Characterization,Expression Profiling,and Biochemical Analyses of the Cinnamoyl-CoA Reductase Gene Family for Lignin Synthesis in Alfalfa Plants

Cinnamoyl-CoA reductase (CCR) is a pivotal enzyme in plant lignin synthesis, which has a role in plant secondary cell wall development and environmental stress defense. Alfalfa is a predominant legume forage with excellent quality, but the lignin content negatively affects fodder digestibility. Currently, there is limited information on CCR characteristics, gene expression, and its role in lignin metabolism in alfalfa. In this study, we identified 30 members in the CCR gene family of Medicago sativa. In addition, gene structure, conserved motif, and evolution analysis suggested MsCCR1–7 presumably functioned as CCR, while the 23 MsCCR-likes fell into three categories. The expression patterns of MsCCRs/MsCCR-likes suggested their role in plant development, response to environmental stresses, and phytohormone treatment. These results were consistent with the cis-elements in their promoters. Histochemical staining showed that lignin accumulation gradually deepened with the development, which was consistent with gene expression results. Furthermore, recombinant MsCCR1 and MsCCR-like1 were purified and the kinetic parameters were tested under four substrates. In addition, three-dimensional structure models of MsCCR1 and MsCCR-like1 proteins showed the difference in the substrate-binding motif H212(X)2K215R263. These results will be useful for further application for legume forage quality modification and biofuels industry engineering in the future.     

大型风力发电场并网接入运行问题综述

指出风电场与常规能源电厂的主要区别,介绍了恒速恒频和变速恒频两种风力发电系统的运行原理及各自接入电力系统的现状和主要问题,阐述了风力发电场并网过程时电网产生冲出,风电场运行对电能质量、保护装置、电网频率和对电力系统运行成本等几方面产生的影响.     

FNN与混合遗传算法在智能优化的策略比较

FNN是一种重要的神经网络结构,混合遗传算法是一种正在兴起的启发式随机搜索技术,两者在智能优化中得到了广泛的应用。两者的优化机理不同,方法不同,各有千秋。论文从提高两者的优化性能出发,研究其智能优化的实现策略,并对两者进行了比较,得出了有意义的结论。     

双足足球机器人守门策略研究

首先提出了双足足球机器人的体系结构,然后给出了基本步态算法,针对FIRA类人型项目比赛规则,提出了全新的双足足球机器人守门策略,最后给出了分析测距试验数据,验证了测距准确性和守门策略的合理性.     

多功能Pt-Ce6纳米平台作为过氧化氢纳米酶和NIR-Ⅱ光热剂用于增强PDT/PTT肿瘤治疗

实体肿瘤的缺氧严重影响着基于氧气的光动力疗法(PDT)的效果.另外,单一治疗模式通常难以达到满意的治疗效果.为此,我们设计合成了一种多功能纳米复合材料Pt-Ce6用于克服肿瘤乏氧,实现PDT/PTT协同治疗.在该体系中,我们使用多孔Pt纳米粒子作为过氧化氢纳米酶、近红外二区(NIR-Ⅱ)光热转换剂和光敏剂二氢卟吩e6(...     

Preparation and properties of magnesium oxysulfate cement and its application as lost circulation materials

Loss of drilling fluids in large porous and fractured zones inevitably up-regulates the overall cost of drilling.As a type of acid-soluble cement,magnesium oxys...     

Research on the sticking-sliding contact ratio in high-speed cutting of cupronickel B10

Journal of Mechanical Science and Technology - Accurate understanding of the frictional behavior at the tool-chip interface is critical for the cutting process. To quantitatively analyze the ratio...     

抗震高可靠电子枪的设计

电视跟踪、电视制导是导弹武器系统的重要组成部份,摄象器件又是其中的关键器件。本文着重从抗震、高可靠方面介绍摄象器件电子枪的结构和性能设计,该电子枪应用于重点军事工程,经多次试验和实际使用,性能稳定,达到了高可靠的要求,是目前国内唯一的抗震高可靠电子枪。     

自动送粉激光熔覆装置及工艺研究

研制了一种工作稳定性好、粉末流量连续可调的自动送粉装置。经A_3钢表面激光熔覆WF-150不锈钢合金粉末的研究表明,所获得的激光熔覆层均匀连续、无宏观气孔和裂纹,粉末利用率高达94％。     

纳米酶在食品保鲜中的应用

具有优异广谱抗菌特性的纳米酶,为解决由微生物引起的食品腐败问题提供了一种全新策略。纳米酶可在生理环境中催化相应的底物而产生活性氧,因其制备简便、成本低、催化效率高,易于规模化生产和稳定性高等优点,故在食品保鲜领域具有广阔的应用前景。本文综述纳米酶的分类,包括金属或金属氧化物基纳米酶、碳基纳米酶、聚合物基纳米酶及其它材料,调控纳米酶抗菌活性的主要因素(大小、形态、涂层及改性、组成成分等),为如何设计高效抗菌纳米酶提供了参考。本文还阐述纳米酶在食品保鲜中的应用,包括纳米酶基抗菌薄膜应用及其在检测食品中细菌污染水平中的应用。由于其广谱抗菌特性和可忽略的生物毒性,抗菌纳米酶能降低食品工业中微生物的危害,而且有助于控制食品生产及加工中的交叉污染问题,对保障食品安全,促进食品工业健康可持续发展具有十分重要的现实意义。总而言之,抗菌纳米酶有望成为一种新型的抗菌剂和/或策略,在食品保鲜中展现出广阔的应用前景。