Hypolimnetic redox and phosphorus cycling in hypereutrophic Lake Sebasticook, Maine

The vertical distribution of reduced species and phosphate was monitored throughout a summer stagnation period in hypereutrophic Lake Sebasticook, Maine. The oxygen demand of reduced species released from hypolimnetic sediments was dominated by methane, followed by ammonium, and included minor contributions from Fe²⁺, Mn²⁺ and sulfide. Release of iron and manganese from the sediments was apparently controlled by dissolution of siderite and rhodochrosite, with the requisite acidity for the dissolution provided by methanogenesis. Redox recycling of these two metals occurs in two vertically displaced “wheels”, which enhance oxidant delivery to the hypolimnion beyond that provided by oxygen diffusion alone. Phosphorus is tightly coupled to the “ferrous wheel”, in the absence of wind events, and is scavenged with a stoichiometry consistent with Tessenow's (Tessenow U., Arch. Hydrobiol.Suppl. 47, 1–79, 1974) laboratory studies.     

Marquis-K cultivar standard for wheat gliadin electrophoresis

Gliadins from single kernels and bulk samples (1 g) of the common wheat cultivar Marquis grown in different years and from different seed sources were separated by polyacrylamide gel electrophoresis. Gliadin electrophoregrams from inbred stocks of Marquis derived from a single-plant selection propagated over a period of 13 years were identical. Single seed analysis indicated differences in gliadin composition of Marquis obtained from different sources. It is recommended that the inbred stock referred to as Marquis-K be adopted as the standard for this cultivar.     

Non-Parametric Sensitivity Analysis in Linear Programming

A linear programming problem subject to uncertainty in the requirements vector is solved deterministically, then sensitivity analysis is performed to determine the effect of the random variation on this solution. Since there could be an appreciable cost of modifying a solution once implemented, the probability of the random components perturbing a solution is considered. Unlike existing methods of linear programming under uncertainty, this article assumes no knowledge of the distributions of the random variables. Rather, the notion of non-parametric tolerance limits is employed to establish a criterion for changing basic solutions.     

Quantum size effect in thin films

When the thickness of a film is of the order of the de Broglie wavelength of the electron, size effects appear in the electrical properties of the material. In this paper we derive the current in the presence of a magnetic field perpendicular to the plane of the film. We use the quantum mechanical density-matrix formalism. Unlike previous works we obtain an exact expression for the current. It is found that the current has a non-linear behavior with the electric field. The current also oscillates with the thickness of the film.     

Prevalence of high-risk egg-preparation practices in restaurants that prepare breakfast egg entrées: an EHS-Net study

Salmonella enterica serotype Enteritidis (SE) is a common cause of foodborne illness in the United States. Foods prepared with raw shell eggs have often been associated with SE outbreaks. The federal government published the Egg Safety Action Plan in December 1999 that called for reduction of egg-preparation practices that may contribute to the survival and proliferation of SE. In seven states, an interview and brief site evaluation of 153 restaurants that prepare eggs during all hours of operation was conducted by the Environmental Health Specialists Network to determine the prevalence of such practices. Fifty-four percent (83 of 153) of restaurants pooled raw shell eggs not intended for immediate service. These pooled eggs were held a median of 4 h for scrambled eggs, 5.5 h for omelets, and 6 h for pancakes and French toast. Nearly 26% (39 of 152) of restaurants reported storing eggs at room temperature, and 5% (7 of 152) stored eggs on ice or in cold-water baths before cooking. Generally, eggs were cooked to 72 to 83 degrees C, which is above the recommended final cook temperature of 63 to 68 degrees C. Employees reported sanitizing utensils used to prepare eggs less than once every 4 h in 42% (57 of 136) of restaurants. Several areas were identified in which further emphasis might reduce egg-associated SE infections in accordance with Healthy People 2010 goals.     

DNA mutation detection in a polymer microfluidic network using temperature gradient gel electrophoresis

A miniaturized system for DNA mutation analysis, utilizing temperature gradient gel electrophoresis (TGGE) in a polycarbonate (PC) microfluidic device, is reported. TGGE reveals the presence of sequence heterogeneity in a given heteroduplex sample by introducing a thermal denaturing gradient that results in differences between the average electrophoretic mobilities of DNA sequence variants. Bulk heater assemblies are designed and employed to externally generate temperature gradients in spatial and temporal formats along the separation channels. TGGE analyses of model mutant DNA fragments, each containing a single base substitution, are achieved using both single- and 10-channel parallel measurements in a microfluidic platform. Additionally, a comprehensive polymer microfluidic device containing an integrated microheater and sensor array is developed and demonstrated for performing spatial TGGE for DNA mutation analysis. The device consists of two PC modular substrates mechanically bonded together. One substrate is embossed with microchannels, and the other contains a tapered microheater, lithographically patterned along with an array of temperature sensors. Compared with the external heating approaches, the integrated platform provides significant reduction in power requirement and thermal response time while establishing more accurate and highly effective control of the temperature gradient for achieving improved separation resolution.     

