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排序方式: 共有299条查询结果,搜索用时 15 毫秒
71.
Kavan Motazedi Yaser Khojasteh Salkuyeh Ian J. Laurenzi Heather L. MacLean Joule A. Bergerson 《International Journal of Hydrogen Energy》2021,46(41):21274-21288
Alternative hydrogen production technologies are sought in part to reduce the greenhouse gas (GHG) emissions intensity compared with Steam Methane Reforming (SMR), currently the most commonly employed hydrogen production technology globally. This study investigates hydrogen production via High Temperature Steam Electrolysis (HTSE) in terms of GHG emissions and cost of hydrogen production using a combination of Aspen HYSYS® modelling and life cycle assessment. Results show that HTSE yields life cycle GHG emissions from 3 to 20 kg CO2e/kg H2 and costs from $2.5 to 5/kg H2, depending on the system parameters (e.g., energy source). A carbon price of $360/tonne CO2e is estimated to be required to make HTSE economically competitive with SMR. This is estimated to potentially decrease to $50/tonne CO2e with future technology advancements (e.g., fuel cell lifetime). The study offers insights for technology developers seeking to improve HTSE, and policy makers for decisions such as considering support for development of hydrogen production technologies. 相似文献
72.
Biosynthesis of Colabomycin E,a New Manumycin‐Family Metabolite,Involves an Unusual Chain‐Length Factor
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Dr. Kateřina Petříčková Dr. Stanislav Pospíšil Dr. Marek Kuzma Dr. Tereza Tylová Dr. Michal Jágr Petr Tomek Dr. Alica Chroňáková Eva Brabcová Dr. Ladislav Anděra Dr. Václav Krištůfek Dr. Miroslav Petříček 《Chembiochem : a European journal of chemical biology》2014,15(9):1334-1345
Colabomycin E is a new member of the manumycin‐type metabolites produced by the strain Streptomyces aureus SOK1/5‐04 and identified by genetic screening from a library of streptomycete strains. The structures of colabomycin E and accompanying congeners were resolved. The entire biosynthetic gene cluster was cloned and expressed in Streptomyces lividans. Bioinformatic analysis and mutagenic studies identified components of the biosynthetic pathway that are involved in the formation of both polyketide chains. Recombinant polyketide synthases (PKSs) assembled from the components of colabomycin E and asukamycin biosynthetic routes catalyzing the biosynthesis of “lower” carbon chains were constructed and expressed in S. aureus SOK1/5‐04 ΔcolC11–14 deletion mutant. Analysis of the metabolites produced by recombinant strains provided evidence that in both biosynthetic pathways the length of the lower carbon chain is controlled by an unusual chain‐length factor supporting biosynthesis either of a triketide in asukamycin or of a tetraketide in colabomycin E. Biological activity assays indicated that colabomycin E significantly inhibited IL‐1β release from THP‐1 cells and might thus potentially act as an anti‐inflammatory agent. 相似文献
73.
Modi K Fazio R Pascazio S Vedral V Yuasa K 《Philosophical transactions. Series A, Mathematical, physical, and engineering sciences》2012,370(1976):4810-4820
We construct a quantumness witness following the work of Alicki & van Ryn (AvR). We reformulate the AvR test by defining it for quantum states rather than for observables. This allows us to identify the necessary quantities and resources to detect quantumness for any given system. The first quantity turns out to be the purity of the system. When applying the witness to a system with even moderate mixedness, the protocol is unable to reveal any quantumness. We then show that having many copies of the system leads the witness to reveal quantumness. This seems contrary to the Bohr correspondence, which asserts that, in the large-number limit, quantum systems become classical, whereas the witness shows quantumness when several non-quantum systems, as determined by the witness, are considered together. However, the resources required to detect the quantumness increase dramatically with the number of systems. We apply the quantumness witness for systems that are highly mixed but in the large-number limit that resembles nuclear magnetic resonance (NMR) systems. We make several conclusions about detecting quantumness in NMR-like systems. 相似文献
74.
The growth of graphene during Cu-catalyzed chemical vapor deposition was studied using 12CH4 and 13CH4 precursor gasses. We suggest that the growth begins by the formation of a multilayer cluster. This seed increases its size but the growth speed of a particular layer depends on its proximity to the copper surface. The layer closest to the substrate grows fastest and thus further limits the growth rate of the upper layers. Nevertheless, the growth of the upper layers continues until the copper surface is completely blocked. It is shown that the upper layers can be removed by modification of the conditions of the growth by hydrogen etching. 相似文献
75.
