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991.
Lorenzo Barba Steffen Halbgebauer Federico Paolini Paoletti Giovanni Bellomo Samir Abu-Rumeileh Petra Steinacker Federico Massa Lucilla Parnetti Markus Otto 《International journal of molecular sciences》2022,23(13)
SerpinA1 (α1-antitrypsin) is a soluble glycoprotein, the cerebrospinal fluid (CSF) isoforms of which showed disease-specific changes in neurodegenerative disorders that are still unexplored in Alz-heimer’s disease (AD). By means of capillary isoelectric focusing immunoassay, we investigated six serpinA1 isoforms in CSF samples of controls (n = 29), AD-MCI (n = 29), AD-dem (n = 26) and Lewy body disease (LBD, n = 59) patients and correlated the findings with CSF AD core biomarkers (Aβ42/40 ratio, p-tau, t-tau). Four CSF serpinA1 isoforms were differently expressed in AD patients compared to controls and LBD patients, especially isoforms 2 and 4. AD-specific changes were found since the MCI stage and significantly correlated with decreased Aβ42/40 (p < 0.05) and in-creased p-tau and t-tau levels in CSF (p < 0.001). Analysis of serpinA1 isoform provided good di-agnostic accuracy in discriminating AD patients versus controls (AUC = 0.80) and versus LBD patients (AUC = 0.92), with best results in patients in the dementia stage (AUC = 0.97). SerpinA1 isoform expression is altered in AD patients, suggesting a common, albeit disease-specific, in-volvement of serpinA1 in most neurodegenerative disorders. 相似文献
992.
Annunziata Nusca Francesco Piccirillo Federico Bernardini Aurelio De Filippis Federica Coletti Fabio Mangiacapra Elisabetta Ricottini Rosetta Melfi Paolo Gallo Valeria Cammalleri Nicola Napoli Gian Paolo Ussia Francesco Grigioni 《International journal of molecular sciences》2022,23(13)
Coronary artery disease (CAD) remains one of the most important causes of morbidity and mortality worldwide, and revascularization through percutaneous coronary interventions (PCI) significantly improves survival. In this setting, poor glycaemic control, regardless of diabetes, has been associated with increased incidence of peri-procedural and long-term complications and worse prognosis. Novel antidiabetic agents have represented a paradigm shift in managing patients with diabetes and cardiovascular diseases. However, limited data are reported so far in patients undergoing coronary stenting. This review intends to provide an overview of the biological mechanisms underlying hyperglycaemia-induced vascular damage and the contrasting actions of new antidiabetic drugs. We summarize existing evidence on the effects of these drugs in the setting of PCI, addressing pre-clinical and clinical studies and drug-drug interactions with antiplatelet agents, thus highlighting new opportunities for optimal long-term management of these patients. 相似文献
993.
Dr. Elena Casanova Dr. David Moreno Alba Gigante Dr. Eva Rico Carlos Mario Genes Cristina Oliva Prof. María‐José Camarasa Prof. Federico Gago Prof. Antonio Jiménez‐Ruiz Prof. María‐Jesús Pérez‐Pérez 《ChemMedChem》2013,8(7):1161-1174
Two series of 5′‐triphenylmethyl (trityl)‐substituted thymidine derivatives were synthesized and tested against Leishmania infantum axenic promastigotes and amastigotes. Several of these compounds show significant antileishmanial activity, with IC50 values in the low micromolar range. Among these, 3′‐O‐(isoleucylisoleucyl)‐5′‐O‐(3,3,3‐triphenylpropanoyl)thymidine displays particularly good activity against intracellular amastigotes. Assays performed to characterize the nature of parasite cell death in the presence of the tritylthymidines indicated significant alterations in mitochondrial transmembrane potential, an increase in superoxide concentrations, and also significant decreases in DNA degradation during the cell death process. Results point to the mitochondrial nuclease LiEndoG as a target for the action of this family of compounds. 相似文献
994.
Federico Smeacetto Auristela De Miranda Andreas Chrysanthou Enrico Bernardo Michele Secco Massimiliano Bindi Milena Salvo Antonio G. Sabato Monica Ferraris 《Journal of the American Ceramic Society》2014,97(12):3835-3842
This work deals with the design, the characterization, and testing of a novel glass‐ceramic to be used as sealant for planar solid oxide fuel cells and its compatibility with Mn1.5Co1.5O4‐coated Crofer22APU. Thermal, sintering, and crystallization behavior and thermo mechanical properties of the sealant are reviewed and discussed, indicating therefore that these compositions can be deposited at 850°C and provide an excellent compatibility with both the Mn1.5Co1.5O4‐coated Crofer22APU and the anode‐supported electrolyte. In particular, Mn1.5Co1.5O4‐coated Crofer22APU/sealant/anode‐supported‐electrolyte joined samples have been submitted to thermal tests (in air atmosphere) from RT to 800°C (SOFC operating temperature) up to 500 h. No interactions, cracks formation, or failure were observed at the Mn1.5Co1.5O4‐coated Crofer22APU/sealant interface and between the glass‐ceramic and the anode‐supported‐electrolyte after 500 h of thermal tests in air atmosphere. 相似文献
995.