Comparison of SeaWiFS measurements of the Moon with the U.S. Geological Survey lunar model

The Sea-Viewing Wide-Field-of-View Sensor (SeaWiFS) has made monthly observations of the Moon since 1997. Using 66 monthly measurements, the SeaWiFS calibration team has developed a correction for the instrument's on-orbit response changes. Concurrently, a lunar irradiance model has been developed by the U.S. Geological Survey (USGS) from extensive Earth-based observations of the Moon. The lunar irradiances measured by SeaWiFS are compared with the USGS model. The comparison shows essentially identical response histories for SeaWiFS, with differences from the model of less than 0.05% per thousand days in the long-term trends. From the SeaWiFS experience we have learned that it is important to view the entire lunar image at a constant phase angle from measurement to measurement and to understand, as best as possible, the size of each lunar image. However, a constant phase angle is not required for using the USGS model. With a long-term satellite lunar data set it is possible to determine instrument changes at a quality level approximating that from the USGS lunar model. However, early in a mission, when the dependence on factors such as phase and libration cannot be adequately determined from satellite measurements alone, the USGS model is critical to an understanding of trends in instruments that use the Moon for calibration. This is the case for SeaWiFS.     

Restricted use of success cues in retrieval during posthypnotic amnesia.

Evaluated memory for successful and unsuccessful responses to hypnotic suggestions in partially amnesic Ss and in those Ss with normal forgetting. Two analyses (278 undergraduates) demonstrated that highly hypnotizable Ss experiencing partial posthypnotic amnesia tended to show no selective recall for their successes or failures during amnesia, whereas the remainder of the Ss showed definite selective recall of hypnotic success posthypnotically. These findings support F. J. Evans and J. F. Kihlstrom's (see record 1974-06307-001) hypothesis that posthypnotic amnesia involves a disruption of memory organization and suggest that the phenomenon may be mediated by a restriction in the use of normally employed retrieval cues. (37 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Treatment of the drinking driver.

Examined the effect on the 2-yr driving record of drinking drivers referred by the courts to 6 programs for individual treatment. 2,723 drivers treated were classified as light or heavy drinkers, and compared with a no-treatment group similarly classified (n?=?2,127). Outcome criteria were (1) alcohol-related violations, (2) total moving violations, (3) alcohol-related accidents, and (4) total number of accidents. For light drinkers, 5 of the 6 countermeasures were significantly successful on at least 2 of the outcome measures, whereas 1 of them produced a significant decrease on all 4 criteria. Heavy drinkers apparently did not respond to treatment or had significantly worse records than those not treated. It is concluded that a variety of programs successfully treat the light drinker, but many existing programs result in worse records for the heavy drinker. (17 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Calibration and evaluation of nitric acid and ammonia permeation tubes by UV optical absorption

An ultraviolet (UV) optical absorption system has been developed for absolute calibrations of nitric acid (HNO3) and ammonia (NH3) permeation tube emission rates. Using this technique, dilute mixtures containing NH3 or HNO3, both of which interact strongly with many surfaces, are accurately measured at levels below a part per million by volume. This compact and portable instrument operates continuously and autonomously to rapidly (<1 h) quantify the emission of trace gases from permeation devices that are commonly used to calibrate air-monitoring instruments. The output from several HNO3 and NH3 permeation tubes, with emission rates that ranged between 13 and 150 ng/min, was examined as a function of temperature, pressure, and carrier gas flow. Absorptions of 0.015% can be detected which allows a precision (3sigma) of +/-1 ng/min for the HNO3 and NH3 permeation tubes studied here. The accuracy of the measurements, which relies on published UV absorption cross sections, is estimated to be +/-10%. Measurements of permeation tube emission rates using ion chromatography analysis are made to further assess measurement accuracy. The output from the HNO3 and NH3 permeation tubes examined here was stable over the study period, which ranged between 3 months and 1 year for each permeation tube.