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101.
Displacement fields have been calculated around 1/2[111] screw dislocations with various types of core structures in bcc metals. Three types of cores are studied: two types of polarized cores with large and small extensions of the displacement fields and an isotropic core. The difference in the displacement along the [111] direction Δuz from that for the elastic solution has been evaluated for each type of dislocation. In the outside of the core region, the Δuz values are close to zero along the six
directions and the regions with Δuz>0 and Δuz<0 are alternately arranged, lying between those directions. Appreciable difference in Δuz has been detected between the polarized cores and the isotropic core up to large distance from the core region. The defocus convergent-beam electron diffraction patterns have been calculated for the dislocations with the incident beam parallel to the dislocation line. Winding and spiral features have been shown in the higher-order Laue zone (HOLZ) lines for the dislocated structures, which have been confirmed by a preliminary experiment. In addition, small shifts of the HOLZ lines have been shown by the calculation between the polarized cores and the isotropic one. 相似文献
102.
Keiichi Moriguchi Michiya Utsumi Norikazu Ohno 《Microscopy research and technique》2013,76(12):1284-1291
Based on its histochemical properties, the secretory portion of the hamster submandibular gland has been classified as seromucous cells. The presence of endogenous peroxidase (PO) reaction was shown in the nuclear envelope, cisternae of endoplasmic reticulum and Golgi apparatus. The 3,3′‐diaminobenzidene, tetrahydrochloride (DAB) method revealed bipartite secretory granules containing a PO‐positive dense core surrounded by a less dense halo in these cells. In the present investigation, serous and mucous‐like cells were found in resin‐embedded semi‐thin sections of the DAB‐reacted hamster submandibular gland. These sections were already on glass slides for routine light microscopic observations, therefore electron microscopic analysis could be unrealizable. We then used reflectance‐mode confocal laser scanning microscopy to visualize additional sites of PO activity as detected in these sections. Using this approach, we found mucous cells with PO activity‐negative secretory granules and seromucous cells with PO activity‐positive spot‐like secretory granules of the regular sublingual gland most frequently adjacent to the serous cells with typical electron‐dense secretory granules. These cells clearly differ from the seromucous cells with bipartite secretory granules and the granular duct cells with typical electron‐dense secretory granules of the hamster submandibular gland. Additionally, secretory endpieces of the ectopic sublingual gland‐like tissue empty into the duct of the hamster submandibular gland lobule. Thus, our findings suggest that a mass of sublingual gland tissue extends into the hamster submandibular gland during its development, and PO may be synthesized and secreted into the same duct. Microsc. Res. Tech. 76:1284–1291, 2013. © 2013 Wiley Periodicals, Inc. 相似文献
103.
Keiichi Onish Youngwoo Kim Goro Obinata Kazunori Hase 《Journal of Mechanical Science and Technology》2013,27(5):1341-1350
We investigated impedance perception characteristics of humans in the man-machine interface. Sensibility or operational feel about physical properties of machine dynamics is obtained through perception process. We evaluated the impedance perception characteristics of humans who are operating a mechanical system, based on extended Scheffe’s subjective evaluation method in full consideration of the influence of impedance level, impedance difference, experiment order, individual difference and so on. Constant method based quantitative evaluation was adopted to investigate the influence of motion frequency and change of the impedance on human impedance perception characteristics. Experimental results indicate that humans perceive impedance of mechanical systems based on comparison process of the dynamical characteristics of the systems. The proposed method can be applied to quantify the design requirement of man-machine interface. The effectiveness of the proposed method is verified through experimental results. 相似文献
104.
105.
Keiichi Tomishige Yutaka Furusawa Yoshiki Ikeda Mohammad Asadullah Kaoru Fujimoto 《Catalysis Letters》2001,76(1-2):71-74
CeO2–ZrO2 solid solution catalysts are very effective for the selective synthesis of dimethyl carbonate from methanol and CO2. The activity was much dependent on the calcination temperature. The higher the calcination temperature, the higher the activity of the catalyst for DMC formation, though the BET surface area is lower on the catalyst calcined at higher temperature. 相似文献
106.
