Inactivation of spores of Bacillus cereus in cheese by high hydrostatic pressure with the addition of nisin or lysozyme

The objective of this work was to study high hydrostatic pressure (HHP) inactivation of spores of Bacillus cereus ATCC 9139 inoculated in model cheeses made of raw milk, together with the effects of the addition of nisin or lysozyme. The concentration of spores in model cheeses was approximately 6-log10 cfu/g of cheese. Cheeses were vacuum packed and stored at 8 degrees C. All samples except controls were submitted to a germination cycle of 60 MPa at 30 degrees C for 210 min, to a vegetative cells destruction cycle of 300 or 400 MPa at 30 degrees C for 15 min, or to both treatments. Bacillus cereus counts were measured 24 h and 15 d after HHP treatment. The combination of both cycles improved the efficiency of the whole treatment. When the second pressure-cycle was of 400 MPa, the highest inactivation (2.4 +/- 0.1 log10 cfu/g) was obtained with the presence of nisin (1.56 mg/L of milk), whereas lysozyme (22.4 mg/L of milk) did not increase sensitivity of the spores to HHP. For nisin (0.05 and 1.56 mg/L of milk), no significant differences were found between counts at 24 h and 15 d after treatment. Considering that mesophilic spore counts usually range from 2.6 to 3.0 log10 cfu/ml in raw milk, HHP at mild temperatures with the addition of nisin may be useful for improving safety and preservation of soft curd cheeses made from raw milk.     

A new vinylpyranoanthocyanin pigment occurring in aged red wine

A new pyranoanthocyanin–vinylphenol pigment was detected in an aged Port red wine. The UV–Vis spectrum of this pigment was found to have a λ_max of 538 nm that is bathochromically shifted from that of original anthocyanins, exhibiting a more purple hue in acidic solution. This newly formed pigment was synthesized in model solution through the reaction between malvidin 3-O-glucoside–pyruvic acid adduct and vinylphenol and its structure was assigned by NMR and mass spectrometry. This pigment is reported herein for the first time.     

Solution of the two-dimensional wave equation by using wave polynomials

The paper demonstrates a specific power-series-expansion technique to solve approximately the two-dimensional wave equation. As solving functions (Trefftz functions) so-called wave polynomials are used. The presented method is useful for a finite body of certain shape geometry. Recurrent formulas for the wave polynomials and their derivatives are obtained in the Cartesian and polar coordinate system. The accuracy of the method is discussed and some examples are shown.     

The impact of wave energy farms in the shoreline wave climate: Portuguese pilot zone case study using Pelamis energy wave devices

This paper describes the study of the impact of energy absorption by wave farms on the nearshore wave climate and, in special, the influence of the incident wave conditions and the number and position of the wave farms, on the nearshore wave characteristics is studied and discussed. The study was applied to the maritime zone at the West coast off Portugal, namely in front of São Pedro de Moel, where it is foreseen the deployment of offshore wave energy prototypes and farms between the 30 m and 90 m bathymetric lines, with an area of 320 Km². In this study the REFDIF model was adapted in order to model the energy extraction by wave farms. Three different sinusoidal incident wave conditions were considered. Five different wave farm configurations, varying the position of the wave farm, its number and the width of the navigation channels at each wave farm were analysed. The results for each configuration in terms of the change of the wave characteristics (wave height and wave direction) at the nearshore are presented, compared and discussed for three representative wave conditions.     

Mo–Si–B alloys for ultra-high-temperature space and ground applications: liquid-assisted fabrication under various temperature and time conditions

Journal of Materials Science - Boron-doped molybdenum silicides have been already recognized as attractive candidates for space and ground ultra-high-temperature applications far beyond limits of...     

Incremental execution of rule-based model transformation

When model transformations are used to implement consistency relations between very large models, incrementality plays a cornerstone role in detecting and resolving inconsistencies efficiently when models are updated. Given a directed consistency relation between two models, the problem studied in this work consists in propagating model changes from a source model to a target model in order to ensure consistency while minimizing computational costs. The mechanism that enforces such consistency is called consistency maintainer and, in this context, its scalability is a required non-functional requirement. State-of-the-art model transformation engines with support for incrementality normally rely on an observer pattern for linking model changes, also known as deltas, to the application of model transformation rules, in so-called dependencies, at run time. These model changes can then be propagated along an already executed model transformation. Only a few approaches to model transformation provide domain-specific languages for representing and storing model changes in order to enable their use in asynchronous, event-based execution environments. The principal contribution of this work is the design of a forward change propagation mechanism for incremental execution of model transformations, which decouples dependency tracking from change propagation using two innovations. First, the observer pattern-based model is replaced with dependency injection, decoupling domain models from consistency maintainers. Second, a standardized representation of model changes is reused, enabling interoperability with EMF-compliant tools, both for defining model changes and for processing them asynchronously. This procedure has been implemented in a model transformation engine, whose performance has been evaluated experimentally using the VIATRA CPS benchmark. In the experiments performed, the new transformation engine shows gains in the form of several orders of magnitude in the initial phase of the incremental execution of the benchmark model transformation and change propagation is performed in real time for those model sizes that are processable by other tools and, in addition, is able to process much larger models.

