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61.
在3G牌照正式发放之前,我们仍然需要为新一轮大规模网络建设继续做准备。3G网络和现有2G网络如何互连互通,数据用户如何在3G网络和2G网络之间进行无缝连接,如何使系统网络方便地演进到IMS构架,这些问题都需要大家思考。一个经济有效且易于演进的网络结构,将对运营商顺利开展新一代移动业务,快速抢占发展的制高点,起到相当关键的作用。  相似文献   
62.
High level expression of recombinant human tumour necrosis factor β (rh TNF-β) in Escherichia coli results in the formation of two portions of protein, namely soluble active protein and insoluble protein which is inactive and aggregates in the form of inclusion bodies (IBs). In this study, a procedure for purification and renaturation of rh TNF-β from inclusion bodies has been designed and verified experimentally with a product purity of more than 90% and a recovery of about 30%. The procedure includes washing of IBs with specific wash buffer (Triton X-100/EDTA/lysozyme/PMSF), their solubilization with 8 mol dm?3 alkaline urea, purification with ion-exchange columns, refolding with renaturation buffer and finally concentration and desalination with an ultrafiltration membrane. The characteristics of the renatured protein were identical with those of purified protein from the soluble fraction as demonstrated by (1) SDS-PAGE, (2) cytotoxic activity on mouse L929 cells, (3) N-terminal amino acid sequence, and (4) gel filtration chromatography.  相似文献   
63.
The effects of α‐form and β‐form nuclei on polymorphic morphology of poly(butylene adipate) (PBA) upon recrystallization from the molten state up to various Tmax values were examined by differential scanning calorimetry (DSC), wide‐angle X‐ray diffraction (WAXD) and polarized light microscopy (PLM). In this study, PBA with complex melting and polymorphism behaviour was used as a model for examining different types and extents of residual nuclei. As the PBA initially containing the sole α‐crystal was brought to a molten state of various Tmax, the extents of trace α‐form crystal nuclei varied and were dependent on Tmax. Furthermore, it did not matter whether, initially, the PBA contained α‐ or β‐form crystals (or both) because only a single type of α‐nuclei could be left upon treatment to the molten liquid state at Tmax. Therefore, only the α‐crystal in PBA had ‘memory capacity’ in the molten liquid state while the β‐crystal did not. This was so because the latter had been completely transformed into the solid state prior to being heated into a liquid. PBA crystallized before α‐nuclei could be packed into α‐crystal, regardless of the crystallization temperature (Tc). For recrystallization from molten PBA without any nuclei, the crystalline polymorphism was correspondingly influenced by Tc. Copyright © 2005 Society of Chemical Industry  相似文献   
64.
One of the most interesting decision problems is how to select the most reliable design from among k competing designs. Under a Type II censoring plan, this paper constructs an MLR (modified likelihood ratio) rule associated with a simple algorithm to compute the sample size, number of failures and critical value which are called for by this rule. Besides, the performance of this selection rule was subject to several criteria to compare with the intuitive selection rule. It is seen that the MLR selection rule is better than the intuitive selection rule.  相似文献   
65.
As the result of vibration emission in air, a machine sound signal carries important information about the working condition of machinery. But in practice, the sound signal is typically received with a very low signal-to-noise ratio. To obtain features of the original sound signal, uncorrelated sound signals must be removed and the wavelet coefficients related to fault condition must be retrieved. In this paper, the blind source separation technique is used to recover the wavelet coefficients of a monitored source from complex observed signals. Since in the proposed blind source separation (BSS) algorithms it is generally assumed that the number of sources is known, the Gerschgorin disk estimator method is introduced to determine the number of sound sources before applying the BSS method. This method can estimate the number of sound sources under non-Gaussian and non-white noise conditions. Then, the partial singular value analysis method is used to select these significant observations for BSS analysis. This method ensures that signals are separated with the smallest distortion. Afterwards, the time-frequency separation algorithm, converted to a suitable BSS algorithm for the separation of a non-stationary signal, is introduced. The transfer channel between observations and sources and the wavelet coefficients of the source signals can be blindly identified via this algorithm. The reconstructed wavelet coefficients can be used for diagnosis. Finally, the separation results obtained from the observed signals recorded in a semi-anechoic chamber demonstrate the effectiveness of the presented methods .  相似文献   
66.
