Enhanced Ca2+ Entry Sustains the Activation of Akt in Glucose Deprived SH-SY5Y Cells

The two crucial cellular insults that take place during cerebral ischemia are the loss of oxygen and loss of glucose, which can both activate a cascade of events leading to neuronal death. In addition, the toxic overactivation of neuronal excitatory receptors, leading to Ca²⁺ overload, may contribute to ischemic neuronal injury. Brain ischemia can be simulated in vitro by oxygen/glucose deprivation, which can be reversible by the re-establishment of physiological conditions. Accordingly, we examined the effects of glucose deprivation on the PI3K/Akt survival signaling pathway and its crosstalk with HIF-1α and Ca²⁺ homeostasis in SH-SY5Y human neuroblastoma cells. It was found that glucose withdrawal decreased HIF-1α protein levels even in the presence of the ischemia-mimicking CoCl_2. On the contrary, and despite neuronal death, we identified a strong activation of the master pro-survival kinase Akt, a finding that was also confirmed by the increased phosphorylation of GSK3, a direct target of p-Akt. Remarkably, the elevated Ca²⁺ influx recorded was found to promptly trigger the activation of Akt, while a re-addition of glucose resulted in rapid restoration of both Ca²⁺ entry and p-Akt levels, highlighting the plasticity of neurons to respond to ischemic challenges and the important role of glucose homeostasis for multiple neurological disorders.     

Electrochemical modeling and study of copper deposition from concentrated ammoniacal sulfate solutions

A fundamental investigation of the electrolytic deposition of copper from concentrated aqueous ammoniacal solutions has been carried out based on the thermodynamic analysis of the system Cu–NH₃–H₂O. The speciation of copper vs. pH and redox potential was modeled in high ionic strength solutions, in which the activity coefficients of the system species were estimated according to the Modified Bromley's Methodology. The electrochemical behavior of the redox system Cu(0)/Cu(I)/Cu(II) in concentrated aqueous ammoniacal solutions was studied at pH = 9.5 and the cathodic reactions in these solutions were determined. It was found that metallic copper was formed under strongly reductive redox conditions, while under mildly reductive to mildly oxidative conditions the cuprous di-ammine complex species dominate. Under highly oxidative conditions the cupric tetra-ammine complex species predominated. According to the theory and results, the cathodic deposition of copper from concentrated aqueous ammoniacal solutions proceeds in a two-step reduction mechanism. The cupric ammine species are first reduced to cuprous di-ammine, which in turn is reduced to metallic copper. The electrochemical experiments revealed that copper deposition over time follows a sigmoid-type curve, verifying the two-step mechanism. The main feature of these sigmoid curves was the presence of an induction period with negligible copper deposition, followed by an acceleration period where the copper deposition rate gradually increased. By increasing the applied cell voltage, the induction period was significantly reduced or disappeared.     

Hardware Architectures for the Efficient Implementation of Multi-Service Broadband Access and Multimedia Home Networks

In multimedia applications, the stringent requirements for balancing transmission capacity, flexible service provisioning and cost reduction lead the manufactures to provide highly integrated System-on Chip (SoC) solutions. This paper analyzes the application of high-bandwidth-networking SoCs to improve on the cost efficiency of multimedia service distribution in home networks. We present a case study, where we utilize the inherent protocol processing capabilities and high bandwidth interfaces of a modern network processor, scaled down to match the performance targets and low cost requirements of the home networking environment. An efficient, low cost Residential Gateway architecture results by mapping the home services onto the processing and memory blocks of this SoC. This revised version was published online in September 2006 with corrections to the Cover Date.     

Inhalation of 30% nitrous oxide impairs people's learning without impairing people's judgments of what will be remembered.

