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201.
Elena Stocco Silvia Barbon Elena Zeni Leonardo Cassari Annj Zamuner Antonio Gloria Teresa Russo Rafael Boscolo-Berto Maria Martina Sfriso Veronica Macchi Raffaele De Caro Monica Dettin Andrea Porzionato 《International journal of molecular sciences》2022,23(20)
Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs). Looking for OxPVA device optimization and coupling it with a natural sheath may boost bioactivity. Thus, OxPVA/chitosan sponges (ChS) as hybrid scaffolds were investigated to predict in the vivo behaviour of two-layered NCs. To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR. Thus, ChS and the hybrid scaffolds were characterized for mechanical properties, ultrastructure (Scanning Electron Microscopy, SEM), bioactivity, and biocompatibility. Regarding mechanical analysis, the peptide-free ChS showed the highest values of compressive modulus and maximum stress. However, among +EAK groups, ChS+EAK showed a significantly higher maximum stress than that found for ChS+EAK-IKVAV and ChS+EAK-YIGSR. Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells’ adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM. The scaffolds elicited only a slight inflammation after 14 days of subcutaneous implantation in Balb/c mice, proving biocompatibility. ChS porosity, EAK 3D features and neuro-friendly attitude (shared with IKVAV/YIGSR motifs) may confer to OxPVA certain bioactivity, laying the basis for future appealing NCs. 相似文献
202.
Jonathan O. Martinez Michael Evangelopoulos Ava A. Brozovich Guillermo Bauza Roberto Molinaro Claudia Corbo Xuewu Liu Francesca Taraballi Ennio Tasciotti 《Advanced functional materials》2021,31(3):2002997
The emergence of cell-based therapeutics, specifically the use of mesenchymal stromal/stem cells (MSCs), stands to significantly affect the future of targeted drug delivery technologies. MSCs represent a unique cell type, offering more than only regenerative potential but also site-specific inflammatory targeting and tissue infiltration. In this study, a versatile multicomponent delivery platform, combining MSC tropism with multistage nanovector (MSV)-mediated payload delivery, is debuted. It is demonstrated that the incorporation of drug-loaded MSVs bestows MSCs with the ability to transport anti-inflammatory payloads, achieving a fivefold increase in payload release without negatively impacting cellular functions, viability, extravasation, and inflammatory homing. When incorporated within MSCs, MSVs avoid rapid sequestration by filtering organs and conserve a 15-fold increase in local inflammatory targeting compared to healthy ears. Furthermore, this MSC-mediated MSV platform (M&Ms) rapidly triggers a 4.5-fold reduction of local inflammation compared to free drug and extends survival to 100% of treated mice in a lethal model of systemic inflammation. 相似文献
203.
CIB working commission W23, which deals with structural walls, has published design Recommendations for both reinforced and unreinforced masonry. The procedures set out for seismic design in reinforced masonry have been tested in a research project in Italy, and the results are presented here by Professors Calvi and Macchi from the University of Pavia. They show that buildings of 4 storeys in high-risk zones and of 5 storeys in moderate-risk zones can safely be designed in reinforced masonry according to the Recommendations, using highly perforated blocks only 300 mm thick. 相似文献
204.
The unfavorable effects of narrowband inputs on the decoder adjustment when the LMS algorithm is used are analyzed. An explanation is given for the behavior of the CCITT algorithm. Suboptimality of prediction is granted to achieve adjustment, resulting in a satisfactory tradeoff between reduction rate and adjustment. The link between adjustment and uniform stability is enhanced 相似文献
205.
In this work we prove the almost sure convergence of an adaptive linear estimator governed by a stochastic gradient algorithm with decreasing step size in the presence of correlated observations. Two complementary contributions are added to the famous 1977 Ljung theorem. First we drop the condition of nondivergence of the algorithm assumed by Ljung. While that condition can be ensured by adding a barrier, the convergence of the suitably bounded algorithm itself is not established even on the basis of Ljung theorem. Here, the barrier problem is overcome by proving that it is not necessary for the convergence. Our second contribution is to generalize the model describing the correlated observations. No state space model is used and no linear relationship between the observations and the signal to be estimated needs to be assumed. Instead we use a decreasing covariance model that agrees with a very wide class of practical applications. 相似文献
206.
