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21.
SYBR®Green qPCR screening methods for the presence of “35S promoter” and “NOS terminator” elements in food and feed products 总被引:1,自引:1,他引:0
Elodie Barbau-Piednoir Antoon Lievens Guillaume Mbongolo-Mbella Nancy Roosens Myriam Sneyers Amaya Leunda-Casi Marc Van den Bulcke 《European Food Research and Technology》2010,230(3):383-393
The Cauliflower Mosaic Virus “35S promotor” (p35S) and the Agrobacterium “Nopaline Synthase” terminator (tNOS) are the most represented generic recombinant elements in commercial genetically modified crops to date. A set of four new SYBR®Green qPCR methods targeting the “p35S” and “tNOS” core elements have been developed. These qPCR methods generate short amplicons of 147 and 75 bp for the “p35S” element and 172 and 69 bp for the “tNOS” element. Single target plasmids containing these amplicons were constructed and allow determining the nominal melting temperature (T m value) of each amplicon. The four methods are specific for their respective targets, and moreover, three of them are highly sensitive (up to 1–2 copies detectable) at a PCR efficiency ranging between 95 and 100%. The latter methods can detect their respective targets at 0.1% (w/w) gDNA levels and are suitable for detecting low levels of genetically modified materials containing the “p35S” and/or “tNOS” elements. 相似文献
22.
<Emphasis Type="Italic">K</Emphasis>-space data processing for magnetic resonance elastography (MRE)
Nadège Corbin Elodie Breton Michel de Mathelin Jonathan Vappou 《Magma (New York, N.Y.)》2017,30(2):203-213
Objective
Magnetic resonance elastography (MRE) requires substantial data processing based on phase image reconstruction, wave enhancement, and inverse problem solving. The objective of this study is to propose a new, fast MRE method based on MR raw data processing, particularly adapted to applications requiring fast MRE measurement or high elastogram update rate.Materials and methods
The proposed method allows measuring tissue elasticity directly from raw data without prior phase image reconstruction and without phase unwrapping. Experimental feasibility is assessed both in a gelatin phantom and in the liver of a porcine model in vivo. Elastograms are reconstructed with the raw MRE method and compared to those obtained using conventional MRE. In a third experiment, changes in elasticity are monitored in real-time in a gelatin phantom during its solidification by using both conventional MRE and raw MRE.Results
The raw MRE method shows promising results by providing similar elasticity values to the ones obtained with conventional MRE methods while decreasing the number of processing steps and circumventing the delicate step of phase unwrapping. Limitations of the proposed method are the influence of the magnitude on the elastogram and the requirement for a minimum number of phase offsets.Conclusion
This study demonstrates the feasibility of directly reconstructing elastograms from raw data.23.
Hafnium is often used to improve the high temperature oxidation resistance of superalloys but not to form carbides for strengthen them against creep. In this work hafnium was added in cobalt-based alloys for verifying that HfC can be obtained in cobalt-based alloys and for characterizing their behavior at a very temperature. Three Co–25Cr–0.25 and 0.50C alloys containing 3.7 and 7.4 Hf to promote HfC carbides, and four Co–25Cr– 0 to 1C alloys for comparison (all contents in wt.%), were cast and exposed at 1200 °C for 50 h in synthetic air. The HfC carbides formed instead chromium carbides during solidification, in eutectic with matrix and as dispersed compact particles. During the stage at 1200 °C the HfC carbides did not significantly evolve, even near the oxidation front despite oxidation early become very fast and generalized. At the same time the chromium carbides present in the Co–Cr–C alloys totally disappeared in the same conditions. Such HfC-alloys potentially bring efficient and sustainable mechanical strengthening at high temperature, but their hot oxidation resistance must be significantly improved. 相似文献
24.
25.
