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排序方式: 共有279条查询结果,搜索用时 10 毫秒
81.
Impedance measurement of growth of lactic acid bacteria in the presence of nisin and lysozyme 总被引:1,自引:0,他引:1
Drahomíra?KozákováEmail author Jitka?Holubová Milada?Plocková Jana?Chumchalová Ladislav??urda 《European Food Research and Technology》2005,221(6):774-778
Effect of nisin (0.500 g/l), lysozyme (0.035 g/l) and the mix of nisin (0.500 g/l) and lysozyme (0.035 g/l) against two nisin-producing
lactococci strains, three non-nisin-producing lactococci strains and four lactobacilli strains with antimicrobial activity
was determined by the impedimetric method. None of the tested lactococci and lactobacilli strains were inhibited by lysozyme
at concentration of 0.035 g/l. Only the nisin-producing lactococci strains were resistant to nisin at 0.500 g/l, the other
lactococci and lactobacilli were inhibited by nisin at 0.500 g/l intensively. The mix of lysozyme and nisin displayed a comparable
effect to the nisin addition against the used lactococci strains and was very variable depending on the strain of tested lactobacilli.
The impedimetric method was shown to be convenient for screening the growth abilities of lactococci and lactobacilli in the
presence of antimicrobial components. 相似文献
82.
Two air-pollution-control (APC) residues--one from flue gas cooling with alkaline water and one from deionized water cooling--from secondary lead metallurgy were submitted to two different standardized short-term leaching protocols: US EPA toxicity characteristic leaching procedure (TCLP) and static leaching according to Czech/European norm EN 12457-2. The experimental procedure was coupled with detailed mineralogical investigation of the solid material (SEM, XRPD) and speciation-solubility calculations using the PHREEQC-2 geochemical code. Both types of residues were considered as hazardous materials exhibiting substantial leaching of Pb (up to 7130 mg/l) and other inorganic contaminants. However, the APC residue produced by flue gas cooling with alkaline water (sample B) exhibits more favourable leaching and environmental characteristics than that produced by simple deionised water cooling (sample A). At pH < 5, primary caracolite (Na3Pb2(SO4)3Cl) and potassium lead chloride (KCl.2PbCl2) are completely or partially dissolved and transformed to residual anglesite (PbSO4), cotunnite (PbCl2) and laurionite (Pb(OH)Cl). At pH 5-6, anglesite is still the principal residual product, whereas at pH > 6, phosgenite (PbCl2.PbCO3) became the dominant secondary phase. The results are consistent with the mineralogical and geochemical studies focused on acidic forest soils highly polluted by smelter emissions, where anglesite, as a unique Pb-bearing phase, has been detected. From the technological point of view, the mixing of APC residue with alkaline water, followed by an increase in the suspension pH and equilibration with atmospheric CO2, may be used to ensure the precipitation of less soluble Pb carbonates, which are more easily recycled in the Pb recovery process in the metallurgical plant. 相似文献
83.
Ladislav J. Kuera 《Journal of microscopy》1981,124(3):319-325
This report deals with the cutting of wood specimens for observation in the scanning electron microscope. Several cutting devices and types of knives are described and critically evaluated. 相似文献
84.
85.
86.
Von Ladislav Rosík 《大分子材料与工程》1971,16(1):209-220
In the ageing of industrial polybutadienes the effect of decomposition products of ZIEGLER–NATTA catalysts could not be distinguished from other Important factors. However, the additional introduction of Co, Ti or Ni compounds (components of the ZIEGLER-NATTA catalysts) has influenced the oxidation process. The effect of different acetylacetonates (Ti, Co, Ni, V, Al, Cc, Mn, Cu) on the oxidation of purified polybutadiene in presence or in absence of an antioxidant has further been studied. The apparent activation energies of oxidation have been estimated and possible mechanismus of the actions of metal compounds in the oxidation of polybutadiene have been proposed. 相似文献
87.
Kim C Erpelding TN Jankovic L Wang LV 《Philosophical transactions. Series A, Mathematical, physical, and engineering sciences》2011,369(1955):4644-4650
Clinical translation of photoacoustic (PA) imaging can be facilitated by integration with commercial ultrasound (US) scanners to enable dual-modality imaging. An array-based US scanner was modified for hand-held PA imaging. The performance was benchmarked in terms of signal-to-noise ratio (SNR), axial spatial resolution and sensitivity. PA images of a tube, filled with methylene blue (MB; approx. 30?mM) and placed at various depths in chicken tissue, were acquired. A 5?cm penetration depth was achieved with an 18.6?dB SNR using a laser fluence of 3?mJ?cm(-2), only one-seventh of the safety limit (20?mJ?cm(-2)). An axial resolution of approximately 400?μm was maintained at all imaging depths. The PA sensitivity to MB placed 2.3?cm deep in chicken tissue was less than 100?μM. Further, after intradermal injection of MB (approx. 30?mM), a rat sentinel lymph node was clearly identified in vivo, beneath a 3.8?cm thick layer of chicken breast. The accumulated concentration of MB in the node was estimated to be approximately 7?mM. The noise-equivalent sensitivities (approx. 2?cm depth) were 17 and 85?μM, ex vivo and in vivo, respectively. These results support the use of this PA system for non-invasive mapping and image-guided needle biopsy of sentinel nodes in breast cancer patients. 相似文献
88.