Ennio Arimondo Massimo Inguscio 《Journal of Infrared, Millimeter and Terahertz Waves》1980,1(3):437-458
A careful analysis of the rotovibrational spectroscopic data of CH3CN is presented taking into account Fermi and Coriolis interactions. A large majority of the FIR lines obtained by optical pumping is assigned. New values are proposed for some molecular constants and the need of more accurate experimental investigation is pointed out. 相似文献
207.
The effect of water (H?O(g)) on in situ SO? capture using limestone injection under (FBC) conditions was studied using a thermobalance and tube furnace. The indirect sulphation reaction was found to be greatly enhanced in the presence of H?O(g). Stoichiometric conversion of samples occurred when sulphated with a synthetic flue gas containing 15% H?O(g) in under 10 h, which is equivalent to a 45% increase in conversion as compared to sulphation without H?O(g). Using gas pycnometry and nitrogen adsorption methods, it was shown that limestone samples sulphated in the presence of H?O(g) undergo increased particle densification without any significant changes to pore area or volume. The microstructural changes and observed increase in conversion were attributed to enhanced solid-state diffusion in CaO/CaSO? in the presence of H?O(g). Given steam has been shown to have such a strong influence on sulphation, whereas it had been previously regarded as inert, may prompt a revisiting of the classically accepted sulphation models and phenomena. These findings also suggest that steam injection may be used to enhance sulfur capture performance in fluidized beds firing low-moisture fuels such as petroleum coke. 相似文献
208.
Antonietta Baiano Carmela Terracone Giuseppe Gambacorta & Ennio La Notte 《International Journal of Food Science & Technology》2009,44(7):1304-1313
The purposes of this work were to determine isoflavones profile and antioxidant activity of two soy flours and spaghetti produced from semolina blended with increasing amount of these flours. Defatted and toasted soy flour (DSF and TSF, respectively) showed different isoflavone profiles though glucosides were the most abundant isoflavone forms in both the meals. The pasta-making procedure determined an increase of the aglycone forms that was at least 37 and 21% in TSF and DSF pasta, respectively. Cooking operations induced severe decreases of the isoflavone content (the losses ranged from 60.44 to104.91 mg kg−1 for TSF pasta and from 85.41 to 469.33 mg kg−1 for DSF pasta). The results of the antioxidant activity were quite different as a function of the methods applied but they resulted generally well correlated with the isoflavone contents of the samples. Cooking induced significant decreases of the antioxidant activity in almost all samples. This result appears well correlated with the decrease of the total isoflavone content and the presence of antioxidant activity in the cooking water. 相似文献
209.
I G de Kuck D D Krivoruchco O A Lucchini R A Macchi 《Archivos latinoamericanos de nutrición》1982,32(4):892-902
The composition and nutritional value of sunflower meals and protein isolates were determined. One of the meals was an ordinary industrial one (HI) and the other (HL) was prepared at the laboratory from the same batch of sunflower seeds. The protein isolates were obtained from both meals (AHI and AHL, respectively) and from the original seeds (AS), by means of a process employing water extraction and precipitation at pH 4.3. Chemical analysis of both meals showed that their moisture, protein crude fiber and fat contents had no significant difference. Sulphur amino acids (g/16 g N) were higher in the isolates than in the flours. The HL flour, and the AHL and AS isolates had a higher proportion of available lysine (96.9%, 93.0%, and 92.4% respectively) than the HI flour and AHI isolate. This improvement of protein quality was attained by the employment of less drastic thermal treatments in their preparation, as compared with the treatments to which other materials were submitted. 相似文献
210.
Barari Ahmad de Sales Guerra Tsuzuki Marcos Cohen Yuval Macchi Marco 《Journal of Intelligent Manufacturing》2021,32(7):1793-1796
Journal of Intelligent Manufacturing - 相似文献