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71.
We describe the electrochemical detection of DNA methylation through the direct oxidation of both 5-methylcytosine (mC) and cytosine (C) in 5'-CG-3' sequence (CpG) oligonucleotides using a sputtered nanocarbon film electrode after digesting a longer CpG oligonucleotide with endonuclease P1. Direct electrochemistry of the longer CpG oligonucleotides was insufficient for obtaining the oxidation currents of these bases because the CG rich sequence inhibited the direct oxidation of each base in the longer CpG oligonucleotides, owing to the conformational structure and its very low diffusion coefficient. To detect C methylation with better quantitativity and sensitivity in the relatively long CpG oligonucleotides, we successfully used an endonuclease P1 to digest the target CpG oligonucleotide and yield an identical mononucleotide 2'-deoxyribonucleoside 5'-monophosphate (5'-dNMP). Compared with results obtained without P1 treatment, we achieved 4.4 times higher sensitivity and a wider concentration range for mC detection with a resolution capable of detecting a subtle methylated cytosine difference in the CpG oligonucleotides (60mer). 相似文献
72.
Keisuke Nakajima Saori Oka Takashi Tanikawa Yoko Nemoto-Sasaki Naoki Matsumoto Hiroki Ishiguro Yoichiro Arata Takayuki Sugiura Atsushi Yamashita 《International journal of molecular sciences》2022,23(18)
We previously reported that lysophosphatidylinositol (LPI) functions as an endogenous agonist of GPR55, a novel cannabinoid receptor. However, the physiological roles of LPI-GPR55 have not yet been elucidated in detail. In the present study, we found that LPI induced morphological changes in GPR55-expressing HEK293 cells. LPI induced the cell rounding of GPR55-expressing HEK293 cells but not of empty-vector-transfected cells. LPI also induced the activation of small GTP-binding protein RhoA and increased stress fiber formation in GPR55-expressing HEK293 cells. The inhibition of RhoA and Rho kinase ROCK by the C3 exoenzyme and the ROCK inhibitor reduced LPI-induced cell rounding and stress fiber formation. These results clearly indicated that the LPI-induced morphological changes and the assembly of the cytoskeletons were mediated through the GPR55-RhoA-ROCK pathway. 相似文献
73.
Keisuke TanizawaTetsuo Sekiya Shigeo OhshioHiroki Akasaka Hidetoshi Saitoh 《Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms》2011,269(3):313-317
The development of an intense X-ray source using backscattered X-ray produced using an advanced electrode configuration is described. The electrodes were composed of field emitters deposited on a wire mounted on a perforated plate as the cathode and a copper plate as the anode. Electrons from these emitters collided with the copper plate and X-ray was generated at collision points. The backscattered X-ray in the direction normal to the electron trajectory through a hole in the anode escaped from the vacuum chamber through a beryllium window. Continuous and characteristic X-rays were detected at an applied voltage lower than that of a conventional X-ray source from 3.0 to 9.4 kV, respectively. Moreover, the X-ray dosage measured with a survey meter reached 0.95 mSv/h at 5.0 kV of applied voltage. The transmission images of three types of material used as an X-ray source for the X-ray imaging system indicate three advantages; low power consumption, focal point controllable by adjusting applied voltage, and photographable motion picture of X-ray transmission. 相似文献
74.
Keisuke Fujita Kohji Higuchi Kazushi Nakano Koji Matsushita Tatsuyoshi Kajikawa Fumiho Chino 《Artificial Life and Robotics》2009,14(3):433-439
There is a need for robust current control of a pulse width modulation (PWM) power amplifier whose transient response characteristics
do not deteriorate with extensive load changes and/or direct-current power supply voltage changes. In this article, we propose
a digital robust controller with bumpless mode switching to control the current of a PWM power amplifier to satisfy the demands
and extend the range of an inductive load width. It is necessary to measure the value of the load in order to implement this
bumpless mode switching automatically depending on the load range. Therefore, a method of estimating the inductive load is
shown. The bumpless mode switching is automatically performed by estimating an inductive value without specifying the value
of the inductive load beforehand. That is, the value of the inductive load is estimated during the DDC execution, and the
control mode is automatically switched bumplessly according to this estimated value. A digital controller equipped with inductance
estimation and bumpless mode switching is realized by a DSP. Some experiments show that the digital controller with the proposed
bumpless mode switching can satisfy larger specifications. 相似文献
75.
