Preparation of high-modulus and high-strength poly(ethylene terephthalate) film by zone-annealing method

The zone-annealing method was attempted to prepare high-modulus and high-strength poly(ethylene terephthalate) (PET) film. The film having Young′s modulus of 14.5 × 10¹⁰ dyn/cm² and a tensile strength of 86.9 kg/mm² in the drawing direction was obtained. These values correspond to four times those of a biaxial-stretched PET film available commercially. The dyanmic viscoelastic properties also were measured. The dynamic storage modulus was 15.4 × 10¹⁰ dyn/cm² at room temperature, and a high value of 3.6 × 10¹⁰ dyn/cm² even at 200°C. The latter value is slightly higher than the room temperature modulus of the commercially available film. From the intensity and temperature position of α-dispersion E″ peak, it was suggested that the amorphous chains in the zone-annealed film was densely packed in a highly oriented state. Furthermore, the crystallinity, orientation, especially double orientation, and molecular chain conformation of the films in the zone-annealing process are discussed in the present paper.     

Cytocompatibility of magnesium and AZ31 alloy with three types of cell lines using a direct in vitro method

Magnesium alloys have been investigated by many researchers as a new absorbable biomaterial owing to their excellent degradability with non-maleficence or low-maleficence in living tissues. In the present work, the in vitro cytocompatibility of an Magnesium alloy was investigated by culturing cells directly on it. Investigations were carried out in terms of the cell viability along with the use of scanning electron microscopy to observe its morphology. The cell lines used were derived from fibroblast, endothelial, and smooth muscle cells. Pure magnesium and AZ31 alloy composed of magnesium (96?%), aluminum (3?%), and zinc (1?%) were adopted as models. The viability of cells on the metal samples and on the margin area of a multi-well plate was investigated. For direct culturing on metal, a depression in the viability and morphologically stressed cells were observed. In addition, the cell viability was also depressed for the margin area. To clarify the factors causing the negative effects, the amount of eluted metal ions and pH changes in the medium because of the erosion of the Magnesium samples were investigated, together with the cytotoxicity of sole metal ions corresponding to the composition of the metals. It was found that Mg²⁺, Zn²⁺, and Al³⁺ ions were less toxic at the investigated concentrations, and that these factors will not produce negative effects on cells. Consequently, these factors cannot fully explain the results.     

NMR STUDY OF LANTHANIDE(III) NITRATE-CMPO EXTRACTION SYSTEM (I) STRUCTURE OF EXTRACTEDCHEMICAL SPECIES

ABSTRACT

Octyl(phenyl)-N,N-dtisobutylcarbamoylmethylphosphine oxide used in the TRUEX process is a new extractant for TRU elements. NMR measurements were carried out to study the coordination structure of lanthanide(III)-CMPO complexes. By analyzing Lan- thanide-Induced-Shifts(LIS) data It was found that CMPO molecule locates in the equatorial region with respect to the principal magnetic Z axis. From the NMR longitudinal relaxation time measurements for Ce(N0₃)₃· 3CMPO, the distance of Ce³⁺-P, Ce³⁺- C(bridging methylene carbon) and Ce³⁺-C(carbonyl carbon) were estimated to be 3.3, 3.1 and 3.1 Å, respectively, suggesting that CMPO coordinates to the central Ce³⁺ ion in a bidentate manner with the phosphoryl and carbonyl groups. This is consistent with the low frequency shifts of phosphoryl and carbonyl stretching frequencies observed in the IR spectrum of Ce(N0₃)₃·3CMPO. Moreover, from the dependence of ³¹P-NMR spectra for Nd(N0₃)₃·3CMPO and Er(N0₃)₃·2CMPO on temperature, lanthanide(IH) -CMPO complexes exist as several isomers in CDC1₃ and a rapid intramolecular interconversion occurs at higher temperature region.     

Plasma Sphingosine-1-Phosphate Levels Are Associated with Progression of Estrogen Receptor-Positive Breast Cancer

Although numerous experiments revealed an essential role of a lipid mediator, sphingosine-1-phosphate (S1P), in breast cancer (BC) progression, the clinical significance of S1P remains unclear due to the difficulty of measuring lipids in patients. The aim of this study was to determine the plasma concentration of S1P in estrogen receptor (ER)-positive BC patients, as well as to investigate its clinical significance. We further explored the possibility of a treatment strategy targeting S1P in ER-positive BC patients by examining the effect of FTY720, a functional antagonist of S1P receptors, on hormone therapy-resistant cells. Plasma S1P levels were significantly higher in patients negative for progesterone receptor (PgR) expression than in those positive for expression (p = 0.003). Plasma S1P levels were also significantly higher in patients with larger tumor size (p = 0.012), lymph node metastasis (p = 0.014), and advanced cancer stage (p = 0.003), suggesting that higher levels of plasma S1P are associated with cancer progression. FTY720 suppressed the viability of not only wildtype MCF-7 cells, but also hormone therapy-resistant MCF-7 cells. Targeting S1P signaling in ER-positive BC appears to be a possible new treatment strategy, even for hormone therapy-resistant patients.     