Koganesawa Nozomi; Aizawa Tomoyasu; Masaki Kazuo; Matsuura Atsushi; Nimori Taisuke; Bando Hisanori; Kawano Keiichi; Nitta Katsutoshi 《Protein engineering, design & selection : PEDS》2001,14(9):705-710
Expression systems of human and silkworm lysozymes were constructedusing the methylotrophic yeast Pichia pastoris as a host. Theleader sequence and its prepro peptide of 相似文献
107.
Ayaka Koma Shunichi Asai Chikashi Minemura Sachi Oshima Takashi Kinoshita Naoko Kikkawa Keiichi Koshizuka Shogo Moriya Atsushi Kasamatsu Toyoyuki Hanazawa Katsuhiro Uzawa Naohiko Seki 《International journal of molecular sciences》2021,22(18)
We newly generated an RNA-sequencing-based microRNA (miRNA) expression signature of head and neck squamous cell carcinoma (HNSCC). Analysis of the signature revealed that both strands of some miRNAs, including miR-139-5p (the guide strand) and miR-139-3p (the passenger strand) of miR-139, were downregulated in HNSCC tissues. Analysis of The Cancer Genome Atlas confirmed the low expression levels of miR-139 in HNSCC. Ectopic expression of these miRNAs attenuated the characteristics of cancer cell aggressiveness (e.g., cell proliferation, migration, and invasion). Our in silico analyses revealed a total of 28 putative targets regulated by pre-miR-139 (miR-139-5p and miR-139-3p) in HNSCC cells. Of these, the GNA12 (guanine nucleotide-binding protein subunit alpha-12) and OLR1 (oxidized low-density lipoprotein receptor 1) expression levels were identified as independent factors that predicted patient survival according to multivariate Cox regression analyses (p = 0.0018 and p = 0.0104, respectively). Direct regulation of GNA12 and OLR1 by miR-139-3p in HNSCC cells was confirmed through luciferase reporter assays. Moreover, overexpression of GNA12 and OLR1 was detected in clinical specimens of HNSCC through immunostaining. The involvement of miR-139-3p (the passenger strand) in the oncogenesis of HNSCC is a new concept in cancer biology. Our miRNA-based strategy will increase knowledge on the molecular pathogenesis of HNSCC. 相似文献
108.
Yoshifuru Mitsui Keiichi Koyama Kazuo Watanabe 《Science and Technology of Advanced Materials》2009,10(1)
A system was developed measuring x-ray powder diffraction in high magnetic fields up to 5 T and at temperatures from 283 to 473 K. The stability of the temperature is within 1 K over 6 h. In order to examine the ability of the system, the high-field x-ray diffraction measurements were carried out for Si and a Ni-based ferromagnetic shape-memory alloy. The results show that the x-ray powder diffraction measurements in high magnetic fields and at high temperatures are useful for materials research. 相似文献
109.
Kazuhiko Kato Takeshi Hibino Keiichi Komoto Seijiro Ihara Shuji Yamamoto Hideaki Fujihara 《Solar Energy Materials & Solar Cells》2001,67(1-4)
Authors have evaluated the life cycle of a thin-film CdS/CdTe PV module to estimate the energy payback time (EPT) and the life-cycle CO2 emissions of a residential rooftop PV system using the CdS/CdTe PV modules. The primary energy requirement for producing 1 m2 of the CdS/CdTe PV module was similar to a-Si PV module at annual production scale of 100 MW. EPT was calculated at 1.7–1.1 yr, which was much shorter than the lifetime of the PV system and similar to that of a-Si PV modules. The life-cycle CO2 emissions were also estimated at 14–9 g-C/kWh, which was less than that of electricity generated by utility companies. 相似文献
110.
A combined technique of the rapid freezing, freeze substitution–fixation method and the osmium–DMSO-osmium method was devised. By this combined method we clearly observed the architecture of intracellular components in three dimensions. Morphological characteristics were generally similar to those of tissue prepared by the osmium–DMSO-osmium method but different in some respects. Mucigen droplets in intestinal goblet cells, for example, appeared as separated spheres, while in specimens prepared by chemical fixation they were observed as a mass of fused droplets. In the Golgi complex, all cisternae were extremely flat, although they usually dilated on the cis side after chemical fixation. Particles on the mitochondrial tubules of liver cells were well distinguished. They were mushroom shaped, as are those observed by negative staining. The combined method, that is, the rapid freezing, osmium–DMSO-osmium method, is thought to be effective for studying the true structure of intracellular components by scanning electron microscopy. 相似文献