Immunohistochemical and lectin histochemical studies on the developing olfactory organs of fetal camel

Little is known about the development of the olfactory organs of camel. In this study, prenatal development and neuronal differentiation of the vomeronasal organ (VNO) and the olfactory epithelium (OE) of the one‐humped camel were studied by immunohistochemistry and lectin histochemistry. A neuronal marker, protein gene product (PGP) 9.5, but not a marker of fully differentiated olfactory receptor cells, olfactory marker protein, intensely labeled the olfactory receptor cells of the VNO and OE at 395 mm, 510 mm, and 530 mm fetal ages, indicating that the olfactory receptor cells are differentiated, but not fully matured both in the VNO and the OE. In 187 mm and 190 mm fetuses, PGP 9.5 yielded faint immunoreactive signals in the VNO, but not in the OE, although the presence of olfactory receptor cells were demonstrated in both tissues by intense WGA and LEL stainings. We conclude that the camel VNO and OE bear differentiated, but still immature receptor cells; in addition, the onset of neuronal differentiation seems to be somewhat earlier in the VNO than in the OE till half of the prenatal life. Microsc. Res. Tech. 78:613–619, 2015. © 2015 Wiley Periodicals, Inc.     

Effects of gamma irradiation on the viability and phenotypic characteristics of Salmonella Enteritidis inoculated into specific-pathogen-free eggs

The goal of this study was to determine the effects of various levels of gamma irradiation on the phenotypic characteristics of 20 strains of Salmonella Enteritidis inoculated separately into specific-pathogen-free shell eggs. Bacterial strains were inoculated into egg yolks and exposed to (60)Co radiation at doses of 0.49 to 5.0 kGy. The eggs were maintained at 25°C and analyzed for the presence of Salmonella on days 1, 2, 4, and 7, and the recovered Salmonella isolates were characterized biochemically. All strains were resistant to doses of 0.49, 0.54, 0.59, 0.8, and 1 kGy; colony counts were ≥10(5) CFU/ml of egg yolk except for one strain, which was detected at 96 h and at 7 days after irradiation at 1 kGy, with a population reduction of 2 log CFU/ml. For the other evaluated doses, 12 strains (60.0%) were resistant at 1.5 kGy and 7 strains (35.0%) were resistant at 3.0 kGy. Among all analyzed strains, 5.0 kGy was more effective for reducing and/or eliminating the inoculated bacteria; only two (10%) strains were resistant to this level of irradiation. Salmonella colony counts were significantly reduced (P < 0.01) with increasing doses from the day 1 to 7 of observation, when microbial growth peaked. Loss of mobility, lactose fermentation, citrate utilization, and hydrogen sulfide production occurred in some strains after irradiation independent of dose and postirradiation storage time. Increases in antibiotic susceptibility also occurred: seven strains became sensitive to β-lactams, two strains became sensitive to antifolates, and one strain each became sensitive to fluoroquinolone, phenicol, nitrofurans, tetracyclines, and aminoglycosides. The results indicate that up to 5.0 kGy of radiation applied to shell eggs inoculated with Salmonella Enteritidis at 4 log CFU per egg is not sufficient for complete elimination of this pathogen from this food matrix.     

Moderating effect of social support on the stress–burnout relationship.

Assessed the extent of life and work stresses, the availability of social support, and experience of burnout through a self-report questionnaire administered to 357 female (mean age 32 yrs) and 273 male (mean age 39 yrs) Israeli managers and human service professionals. Analysis showed that female Ss experienced more burnout and stress in life than did male Ss; no difference was found between the sexes for stress in work. Burnout was positively correlated with stress and negatively correlated with social support in both life and work. Further analysis revealed different patterns of moderating effects of social support on the relationship between stress and burnout for males and females: The relationship between work stress and burnout was moderated by support in life for females and by support in work for males. It is concluded that special attention should be paid to cultural, occupational, and sex differences in social support research and the exploration of its buffering effect. (47 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

SEPARATION OF THE CARRIER FREE RADIOISOTOPES OF LANTHANIDE SERIES ELEMENTS

ABSTRACT

The various methods of separation of carrier free lanthanide elements have been briefly described.     

Oxidative and Molecular Responses in Capsicum annuum L. after Hydrogen Peroxide,Salicylic Acid and Chitosan Foliar Applications

Hydrogen peroxide (H₂O₂) is an important ROS molecule (Reactive oxygen species) that serves as a signal of oxidative stress and activation of signaling cascades as a result of the early response of the plant to biotic stress. This response can also be generated with the application of elicitors, stable molecules that induce the activation of transduction cascades and hormonal pathways, which trigger induced resistance to environmental stress. In this work, we evaluated the endogenous H₂O₂ production caused by salicylic acid (SA), chitosan (QN), and H₂O₂ elicitors in Capsicum annuum L. Hydrogen peroxide production after elicitation, catalase (CAT) and phenylalanine ammonia lyase (PAL) activities, as well as gene expression analysis of cat1, pal, and pathogenesis-related protein 1 (pr1) were determined. Our results displayed that 6.7 and 10 mM SA concentrations, and, 14 and 18 mM H₂O₂ concentrations, induced an endogenous H₂O₂ and gene expression. QN treatments induced the same responses in lesser proportion than the other two elicitors. Endogenous H₂O₂ production monitored during several days, showed results that could be an indicator for determining application opportunity uses in agriculture for maintaining plant alert systems against a stress.     

