Early Osteogenic Marker Expression in hMSCs Cultured onto Acid Etching-Derived Micro- and Nanotopography 3D-Printed Titanium Surfaces

Polyetheretherketone (PEEK) titanium composite (PTC) is a novel interbody fusion device that combines a PEEK core with titanium alloy (Ti6Al4V) endplates. The present study aimed to investigate the in vitro biological reactivity of human bone-marrow-derived mesenchymal stem cells (hBM-MSCs) to micro- and nanotopographies produced by an acid-etching process on the surface of 3D-printed PTC endplates. Optical profilometer and scanning electron microscopy were used to assess the surface roughness and identify the nano-features of etched or unetched PTC endplates, respectively. The viability, morphology and the expression of specific osteogenic markers were examined after 7 days of culture in the seeded cells. Haralick texture analysis was carried out on the unseeded endplates to correlate surface texture features to the biological data. The acid-etching process modified the surface roughness of the 3D-printed PTC endplates, creating micro- and nano-scale structures that significantly contributed to sustaining the viability of hBM-MSCs and triggering the expression of early osteogenic markers, such as alkaline phosphatase activity and bone-ECM protein production. Finally, the topography of 3D-printed PTC endplates influenced Haralick’s features, which in turn correlated with the expression of two osteogenic markers, osteopontin and osteocalcin. Overall, these data demonstrate that the acid-etching process of PTC endplates created a favourable environment for osteogenic differentiation of hBM-MSCs and may potentially have clinical benefit.     

Preclinical Assessment of the Combination of PSMA-Targeting Radionuclide Therapy with PARP Inhibitors for Prostate Cancer Treatment

Prostate specific membrane antigen targeted radionuclide therapy (PSMA-TRT) is a promising novel treatment for prostate cancer (PCa) patients. However, PSMA-TRT cannot be used for curative intent yet, thus additional research on how to improve the therapeutic efficacy is warranted. A potential way of achieving this, is combining TRT with poly ADP-ribosylation inhibitors (PARPi), which has shown promising results for TRT of neuroendocrine tumor cells. Currently, several clinical trials have been initiated for this combination for PCa, however so far, no evidence of synergism is available for PCa. Therefore, we evaluated the combination of PSMA-TRT with three classes of PARPi in preclinical PCa models. In vitro viability and survival assays were performed using PSMA-expressing PCa cell lines PC3-PIP and LNCaP to assess the effect of increasing concentrations of PARPi veliparib, olaparib or talazoparib in combination with PSMA-TRT compared to single PARPi treatment. Next, DNA damage analyses were performed by quantifying the number of DNA breaks by immunofluorescent stainings. Lastly, the potential of the combination treatments was studied in vivo in mice bearing PC3-PIP xenografts. Our results show that combining PSMA-TRT with PARPi did not synergistically affect the in vitro clonogenic survival or cell viability. DNA-damage analysis revealed only a significant increase in DNA breaks when combining PSMA-TRT with veliparib and not in the other combination treatments. Moreover, PSMA-TRT with PARPi treatment did not improve tumor control compared to PSMA-TRT monotherapy. Overall, the data presented do not support the assumption that combining PSMA-TRT with PARPi leads to a synergistic antitumor effect in PCa. These results underline that extensive preclinical research using various PCa models is imperative to validate the applicability of the combination strategy for PCa, as it is for other cancer types.     

Localized Heat Therapy Improves Mitochondrial Respiratory Capacity but Not Fatty Acid Oxidation

AIM: Mild heat stress can improve mitochondrial respiratory capacity in skeletal muscle. However, long-term heat interventions are scarce, and the effects of heat therapy need to be understood in the context of the adaptations which follow the more complex combination of stimuli from exercise training. The purpose of this work was to compare the effects of 6 weeks of localized heat therapy on human skeletal muscle mitochondria to single-leg interval training. METHODS: Thirty-five subjects were assigned to receive sham therapy, short-wave diathermy heat therapy, or single-leg interval exercise training, localized to the quadriceps muscles of the right leg. All interventions took place 3 times per week. Muscle biopsies were performed at baseline, and after 3 and 6 weeks of intervention. Mitochondrial respiratory capacity was assessed on permeabilized muscle fibers via high-resolution respirometry. RESULTS: The primary finding of this work was that heat therapy and exercise training significantly improved mitochondrial respiratory capacity by 24.8 ± 6.2% and 27.9 ± 8.7%, respectively (p < 0.05). Fatty acid oxidation and citrate synthase activity were also increased following exercise training by 29.5 ± 6.8% and 19.0 ± 7.4%, respectively (p < 0.05). However, contrary to our hypothesis, heat therapy did not increase fatty acid oxidation or citrate synthase activity. CONCLUSION: Six weeks of muscle-localized heat therapy significantly improves mitochondrial respiratory capacity, comparable to exercise training. However, unlike exercise, heat does not improve fatty acid oxidation capacity.     

