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Ting Sun Xian Li Xiaochuan Jin Ziyi Wu Xiachao Chen Jieqiong Qiu 《International journal of molecular sciences》2022,23(11)
Graphene oxide is well known for its excellent fluorescence quenching ability. In this study, positively charged graphene oxide (pGO25000) was developed as a fluorescence quencher that is water-soluble and synthesized by grafting polyetherimide onto graphene oxide nanosheets by a carbodiimide reaction. Compared to graphene oxide, the fluorescence quenching ability of pGO25000 is significantly improved by the increase in the affinity between pGO25000 and the DNA strand, which is introduced by the additional electrostatic interaction. The FAM-labeled single-stranded DNA probe can be almost completely quenched at concentrations of pGO25000 as low as 0.1 μg/mL. A simple and novel FAM-labeled single-stranded DNA sensor was designed for Hg2+ detection to take advantage of exonuclease I-triggered single-stranded DNA hydrolysis, and pGO25000 acted as a fluorescence quencher. The FAM-labeled single-stranded DNA probe is present as a hairpin structure by the formation of T–Hg2+–T when Hg2+ is present, and no fluorescence is observed. It is digested by exonuclease I without Hg2+, and fluorescence is recovered. The fluorescence intensity of the proposed biosensor was positively correlated with the Hg2+ concentration in the range of 0–250 nM (R2 = 0.9955), with a seasonable limit of detection (3σ) cal. 3.93 nM. It was successfully applied to real samples of pond water for Hg2+ detection, obtaining a recovery rate from 99.6% to 101.1%. 相似文献
54.
Xiao Zhang Daoming Liu Dong Gao Weining Zhao Huaying Du Zeyu Qiu Jie Huang Peizheng Wen Yongsheng Wang Qi Li Wenhui Wang Haosen Xu Jun He Yuqiang Liu Jianmin Wan 《International journal of molecular sciences》2022,23(11)
Plants have evolved a sophisticated defense system that employs various hormone pathways to defend against attacks by insect pests. Cytokinin (CK) plays an important role in plant growth and stress tolerance, but the role of CKs in plant–insect interaction remains largely unclear. Here, we report that CKs act as a positive regulator in rice resistance against brown planthopper (BPH), a devastating insect pest of rice. We found that BPH feeding promotes CK biosynthesis and signaling in rice. Exogenous application of CKs significantly increased the rice resistance to BPH. Increasing endogenous CKs by knocking out cytokinin oxidase/dehydrogenase (OsCKXs) led to enhanced resistance to BPH. Moreover, the levels of the plant hormone jasmonic acid (JA) and the expression of JA-responsive genes were elevated by CK treatment and in OsCKXs knockout plants. Furthermore, JA-deficient mutant og1 was more susceptible to BPH, and CK-induced BPH resistance was suppressed in og1. These results indicate that CK-mediated BPH resistance is JA-dependent. Our findings provide the direct evidence for the novel role of CK in promoting insect resistance, and demonstrate that CK-induced insect resistance is JA-dependent. These results provide important guidance for effective pest management strategies in the future. 相似文献
55.
Chuanyang Liu Lu Min Jingyu Kuang Chushu Zhu Xinyuan Qiu Xiaomin Wu Tianyi Zhang Sisi Xie Lingyun Zhu 《International journal of molecular sciences》2022,23(12)
The pivotal roles of miRNAs in carcinogenesis, metastasis, and prognosis have been demonstrated recently in various cancers. This study intended to investigate the specific roles of hsa-miR-654-5p in lung cancer, which is, in general, rarely discussed. A series of closed-loop bioinformatic functional analyses were integrated with in vitro experimental validation to explore the overall biological functions and pan-cancer regulation pattern of miR-654-5p. We found that miR-654-5p abundance was significantly elevated in LUAD tissues and correlated with patients’ survival. A total of 275 potential targets of miR-654-5p were then identified and the miR-654-5p-RNF8 regulation axis was validated in vitro as a proof of concept. Furthermore, we revealed the tumor-suppressing roles of miR-654-5p and demonstrated that miR-654-5p inhibited the lung cancer cell epithelial-mesenchymal transition (EMT) process, cell proliferation, and migration using target-based, abundance-based, and ssGSEA-based bioinformatic methods and in vitro validation. Following the construction of a protein–protein interaction network, 11 highly interconnected hub genes were identified and a five-genes risk scoring model was developed to assess their potential prognostic ability. Our study does not only provide a basic miRNA-mRNA-phenotypes reference map for understanding the function of miR-654-5p in different cancers but also reveals the tumor-suppressing roles and prognostic values of miR-654-5p. 相似文献
56.
Jingjin Dong Jian Liu Xinkai Qiu Ryan Chiechi L.Jan Anton Koster Giuseppe Portale 《工程(英文)》2021,7(5):647-654
Engineering the electrical properties of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate)(PEDOT:PSS)holds great potential for various applications such a... 相似文献
57.
针对整县光伏背景下规模化分布式光伏接入配电网导致的电压波动问题,提出了一种基于近邻传播聚类(affinity propagation,AP)与鲸鱼算法(whale optimization algorithm,WOA)优化门控循环单元(gated recurrent unit,GRU)的分布式光伏集群电压越限预测方法。首先,在考虑分布式光伏地理坐标气象特征的基础上,添加基于配电网节点负荷密度因素的位置特征,采用近邻传播聚类方法,在不指定聚类数目的情况下划分具有近似气象特征和地理位置特征的分布式光伏集群,提高模型训练效果及适应性;然后,采用鲸鱼优化算法全局搜索GRU模型的最优训练参数,进一步提高模型的训练速度和预测精度;最后,利用WOA-GRU组合模型实现配电网节点电压与环境温度、光照强度的关联匹配,进而实现区域配电网电压波动及电压越限情况的整体预测。实验证明:所提出的方法能够有效提高预测精度及训练速度,强化预测模型的适应能力,具有较好的经济性和实用性。 相似文献
58.
Zicheng Li Yifeng Gao Zhihao Zhang Qiu Xiong Longhui Deng Xiaochun Li Qin Zhou Yuanxing Fang Peng Gao 《纳微快报(英文)》2021,(7):12-27
Efficient electron transport layers(ETLs)not only play a crucial role in promoting carrier separation and electron extraction in perovskite solar cells(PSCs)but... 相似文献
59.
The vacuum thermal evaporation technique was used to simultaneously deposit zinc coatings onto interstitial free steel plates and single-crystal silicon wafers ... 相似文献
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