Energy-Efficient Routing Using Novel Optimization with Tabu Techniques for Wireless Sensor Network

Wireless Sensor Network (WSN) consists of a group of limited energy source sensors that are installed in a particular region to collect data from the environment. Designing the energy-efficient data collection methods in large-scale wireless sensor networks is considered to be a difficult area in the research. Sensor node clustering is a popular approach for WSN. Moreover, the sensor nodes are grouped to form clusters in a cluster-based WSN environment. The battery performance of the sensor nodes is likewise constrained. As a result, the energy efficiency of WSNs is critical. In specific, the energy usage is influenced by the loads on the sensor node as well as it ranges from the Base Station (BS). Therefore, energy efficiency and load balancing are very essential in WSN. In the proposed method, a novel Grey Wolf Improved Particle Swarm Optimization with Tabu Search Techniques (GW-IPSO-TS) was used. The selection of Cluster Heads (CHs) and routing path of every CH from the base station is enhanced by the proposed method. It provides the best routing path and increases the lifetime and energy efficiency of the network. End-to-end delay and packet loss rate have also been improved. The proposed GW-IPSO-TS method enhances the evaluation of alive nodes, dead nodes, network survival index, convergence rate, and standard deviation of sensor nodes. Compared to the existing algorithms, the proposed method outperforms better and improves the lifetime of the network.     

Nanoarchitectonics of Hydroxyapatite/Molybdenum Trioxide/Graphene Oxide Composite for Efficient Antibacterial Activity

Nanocomposites based on hydroxyapatite (HAP), including MoO₃, HAP/MoO₃, HAP/GO and HAP/MoO₃/GO have been studied to be suggested for biological usage. The different compositions were investigated using FTIR, XRD, EDS, and XPS analysis. The tight relation between morphological features and composites' chemical ingredients was also studied. According to TEM micrographs, it was mentioned that the disappearance of well-defined grains after the combinations of HAP and MoO₃, while graphene oxide (GO) caused a reduction in size and maintaining the particles’ shape. The combination between HAP/MoO₃ declines the roughness of both HAP and MoO₃ individually, recording 27.5 nm, while HAP/GO and HAP/MoO3/GO exhibit in-between roughness average (Ra) value among its raw constituents with 34.7 and 33.5 nm, respectively. Furthermore, SEM micrographs and roughness results show how to tailing the proper features for the proposed application by changing the type and amount of additives into HAP. Thus, the composite (HAP/MoO₃/GO) displays the uppermost cell viability compared with the rest compositions with 97.8?±?3.0%. Additionally, this triple composite hits the peak germicidal behavior with 17.9?±?1.2 and 16.5?±?0.9 mm against both E.coli and S.aureus, respectively.

     

Flow of mixed convection for radiative and magnetic hybrid nanofluid in a porous material with Joule heating

This paper analyzes the mixed convection flow and transport of heat in a hybrid nanofluid via an exponentially extending/contracting surface. Joule heating, magnetic field, permeability of a porous medium, thermal radiation, and slip condition are taken into consideration. Magnetite (Fe₃O₄) and copper (Cu) are used as a mixture of nanoparticles while ethylene glycol as a regular liquid. The paradigm is dissolved by utilizing the method of Runge–Kutta–Fehlberg with the shooting technique in MATLAB software. The effect of controlling parameters on the coefficient of drag force, heat transfer coefficient, and the distributions of temperature and velocity for physical parameters are discussed numerically, physically, and graphically. The outcomes ended up illustrating that the transport of heat is diminished by upsurging the Joule heating and magnetic field parameters for both contracting and extending states. For larger values of permeability parameter and parameter of mixed convection, the coefficient of local skin friction upsurges in extending situations.     

Rational Design and Synthesis of Selective PRMT4 Inhibitors: A New Chemotype for Development of Cancer Therapeutics**

Protein arginine N-methyl transferase 4 (PRMT4) asymmetrically dimethylates the arginine residues of histone H3 and nonhistone proteins. The overexpression of PRMT4 in several cancers has stimulated interest in the discovery of inhibitors as biological tools and, potentially, therapeutics. Although several PRMT4 inhibitors have been reported, most display poor selectivity against other members of the PRMT family of methyl transferases. Herein, we report the structure-based design of a new class of alanine-containing 3-arylindoles as potent and selective PRMT4 inhibitors, and describe key structure–activity relationships for this class of compounds.