Investigation of platelet activation and calcium store release by a topical hemostatic xerogel dressing for improved blood clotting

Adequate topical hemostatic dressings are required as first-line approach for reduction of uncontrolled hemorrhage, a leading cause of mortalities. Platelets play a major role during blood clotting to prevent hemorrhage during injuries. Here, we demonstrate the mechanisms activated through protease activating receptor 1 (PAR1), a platelet membrane protein in response to a porous composite xerogel dressing incorporating SiNPs (size 122 ± 10 nm) and calcium (2.5 mM), characterized by scanning electron microscopy–energy dispersive x-ray spectroscopy and Fourier transform infrared spectroscopy. The composite displayed 13.9-fold improved blood clotting index in comparison to commercial dressing. The composite displayed increased platelet aggregation due to development of well-formed pseudopodia as compared to bare xerogel, SFLLRN (a thrombin mimic), adenosine di-phosphate (a platelet activator), and heparin (a thrombin inhibitor). Further, PAR1 gene was significantly upregulated in model A549 epithelial cell line (1.2-fold) and human platelets (1.4-fold). The composite enhanced calcium release and its extrusion in A549 cells. Upregulation of PAR1 on the platelet surface and calcium release are crucial for platelet shape change and aggregation. The data indicate that xerogel composite containing SiNPs and calcium enhanced blood clotting through activation of PAR1. Such dressings can provide a potential hemostatic solution to reduce blood loss, disability, and mortality during surgery and trauma care.     

On the triggering mechanism for the metal–insulator transition in thin film VO<Subscript>2</Subscript> devices: electric field versus thermal effects

Vanadium dioxide (VO₂) has been shown to undergo an abrupt electronic phase transition near 70 °C from a semiconductor to a metal, with an increase in dc conductivity of over three orders of magnitude, making it an interesting candidate for advanced electronics as well as fundamental research in understanding correlated electron systems. Recent experiments suggest that this transition can be manifested independent of a structural phase transition in the system, and that it can be triggered by the application of an electric field across the VO₂ thin film. Several experiments that have studied this behavior, however, also involve a heating of the VO₂ channel by leakage currents, raising doubts about the underlying mechanism behind the transition. To address the important question of thermal effects due to the applied field, we report the results of electro-thermal simulations on a number of experimentally realized device geometries, showing the extent of heating caused by the leakage current in the “off” state of the VO₂ device. The simulations suggest that in a majority of the cases considered, Joule heating is insufficient to trigger the transition by itself, resulting in a typical temperature rise of less than 10 K. However, the heating following a field-induced transition often also induces the structural transition. Nevertheless, for certain devices, we identify the possibility of maintaining the field-induced high conductivity phase without causing the structural phase transition: an important requirement for the prospect of making high-speed switching devices based on VO₂ thin film structures. Such electronically driven transitions may also lead to novel device functionalities including ultra-fast sensors or gated switches incorporating ferroelectrics.     

Cloning and Sequence Analysis of the estA gene encoding enzyme for producing (R)-β-acetylmercaptoisobutyric acid from Pseudomonas aeruginosa 1001

The estA gene encoding the enzyme that catalyzes the production of (R)-β-acetylmercaptoisobutyric acid from (R,S)-ester from Pseudomonas aeruginosa 1001, was cloned in Escherichia coli and its nucleotide sequence was determined, revealing the presumed open reading frame encoding a polypeptide of 316 amino acid residues (948 nucleotides). The overall A+T and C+G compositions were 32.59% and 67.41%, respectively. The amino acid sequence of the estA gene product showed a significant similarity with that of the triacylglycerol lipase from Psychrobacter immobilis (38% identity), triacylglycerol lipase from Moraxella sp. (36% identity), and two forms of carboxyl esterases from Acinetobacter calcoaceticus (17% and 17% identities). The deduced amino acid sequences have a pentapeptide consensus sequence, G-X-S-X-G, having an active serine residue, and another active site, dipeptides H-G, located at 70–100 amino acids upstream of the G-X-S-X-G consensus sequence.     