Many hypotheses have been proposed to account for the effects of nitrous oxide on memory, with one emerging possibility being that it has a global effect on memory-related functioning. This possibility was explored by examining the effects of nitrous oxide on memory performance and on the accuracy of people's judgments about their memory performance. Participants inhaled 30% nitrous oxide or a placebo gas while items were studied and while judgments were made about the likelihood of recall for each item. Next, all participants inhaled the placebo during paired-associate recall. Although administration of nitrous oxide during study impaired recall, it did not affect the predictive accuracy of the metacognitive judgments. These results provide pharmacological evidence for a distinction between memory and metamemory. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Royal jelly fatty acids modulate proliferation and cytokine production by human peripheral blood mononuclear cells

Royal jelly (RJ) fatty acids have recently been shown to possess various pharmacological and biological activities. In this work, we studied the immunomodulatory effects of 10-hydroxy-trans-2-decenoic acid (10-HDA) and 3,10-dihydroxy-decanoic acid (3,10-DDA), isolated from RJ, using a model of phytohaemagglutinin-activated human peripheral blood mononuclear cells (PBMCs). We showed that higher concentrations (500 μM) of both fatty acids inhibited the proliferation of PBMCs, and the process was followed by a decrease in the production of interleukin-2 (IL-2). 10-HDA at the concentration of 500 μM inhibited the production of IL-1β and tumor necrosis factor-α by stimulated PBMCs, whereas the same dose of 3,10-DDA had no effect on the levels of these cytokines. Regarding T helper (Th) cytokine profile, higher concentration of 10-HDA, in contrast to the lower one (50 μM), inhibited both Th1 and Th2 response, whereas Th17 response was not significantly modulated, as judged by the levels of interferon-γ, IL-5 and IL-17A in culture supernatants, respectively. Lower concentration of 3,10-DDA stimulated Th1 and Th17 responses and inhibited IL-10 production, whereas the higher dose augmented the Th2 response. In conclusion, our results showed a significant, dose-dependent, immunomodulatory effect of RJ fatty acids in vitro, which was also associated with their structure.     

Technological Evolution of Historic Structural Mortars

Mortars are among the first building materials used in constructions, even from prehistoric times （8th millennium BC）. Their study reveals a great source of information regarding the evolution of their technological characteristics and application techniques, the availability and exploitation of raw materials, as well the wider socio-economic aspects of each era. The aim of this paper is to comparatively evaluate the analysis results from approximately 1,000 structural mortar samples taken from various monuments and historic buildings of Greece, dated from the Hellenistic period, until the beginning of the 20th century. The analysis focused in the determination of their physico-mechanical and chemical properties, such as porosity, apparent specific gravity, mechanical strength, aggregates type and granulometry and chemical composition. Through the results＇ evaluation, significant remarks can be made upon the evolution of the raw materials used （binding system, aggregates, additives）, as well as regarding the final properties of historic structural mortars. It is concluded that hydrated lime was the main binding agent used for a long-lasting period of 2.5 millenniums, while mixed type binding systems based on lime and natural pozzolan were systematically used for producing durable mortars, resistant to humidity. In any case, it seems that ancient masons were fully aware of the significant role of mortars in constructions and were capable of exploiting the available raw materials and application techniques to the maximum.     

Reconstruction procedure for 3D micro X-ray absorption fine structure

A new approach for chemical speciation in stratified systems using 3D Micro-XAFS spectroscopy is developed by combining 3D Micro X-ray Fluorescence Spectroscopy (3D Micro-XRF) and conventional X-ray Absorption Fine Structure Spectroscopy (XAFS). A prominent field of application is stratified materials within which depth-resolved chemical speciation is required. Measurements are collected in fluorescence mode which in general lead to distorted spectra due to absorption effects. Developing a reliable reconstruction algorithm for obtaining undistorted spectra for superficial and in-depth layers is proposed and validated. The developed algorithm calculates the attenuation coefficients of the analyte for the successive layers facilitating a new spectroscopic tool for three-dimensionally resolved nondestructive chemical speciation.     

Improvements on “IP – IAPP”: A fast IP handoff protocol for IEEE 802.11 wireless and mobile clients