Maria Gazdova Radka Michalkova Martin Kello Maria Vilkova Zuzana Kudlickova Janette Baloghova Ladislav Mirossay Jan Mojzis 《International journal of molecular sciences》2022,23(20)
This study was focused on investigating the antiproliferative effects of chalcone hybrids in melanoma cancer cells. Among seven chalcone hybrids, the chalcone-acridine hybrid 1C was the most potent and was selected for further antiproliferative mechanism studies. This in vitro study revealed the potent antiproliferative effect of 1C via cell cycle arrest and apoptosis induction. Cell cycle arrest at the G2/M phase was associated with modulation of expression or phosphorylation of specific cell cycle-associated proteins (cyclin B1, p21, and ChK1), tubulins, as well as with the activation of the DNA damage response pathway. Chalcone 1C also induced apoptosis accompanied by mitochondrial dysfunction evidenced by a decrease in mitochondrial membrane potential, increase in Bax/Bcl-xL ratio and cytochrome c release followed by caspase 3/7 activation. In addition, increased phosphorylation of MAP kinases (Erk1/2, p38 and JNK) was observed in chalcone 1C-treated melanoma cells. The strong antiproliferative activities of this chalcone-acridine hybrid suggest that it may be useful as an antimelanoma agent in humans. 相似文献
89.
Ilona Hromadnikova Katerina Kotlabova Ladislav Krofta 《International journal of molecular sciences》2022,23(18)
We assessed the diagnostic potential of cardiovascular disease-associated microRNAs for the early prediction of gestational diabetes mellitus (GDM) in singleton pregnancies of Caucasian descent in the absence of other pregnancy-related complications. Whole peripheral venous blood samples were collected within 10 to 13 weeks of gestation. This retrospective study involved all pregnancies diagnosed with only GDM (n = 121) and 80 normal term pregnancies selected with regard to equality of sample storage time. Gene expression of 29 microRNAs was assessed using real-time RT-PCR. Upregulation of 11 microRNAs (miR-1-3p, miR-20a-5p, miR-20b-5p, miR-23a-3p, miR-100-5p, miR-125b-5p, miR-126-3p, miR-181a-5p, miR-195-5p, miR-499a-5p, and miR-574-3p) was observed in pregnancies destinated to develop GDM. Combined screening of all 11 dysregulated microRNAs showed the highest accuracy for the early identification of pregnancies destinated to develop GDM. This screening identified 47.93% of GDM pregnancies at a 10.0% false positive rate (FPR). The predictive model for GDM based on aberrant microRNA expression profile was further improved via the implementation of clinical characteristics (maternal age and BMI at early stages of gestation and an infertility treatment by assisted reproductive technology). Following this, 69.17% of GDM pregnancies were identified at a 10.0% FPR. The effective prediction model specifically for severe GDM requiring administration of therapy involved using a combination of these three clinical characteristics and three microRNA biomarkers (miR-20a-5p, miR-20b-5p, and miR-195-5p). This model identified 78.95% of cases at a 10.0% FPR. The effective prediction model for GDM managed by diet only required the involvement of these three clinical characteristics and eight microRNA biomarkers (miR-1-3p, miR-20a-5p, miR-20b-5p, miR-100-5p, miR-125b-5p, miR-195-5p, miR-499a-5p, and miR-574-3p). With this, the model identified 50.50% of GDM pregnancies managed by diet only at a 10.0% FPR. When other clinical variables such as history of miscarriage, the presence of trombophilic gene mutations, positive first-trimester screening for preeclampsia and/or fetal growth restriction by the Fetal Medicine Foundation algorithm, and family history of diabetes mellitus in first-degree relatives were included in the GDM prediction model, the predictive power was further increased at a 10.0% FPR (72.50% GDM in total, 89.47% GDM requiring therapy, and 56.44% GDM managed by diet only). Cardiovascular disease-associated microRNAs represent promising early biomarkers to be implemented into routine first-trimester screening programs with a very good predictive potential for GDM. 相似文献
90.
Ladislav J. Kucera 《Holz als Roh- und Werkstoff》1990,48(12):474-476
Ohne Zusammenfassung 相似文献