Jean-Charles Sébileau Sébastien Lemonnier Elodie Barraud Marie-France Vallat Adele Carradò Michel Nardin 《应用聚合物科学杂志》2019,136(24):47645
This study deals with the understanding of the sintering mechanisms that occur during consolidation of an ultra-high-performance polymer: poly(ether-ether-ketone). In particular, we investigated the effects of uniaxial pressure during spark plasma sintering (SPS) processing. Glass-transition temperature (Tg) measurements under loading, stress–strain curves and scanning electron microscopy analysis allowed us to determine the role of pressure intensity and temperature of application on macromolecular chain mobility in both the bulk and the surface of the particles. First, a loss of chain mobility in the bulk of the particles was observed under high pressure. Second, it was shown that high pressure applied at low temperature leads to friction effects between the particles which enhances chain mobility in the particle shell through a local melting phenomenon. These experimental conditions favor the healing of particles and high cohesion was then observed in the resulting sintered samples. The level of friction is enhanced when pressure is applied at a low temperature since the powders are still in a glassy state. Finally, the achievement of cohesive grain boundaries was found to be related to the location and conformation of chain ends. Good healing between particles can only occur if a sufficient number of chain ends are available at the surface of the particle shell. We showed that the native powder state plays a significant role. The direct use of as-received powder leads to final material exhibiting good cohesion whereas pretreatments of the native powder are highly detrimental. It should be noted that this processing does not affect the high initial degree of crystallinity because no bulk melting is observed during consolidation by sintering. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136, 47645. 相似文献
26.
Dr. Youness Touati-Jallabe Dr. Thibault Tintillier Dr. Elodie Mauchauffée Dr. Jean-Luc Boucher Jérémy Leroy Booma Ramassamy Dr. Abdallah Hamzé Dr. Karima Mezghenna Amine Bouzekrini Claudia Verna Jean Martinez Dr. Anne-Dominique Lajoix Dr. Jean-François Hernandez 《ChemMedChem》2020,15(6):517-531
More than 160 arginine analogues modified on the C-terminus via either an amide bond or a heterocyclic moiety (1,2,4-oxadiazole, 1,3,4-oxadiazole and 1,2,4-triazole) were prepared as potential inhibitors of NO synthases (NOS). A methodology involving formation of a thiocitrulline intermediate linked through its side-chain on a solid support followed by modification of its carboxylate group was developed. Finally, the side-chain thiourea group was either let unchanged, S-alkylated (Me, Et) or guanidinylated (Me, Et) to yield respectively after TFA treatment the corresponding thiocitrulline, S-Me/Et-isothiocitrulline and N-Me/Et-arginine substrate analogues. They all were tested against three recombinant NOS isoforms. Several compounds containing a S-Et- or a S-Me-Itc moiety and mainly belonging to both the dipeptide-like and 1,2,4-oxadiazole series were shown to inhibit nNOS and iNOS with IC50 in the 1–50 μM range. Spectral studies confirmed that these new compounds interacted at the heme active site. The more active compounds were found to inhibit intra-cellular iNOS expressed in RAW264.7 and INS-1 cells with similar efficiency than the reference compounds L-NIL and SEIT. 相似文献
27.
Elodie Chiarovano Leigh A. McGarvie David Szmulewicz Hamish G. MacDougall 《Virtual Reality》2018,22(4):315-320
Subjective visual vertical (SVV) assesses the ability to perceive verticality, which is a measure of vestibular otolithic function. Vestibular lesions influence this perception of verticality. We developed a method using virtual reality (VR) display and an Android software application named ‘Curator SVV’. The virtual reality SVV (Curator SVV) consisted of ten readily identifiable artworks projected by a Samsung phone S6 which is inserted into a virtual reality headset. In the first study, 20 patients had there SVV assessed with two devices: (1) a commercially available SVV measurement device (VestiTest®) and (2) a virtual reality SVV using the Curator SVV application. In a second study, 32 healthy subjects had their SVV assessed by the Curator SVV application whilst sitting in a chair. In the first study, there was no significant difference (p = 0.44, paired t test and p = 0.01, test of equivalence) between results obtained by Curator SVV and the commercially available device. In the second study, the average angle measured for healthy subjects was 0.00° ± 0.85°. The normal range (mean ± 2 SD) was ± 2° in standard upright position. We were able to demonstrate that the Curator SVV can be readily employed as an objective, non-invasive and affordable means of assessing otolith function in the clinical context. We validated this novel methodology by finding strong quantitative parity between a standard commercial SVV unit and the VR Curator SVV method. Our very lightweight and mobile device can be employed in clinical contexts including at the bedside and in different head and body positions. 相似文献
28.
