Polymeric Nanoparticles as a Self-Adjuvanting Peptide Vaccine Delivery System: The Role of Shape

Antigens incorporated in subunit vaccines are typically poorly immunogenic, so a strong immunostimulant (adjuvant) and/or delivery system is required to boost immunogenicity. In this work, the various functional polymer nanostructures, that is, rods, worms, spheres, and tadpoles are used to develop potent peptide antigen delivery systems. The antigen PADRE-J8 (PJ8), derived from Group A Streptococcus (GAS) M-protein, is either physically mixed or chemically conjugated to polymeric nanoparticles of different shapes. The physical mixture of polymeric nanoparticles and antigen is more effective in inducing antibody production than their chemical conjugates. Moreover, rod-shaped polymeric nanoparticles in physical mixture with PJ8 elicited higher and more opsonic antibody titers than powerful complete Freund's adjuvant (CFA)-adjuvanted antigen. Herein, for the first time it is demonstrated that a) the block copolymer, in nanoparticle form, can act as an immune adjuvant, b) nanoparticle shape plays a crucial role in their immunogenicity, and c) antigen conjugation is not required, nor is antigen encapsulation or absorption.     

Nouvelle expression temporelle de ľimpédance interne des conducteurs à section rectangulaire

In this paper, a new time domain internal impedance formula for characterizing the skin effect in interconnects of rectangular cross section is proposed. The comparison with the simulation results of a method involving frequency domain exact formula validates the present model and illustrates its accuracy. We have also shown the lack of precision of the formulations based on conductor losses varying as √ f In order to predict the responses of lossy planar transmission lines, the used methods are the time domain — frequency domain transformation (tdfd) and the finite difference time domain algorithm (fdtd). Theses techniques of analysis are applied to the mtl equations under quasi — tem approximation.     

SDN-based wireless user management system

The degradation of end-to-end quality of service (QoS) on mobile users is becoming a common issue for IEEE 802.11 infrastructure-based networks in crowded areas. This research tackles the issue by employing an SDN framework on an integrated wireless/wired environment. Thereby, we present the development and implementation of a system that performs user management by analyzing the network load from the OpenFlow statistics, as well as the wireless information collected from the associated users. In order to analyse the behaviour of the proposed user migration algorithm, we evaluate the system under scenarios with different traffic load and user session duration. From the experiments, we observed that in several cases wireless users get a considerable QoS improvement at the application layer (up to 30% improvement in throughput and up to 20% in delay in our simulations) once the system is activated. Based on the results, we present an analysis on how and when user migration in multi-access point IEEE 802.11 networks can be most effective.     

Analyse théorique et caractérisation expérimentale ďune jonction Y intégrée utilisée comme capteur de déplacement

The coherent coupling of radiation modes in symmetric single-mode Y-junction excited by a single mode fiber is studied in this work. Using the bpm (beam propagation method) we find that the power splitting between the two outputs of the junction depends strongly on the fiber displacement with respect to the junction axis of symmetry. A GaAs/GaAlAs single mode junction is tested and the experimental measurements show a splitting ratio as high as 12 dB that could not be explained if the radiation field is neglected. The experimental results confirm the theoretical predictions that take into account the propagation of radiation modes in the structure. These results propose the structure to be used as an integrated optical displacement sensor with a sensitivity in the order of 5 dB/μm and a dynamic range within 4 to 5 μm.     

A novel ±0.5 V,high current drive,and rail to rail current operational amplifier

A current operational amplifier (COA) with very high current drive capability is presented in this paper. The principle of operation of this unique structure is discussed, its most important formulas are derived and its outstanding performance is verified by HSPICE simulation in TSMC 0.18 μm CMOS, BSIM3, and Level49 technology. Owing to the elaborately arranged components, the proposed circuit demonstrates very high frequency bandwidth, extremely high CMRR, high output impedance, and true rail to rail output voltage swing range while operating at very low power supply of ±0.5 V. The interesting results such as current drive capability of ±1 mA, high output impedance of 5 GΩ, wide gain bandwidth of 220 MHz, extremely high output voltage swing of ±0.45 V, which interestingly provides the highest yet reported output voltage compliance for current mode building blocks implemented by regular CMOS technology, low static power consumption of 159 μW, and very high CMRR of 155 dB is achieved utilizing standard CMOS technology. Full process, voltage, and temperature variation analysis of the circuit is also investigated in order to approve the well robustness of the structure. The transient stepwise and sinusoidal response analysis is also done to verify the proposed COA stability.     

