Design of a novel MRI compatible manipulator for image guided prostate interventions

This paper reports a novel remotely actuated manipulator for access to prostate tissue under magnetic resonance imaging guidance (APT-MRI) device, designed for use in a standard high-field MRI scanner. The device provides three-dimensional MRI guided needle placement with millimeter accuracy under physician control. Procedures enabled by this device include MRI guided needle biopsy, fiducial marker placements, and therapy delivery. Its compact size allows for use in both standard cylindrical and open configuration MRI scanners. Preliminary in vivo canine experiments and first clinical trials are reported.     

Review of translocations detected by FISH for retrospective biological dosimetry applications

Several European laboratories have combined their research efforts to arrive at a consensus view on using fluorescence in situ hybridisation (FISH) for retrospective dosimetry. The aim of this review is to report these views and to highlight some areas where further work is needed. Translocations in the stable cells should be measured only in the cells that contain the full complement of the painted material. Two-way and one-way translocations should be combined with equal weight. The control level of translocations has a strong dependence on age, which has now been measured and the system has been calibrated. In conclusion, the technique works and a lifetime dose to the bone marrow from low-linear energy transfer radiation of 0.5 Gy above normal background levels can be measured for any individual. The main application is considered to provide an independent verification of lifetime doses to individuals who might form a part of an epidemiological study.     

Computer-controlled microcirculatory support system for endothelial cell culture and shearing

Endothelial cells (ECs) lining the inner lumen of blood vessels are continuously subjected to hemodynamic shear stress, which is known to modify EC morphology and biological activity. This paper describes a self-contained microcirculatory EC culture system that efficiently studies such effects of shear stress on EC alignment and elongation in vitro. The culture system is composed of elastomeric microfluidic cell shearing chambers interfaced with computer-controlled movement of piezoelectric pins on a refreshable Braille display. The flow rate is varied by design of channels that allow for movement of different volumes of fluid per variable-speed pump stroke. The integrated microfluidic valving and pumping system allowed primary EC seeding and differential shearing in multiple compartments to be performed on a single chip. The microfluidic flows caused ECs to align and elongate significantly in the direction of flow according to their exposed levels of shear stress. This microfluidic system overcomes the small flow rates and the inefficiencies of previously described microfluidic and macroscopic systems respectively to conveniently perform parallel studies of EC response to shear stress.     

Geometric superresolution by code division multiplexing

In many highly resolved optical systems the resolution is limited not by the optics but by the CCD's nonzero pixel size. As a result, overall resolution is decreased. Here we propose a novel approach to enhancing resolution beyond the limit set by the CCD's pixels. This method does not involve additional mechanical elements, such as those used for microscans. In this scheme neither the CCD nor additional elements are moved. The geometric superresolving procedure is based on code-division multiplexing, with all its inherent benefits, such as relative noise immunity to single-tone interference. A setup is proposed for coherent and incoherent illumination, with slight modifications for the latter. A theoretical analysis of the setup is presented and compared with empirical results. This scheme is shown to enhance one-dimensional image resolution with the use of only a simple mask that doubles image resolution. This method can easily be expanded to two-dimensional images and to resolution-enhancement factors greater than 2.     

Uniform large-area x-ray imaging at 9 keV using a backlit pinhole

The development and application of point backlighting at high x-ray energies is an essential step in diagnosing radiation-driven experiments. The point-backlighting technique provides uniform backlighter irradiance over a large field of view. This technique circumvents the large laser energy required for area backlighters at energies of 9 keV and above. We present the results of a Zn 9 keV point-backlighter source using the technique of pinhole aperturing to define the source size and hence the resolution. Details of the design and application of this technique to an undriven gold-walled hohlraum are described.     

High-speed dynamic speckle interferometry: phase errors due to intensity, velocity, and speckle decorrelation

The recently developed technique of high-speed phase-shifting speckle interferometry combined with temporal phase unwrapping allows dynamic displacement fields to be measured, even for objects containing global discontinuities such as cracks or boundaries. However, when local speckle averaging is included, small phase errors introduced at each time step are accumulated along the time axis, yielding total phase values that depend strongly on the speckle rereference rate. We present an analysis of the errors introduced in the phase evaluation by three sources: intensity errors, velocity errors, and speckle decorrelation. These errors are analyzed when they act both independently and together, for the most commonly used phase-shifting algorithms, with computer-generated speckle patterns. It is shown that, in a controlled out-of-plane geometry, errors in the unwrapped phase map that are due to speckle decorrelation rise as the time between rereferencing events is increased, whereas those due to intensity and velocity errors are reduced. It is also shown that speckle decorrelation errors are typically more important than the intensity and velocity errors. These results provide guidance as to the optimal speckle rereferencing rate in practical applications of the technique.