实施项目群和项目组合管理——从标准到执行

如今，人们再也不会将项目视为特殊的活动而将其置于组织真空中来执行。经过多年研究、传播及行业应用，项目群和项目组合管理的相关概念最终被诸多标准化机构采用，并形成了自己的知识体系。     

Structural Characteristics of the 5′-Terminal Region of Mouse p53 mRNA and Identification of Proteins That Bind to This mRNA Region

A mouse model has often been used in studies of p53 gene expression. Detailed interpretation of functional studies is, however, hampered by insufficient knowledge of the impact of mouse p53 mRNA’s structure and its interactions with proteins in the translation process. In particular, the 5′-terminal region of mouse p53 mRNA is an important region which takes part in the regulation of the synthesis of p53 protein and its N-truncated isoform Δ41p53. In this work, the spatial folding of the 5′-terminal region of mouse p53 mRNA and its selected sub-fragments was proposed based on the results of the SAXS method and the RNAComposer program. Subsequently, RNA-assisted affinity chromatography was used to identify proteins present in mouse fibroblast cell lysates that are able to bind the RNA oligomer, which corresponds to the 5′-terminal region of mouse p53 mRNA. Possible sites to which the selected, identified proteins can bind were proposed. Interestingly, most of these binding sites coincide with the sites determined as accessible to hybridization of complementary oligonucleotides. Finally, the high binding affinity of hnRNP K and PCBP2 to the 5′-terminal region of mouse p53 mRNA was confirmed and their possible binding sites were proposed.     

Analysis of emptiness (lacunarity) as a measure of the degree of space filling and of the internal structure of a set

The study presents a comparative evaluation of emptiness (lacunarity) in model structures with different types of inhomogeneity of dispersed particle distributions. The research on emptiness (lacunarity) as a measure of the degree of space filling and, at the same time, of the internal characterization of a set has been based on the optimal covering and gliding-box methods.     

Evaluation of various integration algorithms for transient inelastic response of shear walls

Inelastic dynamic response of building walls made of reinforced concrete is considered by using various explicit and implicit algorithms. The numerical examples show the computational efficiency of algorithms for different low and high walls.     

Aspects of dSpace in contemporary design

Computer dependent design space is illustrated with the recent international competition project for Kowloon Arts Center. The spatial condition of design resulted from the deployment of Euclidian geometry and use of the nonrational, complex surfaces. Another aspect of computer dependent space was rooted in the contemporary work situation where architects located on the three different continents collaborated in distributed mode over the internet. The resulting project confirms the creative potential of the above aspects of the digital space in contemporary design.     

Multifractal detection of overlaps based on a stereometric analysis of a fracture surface: Assumptions

The paper presents theoretical assumptions of an analysis of stereometric files for the purpose of the detection of the occurrence of overlaps. The measured data of a fracture surface were obtained using a profilographometer. An algorithm has been developed based on the probability of the occurrence of maximum Euclidean distances in the investigated areas of the stereometric image. An analysis of real fractures and overlaps [Stach S, Roskosz S, Cwajna J, Cybo J. Graphic visualization of overlaps occurring in sintered carbides' fractures, Materials Characterization 2006-this issue. doi:10.1016/j.matchar.2006.01.007. [1]] has corroborated a high effectiveness of multifractal detection.     

Changes in structure of psoas major and minor and semitendinosus muscles of calves, heifers and cows during post-mortem ageing

Changes occurring during post-mortem ageing in structure of psoas major and minor (PM) and semitendinosus (ST) muscles of calves, heifers and cows were observed. Samples from muscles taken 3 h after slaughter and after 6 and 12 days of storage at the temperature of 4?°C were analysed using light and transmission electron microscopy. Three hours after slaughter muscle fibres were close to each other and sarcomeres showed normal structure of A- and I-bands, M-lines and Z-disks. The space between myofibrils and sarcolemma and also between single myofibrils in muscle fibres increased during storage. Structure of sarcomeres underwent continuous degradation, length of sarcomeres increased due to enlargement of I-bands accompanied by Z-disk degradation. During ageing structural changes in myofibrils took place faster, were more intensive in muscles of younger animals and were more extensive and faster in PM than in ST muscles.     

Basic principles of thermal degradation and thermal stabilization of poly(vinyl chloride). Synergism of stabilizer action

Changes in the quantities of active stabilizing components during thermal aging of plasticized poly(vinyl chloride) containing a mixture of calcium and zinc stearates were analyzed. A mathematical model of the stabilization process by using the stabilizer mixture and a computer program for analyzing the process of PVC thermal degradation after the beginning of HCl evolution are given.     

Calculation of phase equilibria for AISI M2 high-speed steel

Thermo-calc computations have been made for the iron-rich corner of the system Fe-Cr-Mo-W-V-C to obtain phase equilibria for AISI M2 high-speed steel (German designation S-6-5-2). The calculations describe the main transformation temperatures of this steel with good accuracy. The phases which are present during austenitization and their volume fractions are also correctly accounted for. The solidification sequence of the M2 steel, and in particular the occurrence of M₂C in the ledeburitic eutectic, can be explained if the segregation of alloying elements and carbon in the interdendritic spaces is taken into account. The secondary hardening precipitates can be described in satisfactory approximation on the basis of local two-phase equilibria between each carbide and the matrix, indicating that their compositions are essentially determined by the nucleation process.