Shirai T Matsuzaki K Kuzumoto M Nagahisa K Furusawa C Shioya S Shimizu H 《Journal of Bioscience and Bioengineering》2006,102(5):413-424
Precise metabolic flux analysis (MFA) by gas chromatography-mass spectrometry (GC-MS) and computer calculation was performed, and the consistency of the estimated results was verified by independently performed nuclear magnetic resonance (NMR) analysis. The precise estimation of flux by the integration method of the mass isotopomer signal, defined as the coefficient of variance (CV) of multiple determination, was investigated, and the results estimated using different data sets with the same magnitude of error were confirmed. The CV of multiple determinations was sufficiently small to discuss and compare the fluxes of a metabolic pathway. The estimated fluxes using the GC-MS data were cross-validated with the NMR data that were independently measured and not used for MFA. The developed method was successfully applied to the MFA of the growth phase of two different glutamate-producing coryneform bacteria, Corynebacterium glutamicum and C. efficiens. The difference in the growth rate between these two bacterial species was discussed while considering the results of MFA, including forward and backward (exchange) fluxes. 相似文献
76.
Asakawa M Takayama H Beppu R Miyazawa K 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2005,46(6):246-250
To assess levels of shellfish intoxication by the paralytic shellfish poison (PSP)-producing dinoflagellate Alexandrium tamarense, potential health risks to human shellfish consumers and the possible need for regulatory intervention, yearly variations of maximum cell density of this species were examined from 1993 to 2004 in Kure Bay and Kaita Bay, which are located within Hiroshima Bay, Hiroshima Prefecture, Japan. The seawater temperature was determined concomitantly. In Kure Bay, maximum concentrations of 1,400 and 1,300 cells/mL at 0 and 5 m depths were observed on 21 and 24 April 1997. In Kaita Bay, remarkably high concentrations above 1,000 cells/mL of A. tamarense were observed in two out of three years investigated. These facts suggest that the environment in both bays is favorable for the propagation of A. tamarense. The temperature range at which the natural population of A. tamarense blooms was generally from 12 to 16 degrees C. Four strains (ATKR-94, -95, -97 and -01) from Kure Bay and one strain (ATKT-97) from Kaita Bay were established. The strain ATKR-94, cultured in modified SW-2 medium at 15 degrees C for 15 days, showed a specific toxicity of 33.8 x 10(-6) MU/cell. The toxins in all five strains exist almost exclusively as beta-epimers (C2 (PX2 or GTX8), GTX3, dcGTX3 and GTX4), which accounted for 54.9 to 73.0 mol% of the total. The corresponding a-epimers (C1 (PX1 or epi-GTX8), GTX2, dcGTX2 and GTX1) accounted for 6.0 to 28.9 mol%. The toxin profiles of ATKR-97 and ATKT-97 were characterized by unusually high proportions of low-potency sulfocarbamoyl toxin, which comprised 62.4 and 68.2 mol%, respectively, of total toxins. In the toxic bivalves, the low-toxicity sulfocarbamoyl components, major components of A. tamarense, were present in amounts of only a few percent, suggesting that in vivo conversion of PSP occurs after ingestion. A comparison of the toxin profiles of the causative dinoflagellate and contaminated bivalves showed that PSP components exist in the bivalves in the form of alpha-epimers, presumably owing to accumulation or storage of the toxins. 相似文献
77.
78.
Seismic electromagnetic (EM) phenomena have been observed at many laboratories with specially designed systems. Recently we can utilize radio receivers, personal computers, and communication systems with higher performances and reasonable cost. It is possible to observe seismic EM phenomena not only by specially designed systems but also by a simple observation system integrated by these devices and systems. We have created an observation system for seismic EM phenomena with them in the very high frequency (VHF) band, from 76 MHz to 90 MHz, assigned for FM broadcasting exclusive use in Japan. We are surrounded by many EM waves, such as broadcasting, communication usage, and man‐made noises. Thus, we have newly developed the dual frequency observation method. In this paper, we describe an observation method that can identify whether a received signal is a broadband EM wave or an artificial FM radio wave. Next, the observation results for broadband EM waves from galactic noise and solar flares are presented. Then, the observation results for FM radio waves reflected from sporadic E layer and the Leonid meteors are shown. Finally, the observation results for the detected broadband EM waves associated with the Geiyo earthquake are described. © 2006 Wiley Periodicals, Inc. Electr Eng Jpn, 155(4): 36–44, 2006; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20350 相似文献
79.
The key words for the future telecommunications are personalization, multi-media services and high accessibility to the network. These trends naturally lead to radio media application to access links. In this sense, this paper proposes an advanced wireless access system with a capacity supporting broadband video and data services.Concept of the system can be defined as a new radiocommunication category with advantages of both fixed and mobile systems.The system is designed to transport ATM-based signals using SHF (or EHF) bands to wireless terminals, interconnected with optical fibers at the network side. The basic system configuration is presented as well as examples of system parameters.For radio aspects the maximum service coverages of the radio base stations are calculated based on availability consideration. Preferred frequency bands for actual operation are also discussed. 相似文献
80.