Martina Bollati Chiara Lopez Fabio Bioletto Federico Ponzetto Ezio Ghigo Mauro Maccario Mirko Parasiliti-Caprino 《International journal of molecular sciences》2022,23(4)
Primary aldosteronism (PA) is the most common cause of secondary hypertension. A growing body of evidence has suggested that, beyond its well-known effects on blood pressure and electrolyte balance, aldosterone excess can exert pro-inflammatory, pro-oxidant and pro-fibrotic effects on the kidney, blood vessels and heart, leading to potentially harmful pathophysiological consequences. In clinical studies, PA has been associated with an increased risk of cardiovascular, cerebrovascular, renal and metabolic complication compared to essential hypertension, including atrial fibrillation (AF) and aortic ectasia. An increased prevalence of AF in patients with PA has been demonstrated in several clinical studies. Aldosterone excess seems to be involved in the pathogenesis of AF by inducing cardiac structural and electrical remodeling that in turn predisposes to arrhythmogenicity. The association between PA and aortic ectasia is less established, but several studies have demonstrated an effect of aldosterone on aortic stiffness, vascular smooth muscle cells and media composition that, in turn, might lead to an increased risk of aortic dilation and dissection. In this review, we focus on the current evidence regarding the potential role of aldosterone excess in the pathogenesis of AF and aortic ectasia. 相似文献
996.
In this paper we show a new method for calculating the nucleolus by solving a unique minimization linear program with O(4n) constraints whose coefficients belong to {−1,0,1}. We discuss the need of having all these constraints and empirically prove that they can be reduced to O(kmax2n), where kmax is a positive integer comparable with the number of players. A computational experience shows the applicability of our method over (pseudo)random transferable utility cooperative games with up to 18 players. 相似文献
997.
Andrea Santos Ion Cristbal Jaime Rubio Cristina Carams Melani Luque Marta Sanz-lvarez Sandra Zazo Juan Madoz-Gúrpide Federico Rojo Jesus García-Foncillas 《International journal of molecular sciences》2022,23(20)
The standard clinical management of locally advanced rectal cancer (LARC) patients includes neoadjuvant 5-fluorouracil (5-FU)-based chemoradiotherapy (CRT) followed by mesorectal excision. MicroRNA (miR)-19b expression levels in LARC biopsies obtained from initial colonoscopy have recently been identified as independent predictors of both patient outcome and pathological response to preoperative CRT in this disease. Moreover, it has been discovered that this miR increases its expression in 5-FU resistant colon cancer cells after 5-FU exposure. Despite the fact that these observations suggest a functional role of miR-19b modulating 5-FU response of LARC cells, this issue still remains to be clarified. Here, we show that downregulation of miR-19b enhances the antitumor effects of 5-FU treatment. Moreover, ectopic miR-19b modulation was able to restore sensitivity to 5-FU treatment using an acquired resistant model to this compound. Notably, we also evaluated the potential clinical impact of miR-19b as a predictive marker of disease progression after tumor surgery resection in LARC patients, observing that miR-19b overexpression significantly anticipates patient recurrence in our cohort (p = 0.002). Altogether, our findings demonstrate the functional role of miR-19b in the progressively decreasing sensitivity to 5-FU treatment and its potential usefulness as a therapeutic target to overcome 5-FU resistance, as well as its clinical impact as predictor of tumor progression and relapse. 相似文献
998.
Valentino Le Noci Giancarla Bernardo Giacomo Manenti Gabriele Infante Dariush Khaleghi Hashemian Lucia Minoli Simone Canesi Francesca Bianchi Tiziana Triulzi Stefania Arioli Loris De Cecco Simone Guglielmetti Federico Ambrogi Camilla Recordati Nicoletta Gagliano Elda Tagliabue Michele Sommariva Lucia Sfondrini 《International journal of molecular sciences》2022,23(21)
999.
1000.
Laura Caliogna Valentina Bina Alice Maria Brancato Giulia Gastaldi Salvatore Annunziata Mario Mosconi Federico Alberto Grassi Francesco Benazzo Gianluigi Pasta 《International journal of molecular sciences》2022,23(22)
Bone responses to pulsed electromagnetic fields (PEMFs) have been extensively studied by using devices that expose bone cells to PEMFs to stimulate extracellular matrix (ECM) synthesis for bone and cartilage repair. The aim of this work was to highlight in which bone healing phase PEMFs exert their action. Specifically, we evaluated the effects of PEMFs both on human adipose mesenchymal stem cells (hASCs) and on primary human osteoblasts (hOBs) by testing gene and protein expression of early bone markers (on hASCs) and the synthesis of late bone-specific proteins (on hOBs) as markers of bone remodeling. Our results indicate that PEMFs seem to exert their action on bone formation, acting on osteogenic precursors (hASCs) and inducing the commitment towards the differentiation pathways, unlike mature and terminally differentiated cells (hOBs), which are known to resist homeostasis perturbation more and seem to be much less responsive than mesenchymal stem cells. Understanding the role of PEMFs on bone regenerative processes provides important details for their clinical application. 相似文献