Face Detection: A Survey

In this paper we present a comprehensive and critical survey of face detection algorithms. Face detection is a necessary first-step in face recognition systems, with the purpose of localizing and extracting the face region from the background. It also has several applications in areas such as content-based image retrieval, video coding, video conferencing, crowd surveillance, and intelligent human–computer interfaces. However, it was not until recently that the face detection problem received considerable attention among researchers. The human face is a dynamic object and has a high degree of variability in its apperance, which makes face detection a difficult problem in computer vision. A wide variety of techniques have been proposed, ranging from simple edge-based algorithms to composite high-level approaches utilizing advanced pattern recognition methods. The algorithms presented in this paper are classified as either feature-based or image-based and are discussed in terms of their technical approach and performance. Due to the lack of standardized tests, we do not provide a comprehensive comparative evaluation, but in cases where results are reported on common datasets, comparisons are presented. We also give a presentation of some proposed applications and possible application areas.     

Alternative Tilings for Improved Surface Area Estimates by Local Counting Algorithms

In this paper, we first review local counting methods for perimeter estimation of piecewise smooth binary figures on square, hexagonal, and triangular grids. We verify that better perimeter estimates, using local counting algorithms, can be obtained using hexagonal or triangular grids. We then compare surface area estimates using local counting techniques for binary three-dimensional volumes under the three semi-regular polyhedral tilings: the cubic, truncated octahedral, and rhombic dodecahedral tilings. It is shown that for surfaces of random orientation with a uniform distribution, the expected error of surface area estimates is smaller for the truncated octahedral and rhombic dodecahedral tilings than for the standard cubic or rectangular prism tilings of space. Additional properties of these tessellations are reviewed and potential applications of better surface area estimates are discussed.     

爱立信IMS weShare分享每一天(系列之二)如何提供出色的用户体验?

爱立信IMS weShare业务有20%的开发与用户视觉体验相关(用户界面、图标、标签以及进度条),其它的80%则主要致力于设备间的系统.与此相对比,用户的体验则完全(100%)有赖于其可接触的事物.     

Über die Säuren HOOCCH(SX)CH(SX)COOH (X  CH2COOH,C6H5) und ihre Methylester

The Acids HOOCCH(SX)CH(SX)COOH (X  CH₂COOH, C₆H₅) and their Methyl Esters The acids HOOCCH(SX)CH(SX)COOH with X  CH₂COOH and C₆H₅ and their methyl esters have been prepared by addition of thiols to acetylene dicarboxylic acid as salt and methyl ester. The structures and configurations have been discussed.     

Concept-Driven Interaction Design Research

In this article, we explore a concept-driven approach to interaction design research with a specific focus on theoretical advancements. We introduce this approach as a complementary approach to more traditional, and well-known, user-centered interaction design approaches. A concept-driven approach aims at manifesting theoretical concepts in concrete designs. A good concept design is both conceptually and historically grounded, bearing signs of the intended theoretical considerations. In the area of human–computer interaction and interaction design research, this approach has been quite popular but not necessarily explicitly recognized and developed as a proper research methodology. In this article, we demonstrate how a concept-driven approach can coexist, and be integrated with, common user-centered approaches to interaction design through the development of a model that makes explicit the existing cycle of prototyping, theory development, and user studies. We also present a set of basic principles that could constitute a foundation for concept driven interaction research, and we have considered and described the methodological implications given these principles. For the field of interaction design research we find this as an important point of departure for taking the next step toward the construction and verification of theoretical constructs that can help inform and guide future design research projects on novel interaction technologies.     

A methodology for user directed search in evolutionary design

A designer should be able to express their intentions with a design tool. This work describes a methodology that enables the architect to directly interact with the encoding of designs they find aesthetically pleasing. Broadening interaction beyond simple evaluation increases the amount of feedback and bias a user can apply to the search. Increased feedback will have the effect of directing the algorithm to more fruitful areas of the search space. We conduct trials on two interfaces for making localised changes to a design in order to evaluate if the user is capable of directing search. In addition, an examination of the locality of changes made by the users provides an insight into how they explore the search space. The results show that a suitably designed interface is capable of directing search and that the participants used different magnitudes of change during directed search.