One of the most critical issues in introducing Wireless LAN (WLAN) real-time and delay sensitive applications, such as Voice over IP (VoIP), is guaranteeing IP service continuation during inter-subnet Basic Service Set (BSS) transitions. Even though WLANs offer very high channel bandwidth, they exhibit long network-layer handoff latency. This is a restraining factor for mobile clients using interactive multimedia applications such as VoIP or video streaming. In a previous work, we presented a novel fast and efficient IP mobility solution, called “IP-IAPP”, which offers constant IP connectivity to the 802.11 mobile users and successfully preserves their ongoing sessions, even during subnet handoffs (fast recovery of active connections). It is an 802.11-dependent IP mobility solution, which accelerates the network reconfiguration phase after subnet handoffs and significantly reduces the IP handoff latency. It restores L3 connectivity almost simultaneously to the L2 connectivity after a subnet handoff, due to a zero-delay movement detection method. As a result, even the most demanding next generation WLAN applications such as Voice over WLAN (VoWLAN) suffer insignificant disruption. In this paper we present an improved version of the IP-IAPP mobility mechanism (new optimized protocol procedures). Certain extensions have also been incorporated to the initial proposal, for the provision of more advanced services: (a) secure inter-AP IP-IAPP communications, (b) zero patching on the clients s/w, and (c) support of clients which use a dynamic IP address. Performance measurements out of further and more complex testing verify that the proposed method outperforms other existing mobility solutions, and still introduces the lesser imperative amendments to the existing 802.11 wireless LAN framework. Ioanna F. Samprakou received her B. Eng in Computer Engineering and Informatics in 2000 and her MSc. in the same area in 2003 from the University of Patras, Greece. She is currently a Ph.D canditate at the University of Patras. She has joined Atmel SA in 2002, where she is a Senior Wireless System Eng at the System Concept and Design group. She specializes in wireless technologies, and mobile communications, and holds a patent in the field of IP mobility. She has led teams in developing wireless 802.11 products such as Wi-Fi APs, STAs, and VoIP phones. Previously she has worked for the Research & Academic Computer & Technology Institute of Patras (RACTI) as an R&D computer engineer. She is a member of the Technical Chamber of Greece. Christos J. Bouras obtained his Diploma and PhD from the Computer Science and Engineering Department of Patras University (Greece). He is currently an Associate Professor in the above department. Also he is a scientific advisor of Research Unit 6 in Research Academic Computer Technology Institute (CTI), Patras, Greece. His research interests include Analysis of Performance of Networking and Computer Systems, Computer Networks and Protocols, Telematics and New Services, QoS and Pricing for Networks and Services, e – learning, Networked Virtual Environments and WWW Issues. He has extended professional experience in Design and Analysis of Networks, Protocols, Telematics and New Services. He has published 200 papers in various well-known refereed conferences and journals. He is a co-author of 7 books in Greek. He has been a PC member and referee in various international journals and conferences. He has participated in R&D projects such as RACE, ESPRIT, TELEMATICS, EDUCATIONAL MULTIMEDIA, ISPO, EMPLOYMENT, ADAPT, STRIDE, EUROFORM, IST, GROWTH and others. Also he is member of, experts in the Greek Research and Technology Network (GRNET), Advisory Committee Member to the World Wide Web Consortium (W3C), IEEE Learning Technology Task Force, IEEE Technical Community for Services Computing WG 3.3 Research on Education Applications of Information Technologies and W 6.4 Internet Applications Engineering of IFIP, Task Force for Broadband Access in Greece, ACM, IEEE, EDEN, AACE and New York Academy of Sciences. Theodore E. Karoubalis. received his B. Eng in Computer Engineering and Informatics in 1992 and his Ph.D. in the same area in 1996 from the University of Patras, Greece. He has joined ATMEL Hellas SA at 1998. Since 1998 he is the Manager of PSLi software dpt. and since 2002 he is the manager of System and Concepts dpt. His interests include systems on chip, embedded applications, wireless systems etc. He is a member of IEEE and the Technical Chamber of Greece.     

Mixtures of Gaussian Wells: Theory, Computation, and Application

A primary challenge in unsupervised clustering using mixture models is the selection of a family of basis distributions flexible enough to succinctly represent the distributions of the target subpopulations. In this paper we introduce a new family of Gaussian Well distributions (GWDs) for clustering applications where the target subpopulations are characterized by hollow [hyper-]elliptical structures. We develop the primary theory pertaining to the GWD, including mixtures of GWDs, selection of prior distributions, and computationally efficient inference strategies using Markov chain Monte Carlo. We demonstrate the utility of our approach, as compared to standard Gaussian mixture methods on a synthetic dataset, and exemplify its applicability on an example from immunofluorescence imaging, emphasizing the improved interpretability and parsimony of the GWD-based model.