Shape optimization of one‐chamber perforated plug/non‐plug mufflers by simulated annealing method

To economically and efficiently lower the venting noise, the development of a high‐quality muffler with compact volume has become crucial in the modern industrial field. The research work of shape optimization of straight silencers in conjunction with plug/non‐plug perforated ducts which may noticeably increase the acoustical performance is rarely addressed; therefore, the main purpose of this paper is not only to analyze the sound transmission loss (STL) of a one‐chamber plug/non‐plug perforated muffler but also to optimize the best design shape under a limited space. In this paper, on the basis of plane wave theory, the four‐pole system matrix in evaluating the acoustic performance is derived by using the decoupled numerical method. Moreover, a simulated annealing (SA) algorithm searching for the global optimum by imitating the softening process of metal has been adopted during the muffler's optimization. To assure SA's correctness, the STL's maximization of one‐chamber perforated plug mufflers at a targeted frequency of 500 Hz is exemplified first. Furthermore, a numerical case in dealing with a broadband noise emitted from a fan by using one‐chamber plug/non‐plug mufflers has been introduced and fully discussed. To achieve a better optimization in SA, various SA parameter sets of cooling rate and iteration parameter values were used. Before the SA operation can be carried out, the accuracy check of the mathematical models with respect to plug/non‐plug perforated mufflers has to be supported by experimental data. The optimal result in eliminating broadband noise reveals that the muffler with a plug acoustical mechanism has a better noise reduction than that of a non‐plug muffler. Consequently, the approach used for the optimal design of the noise elimination proposed in this study is certainly easy, economical, and quite effective. Copyright © 2007 John Wiley & Sons, Ltd.     

Intron-Dependent or Independent Pseudouridylation of Precursor tRNA Containing Atypical Introns in Cyanidioschyzon merolae

Eukaryotic precursor tRNAs (pre-tRNAs) often have an intron between positions 37 and 38 of the anticodon loop. However, atypical introns are found in some eukaryotes and archaea. In an early-diverged red alga Cyanidioschyzon merolae, the tRNA^Ile(UAU) gene contains three intron coding regions, located in the D-, anticodon, and T-arms. In this study, we focused on the relationship between the intron removal and formation of pseudouridine (Ψ), one of the most universally modified nucleosides. It had been reported that yeast Pus1 is a multiple-site-specific enzyme that synthesizes Ψ34 and Ψ36 in tRNA^Ile(UAU) in an intron-dependent manner. Unexpectedly, our biochemical experiments showed that the C. merolae ortholog of Pus1 pseudouridylated an intronless tRNA^Ile(UAU) and that the modification position was determined to be 55 which is the target of Pus4 but not Pus1 in yeast. Furthermore, unlike yeast Pus1, cmPus1 mediates Ψ modification at positions 34, 36, and/or 55 only in some specific intron-containing pre-tRNA^Ile(UAU) variants. cmPus4 was confirmed to be a single-site-specific enzyme that only converts U55 to Ψ, in a similar manner to yeast Pus4. cmPus4 did not catalyze the pseudouridine formation in pre-tRNAs containing an intron in the T-arm.     

Assessment of polyester and polyvinylester coatings in a simulated FGD scrubbing liquid with EIS

Abstract

Corrosion behaviours of carbon steel coated with polyester and polyvinylester in a simulated flue gas desulphurisation (FGD) fluid (i.e., a mixture of H₂SO₄ and 0.1 wt% Fe₂(SO₄)₃ at pH=2) have been investigated with electrochemical impedance spectroscopy (EIS). The protective nature of the coatings at 25°C has been observed in the following order: polyvinylester primer >> polyvinylester topcoat > polyester topcoat > polyester primer. Coating deterioration occurred and the coating deterioration rate accelerated with increasing temperatures upto 85°C. However, the corrosion resistance sequence has remained the same. Equivalent circuits are suggested for various coatings to discuss their mechanisms in this work.     

Remote calibration of ionization chambers for radioactivity calibration

A new calibration technique, referred to as e-trace, has been developed by the National Institute of Advanced Industrial Science and Technology (AIST). The e-trace technique enables rapid remote calibration of measurement equipment and requires minimal resources. We calibrated radioisotope calibrators of the Japan Radioisotope Association (JRIA) and the Nishina Memorial Cyclotron Center (NMCC) remotely and confirmed that remote calibration provided results that are consistent with the results obtained by existing methods within the limits of uncertainty. Accordingly, e-trace has been approved as the standard calibration method at AIST. We intend to apply remote calibration to radioisotope calibrators in hospitals and isotope facilities.     

All solid-state lithium polymer secondary batteries using spinel Li4/3Ti5/3O4 as an active material

Present paper describes electrochemical performance of the all solid-state lithium polymer battery (LBP) using spinel-type Li_4/3Ti_5/3O₄ which has been known as the potential candidate of anode materials.The assembled LPB with Li|solid polymer electrolyte(SPE)|Li_4/3Ti_5/3O₄ construction showed stable charge-discharge cycles more than 300 times at 1 C condition. On the other hand, strong charge-discharge rate dependence for the specific capacity and initial capacity loss was indicated. Such a poor rate performance stemmed from low diffusivity of Li⁺ ion in the by-products produced by the decomposition of SPE components at the SPE|Li_4/3Ti_5/3O₄ interface.