Methodologies for characterizing traffic in wide area networks: Analysis and benchmarking

Since computer networks play an important role in distributed computing environments, an application's performance depends heavily on the quality of service provided by the communication networks. To ensure a high performance, the characteristics of wide area networks, WANs, must be well understood. This paper presents methodologies to characterize WAN traffic based on real measurements from Bellcore's backbone network that connects remote sites using dedicated T1 links. This paper also suggests some workload models that can be used for wide area network sizing and performance evaluation studies. It is found that the inter-site traffic pattern depends on the time of the day and the day of the week. Furthermore, the traffic between two sites is found to be reasonably symmetric, except for those sites designated as back-up sites. The coefficient of variation is used as a measure for the traffic burstiness and it is found to be 1·5 degree during working hours. The methods presented here are easy to use and cost-effective.     

A Dual Ring Connectivity Model for VANET Under Heterogeneous Traffic Flow

Vehicular ad-hoc network (VANET) is characterized as a highly dynamic wireless network due to the dynamic connectivity of the network nodes. To achieve better connectivity under such dynamic conditions, an optimal transmission strategy is required to direct the information flow between the nodes. Earlier studies on VANET’s overlook the characteristics of heterogeneity in vehicle types, traffic structure, flow for density estimation, and connectivity observation. In this paper, we have proposed a heterogeneous traffic flow based dual ring connectivity model to enhance both the message disseminations and network connectivity. In our proposed model the availability of different types of vehicles on the road, such as, cars, buses, etc., are introduced in an attempt to propose a new communication structure for moving vehicles in VANETl under cooperative transmission in heterogeneous traffic flow. The model is based on the dual-ring structure that forms the primary and secondary rings of vehicular communication. During message disseminations, Slow speed vehicles (buses) on the secondary ring provide a backup path of communication for high speed vehicles (cars) moving on the primary ring. The Slow speed vehicles act as the intermediate nodes in the aforementioned connectivity model that helps improve the network coverage and end-to-end data delivery. For the evaluation and the implementation of dual-ring model a clustering routing scheme warning energy aware cluster-head is adopted that also caters for the energy optimization. The implemented dual-ring message delivery scheme under the cluster-head based routing technique does show improved network coverage and connectivity dynamics even under the multi-hop communication system.     

Spread spectrum in three-phase inverter by an optimised dual randomised PWM technique

Randomised pulse width modulation (RPWM) technique has become a viable alternative to deterministic pulse width modulation (DPWM). By spreading the power spectrum in a continuous noise, this new technique better complies with electromagnetic compatibility (EMC) requirements for conducted electromagnetic interferences (EMI) and allows reducing the emitted acoustic noise in variable speed drives (VSDs). The most popular RPWM schemes are randomised pulse position modulation (RPPM) and randomised carrier frequency modulation (RCFM). The combination (RCFM-RPPM) or dual RPWM (DRPWM) has also been proposed. In this article, we propose an optimised DRPWM (ODRPWM) for the three-phase inverter. First, the modulating principle is proposed, and then, a mathematical model of power spectral density (PSD) of the output voltage is developed and validated for the three schemes, namely RPPM, RCFM and RCFM-RPPM. PSD analysis shows that the proposed scheme is more effective on spreading PSD. Moreover, this analysis reveals optimal parameters of randomisation for a maximum spread of the PSD. The optimisation problem is then modelled and solved using two powerful non-linear methods.     

Dynamic provisioning of low-speed unicast/multicast traffic demands in mesh-based WDM optical networks

This paper addresses the problem of dynamically provisioning both low-speed unicast and multicast connection requests in mesh-based wavelength division multiplexing (WDM) optical networks. Several routing/provisioning schemes to dynamically provision both unicast and multicast connection requests are presented. In addition, a constraint-based grooming strategy is devised to utilize the overall network resources as efficiently as possible. Based on this strategy, several different sequential multicast grooming heuristics are first presented. Then, we devise a hybrid grooming approach and combine it with sequential approaches to achieve a grooming scheme that is biased toward serving multicast traffic demands in comparison with all other sequential grooming approaches. To achieve our objective, we decompose the problem into four subproblems: 1) routing problem; 2) light-tree-based logical-topology-design problem; 3) provisioning problem; and 4) traffic-grooming problem. The simulation results of the proposed schemes are compared with each other and with those of conventional nongrooming approaches. To the best of our knowledge, this is the first detailed paper to address and examine the problem of grooming dynamic multicast traffic demands.