Diabetes mellitus is a common disease. It affects multiple organ systems. Adverse effects of hyperglycemia on infection, fracture healing, and bone remodeling have been recently reported. This study was conducted to evaluate the clinical and radiographic results of 93 total hip arthroplasties in 78 consecutive patients with diabetes. All femoral components were cemented using contemporary cementing techniques. Prophylactic antibiotics were given in each case. The mean follow-up period was 4.1 years (range, 2-6.5 years). Ninety-six percent of the hips were rated excellent or good. Radiolucencies were observed in only 3.7% of the stems, while 22.2% of the cups showed radiolucencies. There was a 4% revision incidence. There was no postoperative infection in this series--a distinct improvement from previously reported series. However, complications remained high at 24.3%. The most frequent complication was urinary tract infection (14.2%). The most serious complication was myocardial infarction. The authors believe total hip arthroplasty can be safely performed in patients with diabetes, provided that adequate medical and follow-up evaluations are performed. The medium-term clinical and radiographic follow-up evaluations are not inferior to reported series in patients without diabetes.  相似文献   
67.
A novel and efficient approach is proposed to calculate the dispersions of the guided modes of the photonic-crystal fibers (PCFs). Based on the vector boundary-element method (VBEM), the surface integral equations for the first and second derivatives of the propagation constants with respect to the wavelength are explicitly derived. Compared with the three-point finite-difference approach, which needs to solve and search three effective indexes near the interested wavelength, this approach can determine the dispersions of the PCFs by only solving one effective index at this wavelength based on the derived formulations. This novel approach saves over 60% computing time without losing the accuracy. Based on this approach, a novel four-ring PCF is designed by optimizing only three geometrical parameters to achieve the nearly zero ultra-flattened dispersion property. Compared with previously presented dispersion-flattened PCFs, the design procedure for the four-ring structure could be more efficient and easier because relatively lesser parameters need to be optimized.  相似文献   
68.
The purpose of this paper is twofold. The first objective is to examine the core resources that make environmental management system (EMS) a potential basis of sustainable competitive advantage from the resource-based view of the firm. The second objective is to investigate the set of core resources from the contingency perspective. Particularly, experience in implementing other manufacturing systems and the stage of EMS implementation were tested as the contingency factors. The proposed model and its related hypotheses were tested using structural equation modeling on a large-scale database consisting of 1453 respondents. The results showed that it is the tacit resources within an EMS, in the form of top management team's strategic perception, cross-functional cooperation, and environmentally responsible suppliers, which help explain firmspsila operational performance. The findings provided the general principles in investment decisions of EMS. However, the results also demonstrated that the pattern of the core resources varies across firms with different experience in other manufacturing systems and at different stages of EMS implementation. As a result, there is no standard bundle of critical resources for each firm. Rather, firms need to tailor their resource investment decisions in light of these factors beyond the general principles.  相似文献   
69.
This letter presents a primary investigation into developing a compact and low-loss bandpass filter using novel folded-waveguide resonators with a footprint reduction. A slot coupling between adjacent resonators is introduced, which is characterized using full-wave electromagnetic simulations and verified experimentally. Two two-pole folded-waveguide resonator filters of this type have been designed, fabricated and tested. Simulation and measurement results are presented to validate the design and to show the advantages of this type of filter.  相似文献   
70.
黎虹 《江西化工》2008,(4):168-170
本法采用碳酸钡、氟化锂、石墨粉作缓冲剂,用锑做铅锌的标.用铪作铌的内标,用钯作铍、铬、镧、锶、铜的内标。采用小孔电极,交流电弧做为激发光源,同时测定铅、锡、锌、铍、铬、镧、铌、铜、锶等九个元素。分析数据基本稳定.能达到土壤样品的检出限要求。  相似文献   
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