     

Safe reuse in modelling language engineering using model subtyping with OCL constraints

Software and Systems Modeling - Low-code software development promises rapid delivery of software cloud applications by employing domain-specific languages (DSLs), requiring minimal traditional...     

Deep Drawing of Friction Stir Welded Thin Sheet Aluminium Steel Tailored Hybrids

Friction stir welding undergoes a steep evolution in industrial applications since the invention in the early 1990s. Especially for aluminium alloys in sheet thicknesses over 2 mm a lot of applications are established, whereas a lack in knowledge about friction stir welding of thin sheets with sheet thickness less than 2 mm exists. This article deals with friction stir welding of thin sheet aluminium steel tailored hybrids and their formability. These investigations tend to close the gap of availability of friction stir welded blanks in the range of 1 mm sheet thickness and to offer new applications of this joining technology. For production of aluminium steel tailored hybrids AA5182 with a thickness of 1.2 mm and DC04 in 1.0 mm are used, the joining partners are friction stir welded in a lap joint. Different tool geometries and process parameters are performed to achieve the highest strength and elongation at fracture of the tailored hybrids. The influence of the stirring on the arrangement and distribution of both materials in the welding zone and its microstructure is analysed using light optical and scanning electron microscopy. In addition to tensile tests planar microhardness measurements help to detect the local changes of the mechanical properties in the characteristic zones of the weld seam. Tailored hybrids, which were friction stir welded with the best welding parameters in accordance to the mechanical properties of the weld seams, were used for deep drawing tests of friction stir welded thin sheet aluminium steel tailored hybrids. The maximum drawing ratio of these tailored hybrids coincides with the one of the parent material of AA5182.     

Design of whey protein nanostructures for incorporation and release of nutraceutical compounds in food

Whey proteins are widely used as nutritional and functional ingredients in formulated foods because they are relatively inexpensive, generally recognized as safe (GRAS) ingredient, and possess important biological, physical, and chemical functionalities. Denaturation and aggregation behavior of these proteins is of particular relevance toward manufacture of novel nanostructures with a number of potential uses. When these processes are properly engineered and controlled, whey proteins may be formed into nanohydrogels, nanofibrils, or nanotubes and be used as carrier of bioactive compounds. This review intends to discuss the latest understandings of nanoscale phenomena of whey protein denaturation and aggregation that may contribute for the design of protein nanostructures. Whey protein aggregation and gelation pathways under different processing and environmental conditions such as microwave heating, high voltage, and moderate electrical fields, high pressure, temperature, pH, and ionic strength were critically assessed. Moreover, several potential applications of nanohydrogels, nanofibrils, and nanotubes for controlled release of nutraceutical compounds (e.g. probiotics, vitamins, antioxidants, and peptides) were also included. Controlling the size of protein networks at nanoscale through application of different processing and environmental conditions can open perspectives for development of nanostructures with new or improved functionalities for incorporation and release of nutraceuticals in food matrices.     

Medium frequency magnetron self-sputtering of copper

It is well known that the magnetron self-sustained sputtering (SSS) process can be achieved in the direct current (DC) operation mode (DC-SSS) if certain conditions are fulfilled: high self-sputtering yield of the target material (theoretically Y>1), appropriate magnetron source design, high target power density to ensure high ionization level of the sputtered material. The main disadvantage of the DC-SSS process is the instability related to possibility of an arc formation. The author postulates that magnetron plasma pulsing can minimize this problem.In this paper, voltage and current waveforms of medium frequency (MF) powered magnetron source are analysed. A simple electrical model, explaining dynamics of MF magnetron discharge is presented. The method to achieve MF magnetron SSS process is proposed and experimentally verified. The results of MF magnetron SSS (MF-SSS) process are presented for the first time (to the author's knowledge). The experiments were performed using a planar magnetron source equipped with a copper target of 50 mm in diameter and 6 mm thick. The magnetron source was powered by a resonant (110 kHz) power supply. The target power density during MF-SSS process was about 490 W/cm².