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41.
Anna Giovanna Sciancalepore Elisa Mele Valentina Arcadio Francesco Reddavide Francesco Grieco Giuseppe Spano Patrick Lucas Giovanni Mita Dario Pisignano 《Food microbiology》2013
The development of fast, reliable and culture-independent molecular tools to detect bacteria producing biogenic amines deserves the attention of research and ultimately of the food industry in order to protect consumers' health. Here we present the application of a simple, low-cost, fast and sensitive method to perform microdroplet-based multiplex PCR, directly on a food matrix, for the simultaneous detection of bacterial genes involved in biogenic amine biosynthesis. After inoculating wine with Lactobacillus brevis IOEB 9809, cell lysis and DNA amplification are performed in one single step, without preliminary nucleic acid extraction or purification treatments. The assay is performed in about 30 min, requiring 150 nL of starting sample and it enables the detection of down to 15 bacterial cells. With respect to traditional culture techniques, the speed, the simplicity and the cheapness of this procedure allow an effective monitoring of microbial cells during food-making and processing. 相似文献
42.
Reynaldo Moreno Giovanna M. Aita Lee Madsen Dina L. Gutierrez Shaomian Yao Barry Hurlburt Suzanne Brashear 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2013,88(5):948-957
BACKGROUND: Microalgae, with both high biomass productivity and oil content, are regarded as attractive candidates for the production of alternative biodiesel as well as for CO2 biofixation. In the present study, four microalgal strains native to southeastern Louisiana's waters were isolated and identified to evaluate their potential for the production of biodiesel. Selected strains were identified through genomic DNA in sequencing of either 16S rRNA or 18S rRNA genes followed by lipid and fatty acid content characterization and quantification. RESULTS: High correlation was found with known nucleotide sequence identities at 98% with Sellaphora pupula, and 99% with Synechococcus sp., Chlorella sorokiniana, Scenedesmus abundans, and Chlorella vulgaris (control). The fatty acid profiles of these organisms changed when using 5% CO2 aeration. Total fatty acids (TFA) decreased from 20.63 to 17.62, 54.83 to 24.4, and 29.82 to 23.99 g kg?1 in Synechococcus sp., Sellaphora pupula and Chlorella sorokiniana, respectively. TFA increased from 14.14 to 31.49 and 15.14 to 47.52 g kg?1 dry biomass in Scenedesmus abundans and Chlorella vulgaris (control), respectively. CONCLUSION: Chlorella sorokiniana, with a lower C18:3 and the highest biomass yield at 5% CO2 aeration, was found to be the best candidate for biodiesel production. © 2012 Society of Chemical Industry 相似文献
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Jordan A. Doumanov Christina Zeitz Paloma Dominguez Gimenez Isabelle Audo Abhay Krishna Giovanna Alfano Maria Luz Bellido Diaz Veselina Moskova-Doumanova Marie-Elise Lancelot José-Alain Sahel Emeline F. Nandrot Shomi S. Bhattacharya 《International journal of molecular sciences》2013,14(7):15121-15140
46.
Strains of Clostridium butyricum that produce botulinal toxin type E have been implicated in outbreaks of foodborne botulism in China, India, and Italy, yet the conditions that are favorable for the growth and toxinogenesis of these strains remain to be established. We attempted to determine the temperatures and pH levels that are most conducive to the growth of and toxin production by the six strains of neurotoxigenic C. butyricum that have been implicated in outbreaks of infective and foodborne botulism in Italy. The strains were cultured for 180 days on Trypticase-peptone-glucose-yeast extract broth at various pHs (4.6, 4.8, 5.0, 5.2, 5.4, 5.6, and 5.8) at 30 degrees C and at various temperatures (10, 12, and 15 degrees C) at pH 7.0. Growth was determined by checking for turbidity; toxin production was determined by the mouse bioassay. We also inoculated two foods: mascarpone cheese incubated at 25 and 15 degrees C and pesto sauce incubated at 25 degrees C. The lowest pH at which growth and toxin production occurred was 4.8 at 43 and 44 days of incubation, respectively. The lowest temperature at which growth and toxin production occurred was 12 degrees C, with growth and toxin production first being observed after 15 days. For both foods, toxin production was observed after 5 days at 25 degrees C. Since the strains did not show particularly psychrotrophic behavior, 4 degrees C can be considered a sufficiently low temperature for the inhibition of growth. However, the observation of toxin production in foods at room temperature and at abused refrigeration temperatures demands that these strains be considered a new risk for the food industry. 相似文献
47.
Tablets containing two different doses of ibuprofen are realized. The first possesses very fast release kinetics, while the second has slow and linear release kinetics. This allows drug to produce a therapeutic effect quickly and to maintain it for a long time with only one administration unit. Such tablets are obtained by compression of a mixture of two very different kinds of granulates: an ibuprofen-starch granulate and an ibuprofen-Eudragit RS microsphere granulate. Specific proportions of mixtures of them give the described result after compression at particular tablet hardnesses. 相似文献
48.
Giovanna Loredana La Torre Marcello SaittaFortunato Vilasi Teresa PellicanòGiacomo Dugo 《Food chemistry》2006
Phenolic compounds in Sicilian wines were directly detected using an HPLC with a PDA detector coupled on-line with a MS system equipped with Electrospray Ionisation (ESI) source operated in the negative-ion mode and a quadrupole mass analyzer. In this work, MS spectra were recorded at different voltage, to obtain structural elucidations in addition to molecular mass informations. The different response of the compounds identified has been also evaluated. MS characteristics of cis- and trans-piceid were determined on the basis of the response obtained with the ESI interface. 相似文献
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Falcieri E Burattini S Bortul R Luchetti F Tabellini G Tazzari PL Cappellini A Cocco L Martelli AM 《Microscopy research and technique》2003,62(3):192-200
Two distinct types of cell death have been described: apoptosis and necrosis. However, it is becoming increasingly clear that the differences between these two types are far less numerous than initially thought. Morphological analyses might provide important information to distinguish apoptotic from necrotic samples. We recently reported that in necrotic, but not apoptotic, HL-60 human myeloid leukaemia cells, the nuclear protein topoisomerase IIalpha concentrated in nucleoli. In order to ascertain whether or not this phenomenon was restricted to a peculiar cell type or could be detected also in cells of lymphoid lineage, we performed an investigation aimed at defining the localization of topoisomerase IIalpha in apoptotic and necrotic Jurkat human T lymphoblastoid cells. Immunofluorescence staining demonstrated that topoisomerase IIalpha was excluded from the condensed chromatin of apoptotic cells, whereas in necrotic cells it was localized in discrete nuclear dots. Immuno-electron microscopy analysis showed that topoisomerase IIalpha was undetectable in nucleoli of normal and apoptotic cells, whereas it was present in the nucleolus of necrotic cells irrespectively of the type of inducer used (ethanol, H(2)O(2), HgCl(2)). Taken together, our findings identify topoisomerase IIalpha as a potential morphological marker useful to discriminate between apoptotic and necrotic cells. 相似文献