Structure and morphology of small diameter electrospun aramid fibers

Very small diameter fibers of poly(p-phenylene terephthalamide) (PPTA) ranging in diameter from 40nm to a few hundreds of nanometers were made by the electrospinning process. The fibers have a circular cross-section, are birefringent and stable at temperatures greater than 400°C. Electron diffraction patterns obtained from both as-spun and annealed fibers show meridional and equatorial reflections demonstrating order in the material. The crystal structure and morphology of the fibers were characterized using bright and dark field electron microscopy, electron diffraction and atomic force microscopy.     

Effect of N+ ion implantation on the corrosion behaviour of Ti—6Al—7Nb and Ti—5Al—2Nb—1Ta orthopaedic alloys in Hanks solution

Ti—6Al—7Nb and Ti—5Al—2Nb—1Ta alloys were implanted with N⁺ ions with an ion energy of 75 keV at a dose rate of 1 × 10¹⁷ and 1 × 10¹⁸ ions cm^–2. Open circuit potential (OCP) and potentiodynamic cyclic polarization measurements were carried out on the titanium alloys in Hanks solution to assess their corrosion resistance. The tendency for repassivation is higher in the case of implanted alloys than in untreated alloys owing to the formation of stable passive films. The impedance data showed a decrease in the double layer capacitance and an increase in the charge transfer resistance of the treated alloys. Nitrogen ion implanted Ti—6Al—7Nb was found to be more corrosion resistant than implanted Ti—5Al—2Nb—1Ta alloy.     

Life Cycle Cost of Electricity Generation from Biomass Gasifier

India is having more than 500,000 villages of which about 85% have been electrified. But as per RGGVY （Rejiv Gandhi Grammen Vidyutikaran Yojana）, the rate of village electrification is much lower as household connectivity has been fairly low. The rest 15% villages and a larger proportion of households have to be electrified. Villages have been a major concern as cost of electrification is fairly high. The most favored alternative to any kind of users is generation of electricity from diesel generating sets and renewable sources of energy. But the capital cost of renewable energy equipments is fairly high. Gradually, there is a reduction in the prices of these systems due to availability of better technological options and they are becoming competitive to grid electricity. In this paper, an attempt has been made to calculate the cost of production of electricity from stand-alone, off-grid devices biomass gasifiers （both dual fuel and pure gas type） and compare with that of diesel generating sets by using the concept of LCC （life cycle costing） and Homer software. It is found that the cost of per unit electricity generation （kWh） has been always the lowest in comparison to diesel generating sets even if the price of biomass increases to some extent.     

3D printed meat and the fundamental aspects affecting printability

Three-dimensional (3D) printing, one of the forms of additive manufacturing, has become a popular trend worldwide with a wide range of applications including food. The technology is adaptable and meets foods nutritional and sensory needs allowing meat processing to reach a sustainable level, technology addressing the food requirement of the ever-increasing population and the fast-paced lifestyle by reducing food preparation time. By minimizing food waste and the strain on animal resources, technology can help to create a more sustainable economy and environment. This review article discusses the 3D printing process and various 3D printing techniques used for food printing, such as laser powder bed fusion, inkjet food printing, and binder jetting, a suitable 3D technique used for meat printing, such as extrusion-based bioprinting. Moreover, we discuss properties that affect the printability of meat and its products with their applications in the meat industry, 3D printing market potential challenges, and future trends.     

Effect of system parameters on size distribution of 304 stainless steel particles produced by electrical discharge mechanism

An electrical discharge machine (EDM) can be used to produce Uranium Carbide (UC) microspheres. This material is useful for dispersed nuclear fuel fabrication. To obtain empirical data about the process parameters for UC modeling, 304 stainless steel (304 SS) was used to produce microspheres. Particle size effects were analyzed via three parameters; arc intensity, dielectric medium, and pulse width. Results indicate that the size and yield of the particles are dependent on all three parameters. The yield of larger particles increased with increasing arc current. Particle size distribution decreased with decreasing pulse width.