Optimum lipid production using agro-industrial wastewater treated microalgae as biofuel substrate

Abundant wastewater discharges from palm oil industries in tropical nations being a valuable resource of biodiesel need proper exploration. Research hinted that such wastewater as economical nutrient source or substrate can support the cultivation of microalgae. In this experiment, we have tested the growth and lipid production of five different microalgal strains in palm oil mill effluent (POME). POME as a biofuel substrate is demonstrated to be lucrative for microalgae-assisted lipids production. POME is rich in macro- and micronutrients can be used as a growth medium for algal growth in order to reduce the growth medium cost and environmental pollutions. Among the five microalgal strains tested, Chlorella sorokiniana revealed optimum biomass and lipid production. The productivity was evaluated in terms of chlorophyll content, growth rate, biomass, and lipid content, which discerned to be 0.099/day, 8.0 mg/L day and 2.68 mg/mg cell dry weight (CDW). Furthermore, in this study, an optimization study was carried out to enhance the microalgae to produce high lipid content using carbon-to-nitrogen ratio and different light/dark periods. The presence of nitrogen combined glucose (with a carbon-to-nitrogen ratio 100:7) as an alternative source to carbon displayed higher lipid production of 2.68 (mg/mg CDW) by C. sorokiniana. This study confirms that 8:16 h light/dark condition at C:TN ratio of 100:7 supported to produce high lipid content of 17 mg lipid/mg CDW. The above results revealed that POME could be a suitable growth media for the alga C. sorokiniana to improve the maximum lipid yield for biofuels production.     

Review: the latest advances in biomedical applications of chitosan hydrogel as a powerful natural structure with eye-catching biological properties

Journal of Materials Science - Chitosan is one of the natural cationic polymers with unique properties such as non-toxicity, biodegradability, biocompatibility, environmentally friendly that has...     

Advances in Antimicrobial Microneedle Patches for Combating Infections

Skin infections caused by bacteria, viruses and fungi are difficult to treat by conventional topical administration because of poor drug penetration across the stratum corneum. This results in low bioavailability of drugs to the infection site, as well as the lack of prolonged release. Emerging antimicrobial transdermal and ocular microneedle patches have become promising medical devices for the delivery of various antibacterial, antifungal, and antiviral therapeutics. In the present review, skin anatomy and its barriers along with skin infection are discussed. Potential strategies for designing antimicrobial microneedles and their targeted therapy are outlined. Finally, biosensing microneedle patches associated with personalized drug therapy and selective toxicity toward specific microbial species are discussed.     

Characterisation of CO/NO/SO2 emission and ash-forming elements from the combustion and pyrolysis process

Bioenergy is considered as a sustainable energy which can play a significant role in the future’s energy scenarios to replace fossil fuels, not only in the heat production, but also in the electricity and transportation sectors. Emission formation and release of main ash-forming elements during thermal conversion of biomass fuels at different conditions have been the scope of this study. The experiments were conducted in a quartz glass reactor where the temperature and atmosphere could be controlled. The selected fuels represent a wide range of biomass compositions. They are torrefied softwood, spruce bark, waste wood, miscanthus, and wheat straw. The fuels were first grinded and then pressed with a pellet maker into pellets of the same size and weight. For each fuel, the experiments were carried out under both oxidation and pyrolysis condition, with atmosphere of 3 % O₂ + 97 % N₂ and 100 % N₂, respectively, at four residence times. The selected temperatures under which experiments were performed are 800, 900, and 1,050 °C. The concentration of SO₂, NO, CO, and CO₂ emissions and O₂ were monitored online by three analysers, simultaneously. The residue weight was measured after each process, and the comparison with the ash content of the fresh pellet is made. Additionally, the release of several ash-forming elements (K, Zn, Na, and Mn) from the fuels has been quantified as function of temperature and residence time by inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma atomic emission spectroscopy (ICP-AES). Time-dependent formation of NO and SO₂ and other emissions is presented and discussed with respect to different temperature and combustion conditions.     

Iron oxide/gold nanoparticles‐decorated reduced graphene oxide nanohybrid as the thermo‐radiotherapy agent

The main focus of the current study is the fabrication of a multifunctional nanohybrid based on graphene oxide (GO)/iron oxide/gold nanoparticles (NPs) as the combinatorial cancer treatment agent. Gold and iron oxide NPs formed on the GONPs via the in situ synthesis approach. The characterisations showed that gold and iron oxide NPs formed onto the GO. Cell toxicity assessment revealed that the fabricated nanohybrid exhibited negligible toxicity against MCF‐7 cells in low doses (<50 ppm). Temperature measurement showed a time and dose‐dependent heat elevation under the interaction of the nanohybrid with the radio frequency (RF) wave. The highest temperature was recorded using 200 ppm concentration nanohybrid during 40 min exposure. The combinatorial treatments demonstrated that the maximum cell death (average of 53%) was induced with the combination of the nanohybrid with RF waves and radiotherapy (RT). The mechanistic study using the flow cytometry technique illustrated that early apoptosis was the main underlying cell death. Moreover, the dose enhancement factor of 1.63 and 2.63 were obtained from RT and RF, respectively. To sum up, the authors’ findings indicated that the prepared nanohybrid could be considered as multifunctional and combinatorial cancer therapy agents.Inspec keywords: radiation therapy, toxicology, gold, biomedical materials, nanofabrication, nanoparticles, iron compounds, cancer, nanomedicine, cellular biophysics, tumours, graphene compounds, biothermicsOther keywords: graphene oxide nanohybrid, combinatorial cancer treatment agent, cell toxicity assessment, MCF‐7 cells, dose‐dependent heat elevation, multifunctional cancer therapy agents, thermoradiotherapy agent, graphene oxide‐iron oxide‐gold nanoparticles, temperature measurement, radiofrequency wave, flow cytometry, time 40.0 min, CO‐FeO‐Au     

