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121.
Cdc7 kinase is essential for initiation of DNA replication. Cdc7(-/-) mouse embryonic stem (ES) cells are non-viable but their growth can be rescued by an ectopically expressed transgene (Cdc7(-/-)tg). Here we report that, despite the normal growth capability of Cdc7(-/-)tg ES cells, the mice with the identical genetic background exhibit growth retardation. Concomi tantly, Cdc7(-/-)tg embryonic fibroblasts (MEFs) display delayed S phase entry and slow S phase progression. Furthermore, spermatogenesis of Cdc7(-/-)tg mice is disrupted prior to pachytene stage of meiotic prophase I. The impairment in spermatogenesis correlates with the extremely low level of Cdc7 protein in testes, and is rescued by introducing an additional allele of transgene, which results in increase of Cdc7 expression. The increased level of Cdc7 also recovers the growth of Cdc7(-/-)tg MEFs and mice, indicating that the developmental abnormalities of Cdc7(-/-)tg mice are due to insufficiency of Cdc7 protein. Our results indicate the requirement of a critical level of a cell-cycle regulator for mouse development and provide genetic evidence that Cdc7 plays essential roles in meiotic processes in mammals. 相似文献
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This newsletter provides information on past and upcoming events related to the IEEE Computer Society's Test Technology Technical Council and the test community. 相似文献
124.
The secondary effluent from municipal plants in Korea generally represents higher BOD with lower SS. Therefore, more soluble forms of organics and NH4-N need to be removed to improve its effluent for reuse. In this study reuse possibility of secondary effluent and CSO (combined sewer overflow) using BAF (biological aerated filter) was evaluated. The tertiary application with 1.2 h EBCT, SS, BOD and COD showed stable concentrations less than 1.3, 1.3 and 6.2 mg/L, respectively. Nitrogen could be nitrified even at 7 degrees suggesting BAF can be used for a water reuse method as well as an effective add-on facility in cold regions. However, BAF was not stable with CSO application at increased flow rates suggesting CSO must be equalised prior to application. Disinfection was necessary even during normal weather conditions. 相似文献
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Kyung-Won Lee Eung Soo Kim Hee Cheon No Chul-Hwa Song 《Nuclear Engineering and Design》2006,236(9):1003-1012
We experimentally investigate the de-entrainment of droplets by inertial impaction on an array of vertical rods in an air-droplet mixture flow. The de-entrainment efficiencies are measured for a single rod, for a single row of rods, and for a multi-row of rods. We investigate the effects of the droplet mass flux (0.5–5.4 kg/m2 s), the droplet Weber number (3000–8000), the air velocity (0–6 m/s), the rod geometry, and the surface roughness on the de-entrainment, and the rod diameter-to-pitch ratio effect on the de-entrainment. The results for a single rod show that the de-entrainment efficiency decreases slightly as the droplet mass flux increases; however, in our experimental ranges, there is negligible dependence on the droplet Weber number, the air velocity, and the surface roughness. The rod geometry affects the de-entrainment efficiency. The results for a single row of rods show that the existence of neighboring rods promotes de-entrainment due to droplet splashing, and we develop a correlation to show the effect of diameter-to-pitch ratio on the de-entrainment. Using information on the de-entrainment efficiencies of a single rod and those of a single row of rods, we propose a correlation that predicts the de-entrainment efficiency for a multi-row of rods with a staggered array. The RMS errors of the correlation from the de-entrainment efficiencies experimentally obtained are within 13.5%. 相似文献
130.
Zone 3 of a distance relay is used to provide the remote backup protection in case of the failure of the primary protection. However, the risk of maloperation under stressed conditions such as heavy loading, voltage, and transient instability is quite high. Zone 3 is used in combination with the derivatives of the voltage and current, etc. to prevent maloperation. At times, the impedance characteristics that restrict the tripping area of relay are used to avoid maloperation due to load encroachment. This work presents a novel zone 3 scheme based on combining the steady-state components (i.e., 60 Hz) and the transient components (TCs) using a state diagram that visualizes the sequence of studies that emanate from the sequence of events. The simulation results show that the novel zone 3 distance relay elements using the proposed method operate correctly for the various events. 相似文献