Václav Trojan Tomáš Vyhnánek Ondřej Štastník Eva Mrkvicová Jan Mareš Ladislav Havel 《Journal für Verbraucherschutz und Lebensmittelsicherheit》2016,11(3):259-264
Delivery of foreign DNA is still a very controversial issue, especially in relation to genetically modified organisms. It is not necessary to use genetically modified feed to verify the possible transfer of DNA from food into the body. In this study, we evaluated the possible transfer of DNA from wheat forage (of single component diet) into the blood of three different organisms. DNA isolation kits were used to isolate total DNA from blood and feed. Blood was taken for analysis from rats (after 23 days feeding wheat), broilers (15 days), and carp (34 days). Housekeeping genes were used to verify blood samples (rats: glyceraldehyde-3-phosphate dehydrogenase/GAPDH/, broilers: growth hormone gene/GCH/and carp: small ribosomal subunit/40S/). We used GAPDH and actin to determine whether DNA from wheat was transferred into animal blood. No purple and common wheat DNA fragments were detected in the blood of any animal tested, suggesting no transfer of the tested wheat DNA into the body. Various contents of anthocyanins in the feeding variants had no effect on the transfer of wheat DNA into the blood of animals. Our results correspond to some studies that have not proved transfer DNA from genetically modified feed into the target organism. 相似文献
76.
The absorption and reflection spectra of a Grätzel-type solar cell were evaluated in terms of heat losses. Using these experimental data, the temperature distribution inside the solar cell was calculated. The temperature-controlling process in this cell is the heat transfer from the cell to the environment. Assuming the environment temperature of 25°C, the natural air convection leads to the temperature of 46.9°C inside the TiO2 layer. Forced air convection causes the decrease, up to 30.1°C at the wind speed of 10 m s−1. Variations in the cell dimensions, except the active layer length, have only a small effect on the temperature field, compared to the variations in the heat transfer coefficients. 相似文献
77.
Krist P Vannucci L Kuzma M Man P Sadalapure K Patel A Bezouska K Pospísil M Petrus L Lindhorst TK Kren V 《Chembiochem : a European journal of chemical biology》2004,5(4):445-452
GlcNAc-coated glycodendrimers, which are polyvalent glycomimetics, display strong in vitro affinity for the rat natural killer cell protein-1A (NKR-P1A), a C-type lectin-like receptor of natural killer (NK) cells in rats, humans and some strains of mice. Administration of these compounds in vivo results in a substantial increase in the antitumour activity with involvement of the natural cell immunity. To clarify the in vitro and in vivo fate of these molecules, we synthesized labelled glycodendron analogues of the previously studied glycodendrimers. Labelling with fluorescent tags enabled the localization of the glycodendrons in white blood cells, tumours and other tissues by using different imaging techniques such as fluorescence and confocal microscopy. These studies are useful for probing the mechanism of action and fate of artificial ligands and the cell receptors involved. 相似文献
78.
Mirko M. Mili Ladislav A. Novak 《International Journal of Circuit Theory and Applications》1989,17(4):409-419
The graph-theoretic properties depending on the element types and their controllability but not on the particular form of constitutive relations of elements are considered. New concepts such as normal partition pair of the branch set, weak completeness and weak non-degeneracy are introduced and their properties are investigated in detail. These properties may then be used to formulate purely topological methods of analysing some dynamical aspects of nonlinear RLC networks. 相似文献
79.
Aldo Roda Patrizia Simoni Elida N Ferri Stefano Girotti Adriano lus Pavel Rauch Martin Poplstein Milan Pospisil Petr Pipek Igor Hochel Ladislav Fukal 《Journal of the science of food and agriculture》1999,79(1):58-62
An enzyme immunoassay was used to determine benzo[a ]pyrene (BaP) in smoked meat products and other samples of food and environmental origin. The method used has a detection limit (3 σ) of 0.1 μg kg−1 and a coefficient of variation less than 10%. The main aim of the study was to compare the possible influence of different smoking processes and packaging material on the amount of BaP deposited on smoked meat product, mainly different sausages. The lowest amount of BaP was found when smoke produced by steam in the indirect method smoking‐chamber was used. A slightly protective effect of polyamide casing was noted. © 1999 Society of Chemical Industry 相似文献
80.
Jana Fulne
kov Ladislav Dokldal Karolína Kolov Martina Nepor Dadejov Klra Prochzkov Sabina Gomelsk Martin Siv
k Kateina Adamusov Martin Ly
ka Vratislav Peska Martina Dvo
kov Eva Sýkorov 《International journal of molecular sciences》2022,23(1)
Telomerase, an essential enzyme that maintains chromosome ends, is important for genome integrity and organism development. Various hypotheses have been proposed in human, ciliate and yeast systems to explain the coordination of telomerase holoenzyme assembly and the timing of telomerase performance at telomeres during DNA replication or repair. However, a general model is still unclear, especially pathways connecting telomerase with proposed non-telomeric functions. To strengthen our understanding of telomerase function during its intracellular life, we report on interactions of several groups of proteins with the Arabidopsis telomerase protein subunit (AtTERT) and/or a component of telomerase holoenzyme, POT1a protein. Among these are the nucleosome assembly proteins (NAP) and the minichromosome maintenance (MCM) system, which reveal new insights into the telomerase interaction network with links to telomere chromatin assembly and replication. A targeted investigation of 176 candidate proteins demonstrated numerous interactions with nucleolar, transport and ribosomal proteins, as well as molecular chaperones, shedding light on interactions during telomerase biogenesis. We further identified protein domains responsible for binding and analyzed the subcellular localization of these interactions. Moreover, additional interaction networks of NAP proteins and the DOMINO1 protein were identified. Our data support an image of functional telomerase contacts with multiprotein complexes including chromatin remodeling and cell differentiation pathways. 相似文献