Putative mechanism for anticancer properties of Ag–PP (NPs) extract

One of the most important challenges in treating cancer is the invasion and the angiogenesis of cancer cells. The synthesis of green nanoparticles (NPs) and their use in therapeutic fields is one of the most effective methods with minimal side effects in cancer treatment. In this study, cytotoxic and anti‐angiogenic effects of silver NPs (AgNPs) coated with palm pollen extract [Ag–PP(NPs)] were evaluated. For this purpose, the cells were treated with NPs and then were subjected to trypan blue testing (48 h). Then, the cancer invasion was evaluated by the scratch procedure and the expressions of the vascular endothelial growth factor (VEGF) and its receptor (VEGF‐R) genes were estimated using real‐time PCR assay. Also, the angiogenesis effect of the NPs was investigated with chick chorioallantoic membrane (CAM) assay. The Ag–PP(NPs) induced cytotoxicity on MCF7 cells. The findings also showed that Ag–PP(NPs) inhibit invasive cancer cells and reduce the expression of VEGF and VEGF‐R and significantly reduced the number and vessels lengths and the lengths and weights of the embryos in CAM assay. Ag–PP(NPs) with the induction of cytotoxic effects, metastatic inhibition and anti‐angiogenesis properties should be considered as an appropriate option for treatment of cancerInspec keywords: nanomedicine, genetics, cellular biophysics, toxicology, patient treatment, silver, cancer, biochemistry, biomedical materials, nanoparticles, nanofabrication, membranesOther keywords: minimal side effects, cancer treatment, silver NPs, cancer invasion, vascular endothelial growth factor, receptor genes, VEGF‐R, real‐time polymerase chain reaction assay, angiogenesis effect, chick chorioallantoic membrane assay, MCF7 cells, invasive cancer cells, cytotoxic effects, putative mechanism, anticancer properties, antiangiogenic effects, antiangiogenesis properties, Ag–PP‐induced cytotoxicity, metastatic inhibition, palm pollen extraction, trypan blue testing, time 48.0 hour, Ag     

An analytical investigation of elastic–plastic behaviors of 3D warp and woof auxetic structures

International Journal of Mechanics and Materials in Design - The present paper was conducted to calculate Young's modulus and Negative Poisson's ratio (NPR) of a warp and woof (WAW) 3D...     

On the microstructure of micro-pins manufactured by a novel progressive microforming process

Microforming is defined as the process of production of metallic micro-parts with at least two dimensions in sub-millimeter range. Many of these microforming processes have been investigated in laboratory-scale, which is not suitable for industrial applications. In this work, the feasibility of producing copper micro-pins using a novel progressive microforming process is demonstrated. This process has a good potential for mass production of micro-parts. The material flow behavior and the microstructure of the formed micro-pins were investigated by means of optical microscopy and simulation. From this study of material flow behavior with respect to different process conditions (die diameter, die design and punch diameters used), it will be shown how the respective material flow behavior in the progressive forming process influenced the microstructure evolution in the formed micro-pin. It was found in the experimental results that there is a soft zone on the micro-pins surface under specific process conditions. The microhardness results were consistent with the microstructural observations. Simulation was employed to understand the material flow direction under the punch during the microforming process and evaluate the position of the neutral zone in the disk-shape head of the micro-pin produced. This understanding of the neutral zone position with relation to the metal dead-zone as well as the material flow behavior was necessary to explain the dead-zone leakage in the microstructure and the occurrence of the soft zone. By decreasing the punch to die diameter ratio, and also choosing a die without entrance fillet radius, it was shown that the soft zones at the pin surface could be either minimized or entirely removed.     

Experimental investigation of adverse effect of frost formation on microchannel evaporators,part 1: Effect of fin geometry and environmental effects

This study experimentally investigated the frost growth on louvered folded fins in microchannel heat exchangers when used in outdoor air-source heat pump systems. The effects of surface temperature, fin geometries, and air environmental conditions were studied. The overall aim was to isolate and quantify the effect of geometry from surface temperature effects. Experimental data of frost weight, local frost thickness, air pressure drop across the coils, time of frost–defrost cycles and heat transfer rates were recorded. Data showed that the frosting time and the frost growth rates depended mainly on the local fin surface temperature. Lower fin density was beneficial because it delayed the blockage of the air flow. The fin length and fin depth had minor effects on frosting performance. The air humidity had a fairly significant effect on rate of frost formation while air velocity seemed to have a small effect on the frost growth rate.