Nicolas Keller Elodie Barraud Florence Bosc David Edwards Valerie Keller 《Applied catalysis. B, Environmental》2007,70(1-4):423-430
The photocatalytic behavior of different TiO2-based photocatalysts was reported for gas-phase toluene removal under both UV and visible light illumination, and compared to that of commercial P25 (Degussa) TiO2. Promotion by sulfates and the use of nanosized anatase TiO2 were reported to strongly increase the toluene removal efficiency under UV illumination. Nanosized-anatase was prepared by a protecting group sol–gel synthesis using hexamethyldisilazane as crystallite growth inhibitor. Sulfates played a double positive role, with photogenerated electrons transfer effects limiting charge recombination and as repulsive species for strongly adsorbed aromatic intermediates that act as poisons. The decrease in particle size obtained on nanosized anatase TiO2 (5 nm) yielded a considerable enhancement in the toluene removal efficiency. Pure high surface area rutile has been synthesized at low temperature by a polyethylenglycol-containing sol–gel method for visible light activation purposes. A two-way semiconductor coupling phenomenon, consisting of a reciprocal electron/hole transfer between two visible light-activated oxides, rutile TiO2 and WO3, was proposed to explain the large gain in efficiency when adding low amounts of WO3 to rutile TiO2. 相似文献
29.
Jean‐Charles Sébileau Sébastien Lemonnier Elodie Barraud Marie‐France Vallat Adele Carradò Michel Nardin 《应用聚合物科学杂志》2017,134(23)
The present study deals with the consolidation of an ultra‐high performance polymer, the poly(ether ether ketone) (PEEK), for structural applications, using the powder metallurgy (PM) way, and more precisely the Spark Plasma Sintering (SPS) processing. The effects of SPS parameters such as temperature, pressure, and dwell time on density and mechanical properties of PEEK were investigated via a Design of Experiments (DoE). A temperature of 250 °C, a pressure of 40 MPa, and a dwell time of 20 min have been identified as the optimal SPS process parameters. In these conditions, a density of 1.31 g / cm3 was reached and homogeneous mechanical properties in the volume determined by means of compression tests were found with a compressive modulus of 2.75 GPa, a yield strength of 134 MPa, and a maximum compressive strain of 43%. These results are better than those of commercial products obtained by injection molding. The pressure appears to be a significant parameter on PEEK properties and plays positive or negative roles according to the responses of DoE studied. To our knowledge, it is one of the first studies based on the application of the PM techniques for PEEK consolidation showing the possibility to process below its melting point. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134, 44911. 相似文献
30.
Electrochemical oxidation of 2'-deoxyguanosine has been performed on boron-doped diamond (BDD) electrodes, resulting in a strong adsorption of the formed oxidized products onto the BDD surface. The adsorption behavior has been investigated by studying the electrochemical behavior of a redox probe ([IrCl6]3-) using cyclic voltammetry. The most probable situations are the formation of (A) an insulating adsorbed film resulting in a partially blocked electrode behavior, (B) a porous film, or (C) an overall conductive film. Different parameters such as the standard rate constant, the charge-transfer coefficient, the electrode/adsorbed products/solution interface resistance, and the formal potential of the redox couple were determined. Through comparison of theoretical current-potential curves obtained by analytical calculations with experimental cyclic voltammograms, we found that the oxidized products of 2'-deoxyguanosine form a continuous conductive film on BDD. 相似文献