce the computational complexity of the estimation method. Computer simulations illustrate that the proposed method performs almost identical to the exact LS estimate for reasonable training lengths.  相似文献   
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