cDNA cloning and functional characterization of the mouse Ca2+-gated K+ channel, mIK1. Roles in regulatory volume decrease and erythroid differentiation

We have cloned from murine erythroleukemia (MEL) cells, thymus, and stomach the cDNA encoding the Ca2+-gated K+ (KCa) channel, mIK1, the mouse homolog of hIK1 (Ishii, T. M., Silvia, C., Hirschberg, B., Bond, C. T., Adelman, J. P., and Maylie, J. (1997) Proc. Natl. Acad. Sci.(U. S. A. 94, 11651-11656). mIK1 mRNA was detected at varied levels in many tissue types. mIK1 KCa channel activity expressed in Xenopus oocytes closely resembled the Kca of red cells (Gardos channel) and MEL cells in its single channel conductance, lack of voltage-sensitivity of activation, inward rectification, and Ca2+ concentration dependence. mIK1 also resembled the erythroid channel in its pharmacological properties, mediating whole cell and unitary currents sensitive to low nM concentrations of both clotrimazole (CLT) and its des-imidazolyl metabolite, 2-chlorophenyl-bisphenyl-methanol, and to low nM concentrations of iodocharybdotoxin. Whereas control oocytes subjected to hypotonic swelling remained swollen, mIK1 expression conferred on oocytes a novel, Ca2+-dependent, CLT-sensitive regulatory volume decrease response. Hypotonic swelling of voltage-clamped mIK1-expressing oocytes increased outward currents that were Ca2+-dependent, CLT-sensitive, and reversed near the K+ equilibrium potential. mIK1 mRNA levels in ES cells increased steadily during erythroid differentiation in culture, in contrast to other KCa mRNAs examined. Low nanomolar concentrations of CLT inhibited proliferation and erythroid differentiation of peripheral blood stem cells in liquid culture.     

Quantitative electroencephalographic characteristics of crack cocaine dependence

This study replicates preliminary findings reporting a quantitative electroencephalographic (QEEG) profile of crack cocaine dependence in abstinence. All subjects (n = 52) met criteria for DMS-III-R cocaine dependence (in the form of crack), and were residing in a drug-free therapeutic community. Baseline QEEG evaluations were conducted at intake (5-10 days after last use of crack, and at follow-up (1 month after last reported use). Previous findings of significant excess of relative alpha power and deficit of absolute and relative delta and theta power were replicated in this expanded group. Abnormalities were greater in anterior than posterior regions, and disturbances in interhemispheric relationships were also observed. Further, QEEG showed little change in the interval between the first and second evaluations. This QEEG profile may reflect persistent alterations in neurotransmission as a possible consequence of chronic cocaine exposure.     

Elliptic cylinder geometry for distinguishability analysis in impedance tomography

Electrical impedance tomography (EIT) is a technique that computes the cross-sectional impedance distribution within the body by using current and voltage measurements made on the body surface. It has been reported that the image reconstruction is distorted considerably when the boundary shape is considered to be more elliptical than circular as a more realistic shape for the measurement boundary. This paper describes an alternative framework for determining the distinguishability region with a finite measurement precision for different conductivity distributions in a body modeled by elliptic cylinder geometry. The distinguishable regions are compared in terms of modeling error for predefined inhomogeneities with elliptical and circular approaches for a noncircular measurement boundary at the body surface. Since most objects investigated by EIT are noncircular in shape, the analytical solution for the forward problem for the elliptical cross section approach is shown to be useful in order to reach a better assessment of the distinguishability region defined in a noncircular boundary. This paper is concentrated on centered elliptic inhomogeneity in the elliptical boundary and an analytic solution for this type of forward problem. The distinguishability performance of elliptical cross section with cosine injected current patterns is examined for different parameters of elliptical geometry.     

Effect of copper on boride layer of boronized ductile cast irons

GGG-50, GGG-60 and GGG-80 ductile cast irons containing 0.01, 0.3 and 0.98 wt% copper, respectively, were boronized in a salt bath and then analyzed using optical microscopy, scanning electron microscopy and X-ray diffraction analysis. Increasing copper concentration in ductile cast irons resulted in formation of mono-phase boride layer (Fe₂B), decreased Si-ferrite zone and hindered the growth discontinuous graphite between boride layer and matrix.     

Effect of heat treatment in air on the thermal properties of SiC fibre-reinforced composite. Part 1: a barium osumilite (BMAS) matrix glass ceramic composite

The thermal properties have been studied on a glass ceramic composite comprised of a barium osumilite (BMAS) matrix reinforced with SiC (Tyranno) fibres which has been subjected to a heat treatment in air in the range of 700–1,200 °C. Microstructural studies were carried out especially on of the interface between fibre and matrix. The presence of a carbon thin layer in the interface is a typical observation in SiC fibre-reinforced glass ceramic matrix composite systems. The microstructural evaluation and thermal properties showed a degradation of interfacial layer occurred at low heat treatment temperatures, (700–800 °C) this was attributed to the fact that, at those heat treatment temperatures the carbon rich layer formed during processing was oxidised away leaving voids between fibre and matrix, which were linked by isolated silicon-rich bridges. After heat treatment at higher temperatures of 1,000–1,200 °C, the thermal properties were retained or even enhanced by leaving a thick interfacial layer.     

Investigation of thermoluminescence properties of metal oxide doped lithium triborate

In this study, lithium triborate (LiB₃O₅) doped with different metal oxides were investigated to explore its thermoluminescence properties. Solid-state reaction method was employed for the synthesis of the desired materials. The formation of the produced phases was confirmed by Powder X-Ray Diffraction (XRD), Infrared (IR), Differential Thermal Analysis (DTA) and Scanning Electron Microscopy (SEM) examinations. It was found that, CuO and Al₂O₃ doped lithium triborate samples exhibit very significant thermoluminescence glow curves to be promising dosimetric material.     

The existence and the determination of terminal equations for hybrid connected N-ports

A new procedure for the existence and the determination of terminal equations for the hybrid interconnection of two n-port networks is given. It is shown that this procedure generalizes the previous works of Duffin, Trapp and Mitra for the hybrid sum of terminal impedance matrices which are either Hermitian positive semi-definite (HSD) or arbitrary matrices with complex entries. For the application of the new procedure it is shown that each n-port need not be terminal solvable or well defined. In fact, even in the case of active RLC n-ports considered in sinusoidal steady-state, the previous results of Duffin, Mitra and Trapp established for HSD matrices or arbitrary complex matrices can still be utilized with the use of modified terminal equations, to obtain the terminal equations of the resulting n-port networks. It is also noted that the method introduced in this paper is independent of the choice of generalized inverses.     

Evidence of PAH production under lean combustion conditions

While it is common knowledge that PAH formation can be expected at rich combustion conditions, this paper presents experimental and computational evidence that PAH formation can also be expected under lean, or cool, combustion conditions. Experimental evidence for diesel engine operation at lean conditions, as well as the literature references related to waste tire and coal combustion, are cited as supportive evidence of conclusions drawn here. A computational model based on reaction mechanisms for three surrogate fuels: C₂H₂, C₇H₁₆ and C₁₄H₂₈, was validated against the available diesel engine data, and projected to both lean and rich combustion conditions. The finding was that for the adiabatic case, minimum production of PAHs occurs at equivalence of unity and increases as equivalence becomes either lean or rich; the latter observation being consistent with the common knowledge.