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101.
102.
Mairobi Persinal-Medina Sara Llames Manuel Chacn Natalia Vzquez Marta Pevida Ignacio Alcalde Sergio Alonso-Alonso Laura María Martínez-Lpez Jesús Merayo-Lloves lvaro Meana 《International journal of molecular sciences》2022,23(9)
The skin is the largest organ in the human body, comprising the main barrier against the environment. When the skin loses its integrity, it is critical to replace it to prevent water loss and the proliferation of opportunistic infections. For more than 40 years, tissue-engineered skin grafts have been based on the in vitro culture of keratinocytes over different scaffolds, requiring between 3 to 4 weeks of tissue culture before being used clinically. In this study, we describe the development of a polymerizable skin hydrogel consisting of keratinocytes and fibroblast entrapped within a fibrin scaffold. We histologically characterized the construct and evaluated its use on an in vivo wound healing model of skin damage. Our results indicate that the proposed methodology can be used to effectively regenerate skin wounds, avoiding the secondary in vitro culture steps and thus, shortening the time needed until transplantation in comparison with other bilayer skin models. This is achievable due to the instant polymerization of the keratinocytes and fibroblast combination that allows a direct application on the wound. We suggest that the polymerizable skin hydrogel is an inexpensive, easy and rapid treatment that could be transferred into clinical practice in order to improve the treatment of skin wounds. 相似文献
103.
Outi E. Mäkinen Reetta Kivelä Laura Nyström Mogens L. Andersen Tuula Sontag-Strohm 《Food chemistry》2012
The aim of this study was to determine the role of the formation of radicals and hydrogen peroxide in the viscosity loss of beta-glucan extracts from oat and barley. Radicals derived from endogenous H2O2 were formed spontaneously in all extracts. The decomposition of H2O2 by catalase had little or no effect on the viscosity, and the radical formation detected and measured by electron spin resonance (ESR) spectroscopy did not predict the shelf life of crude beta-glucan extracts. The addition of FeSO4 accelerated the radical formation and viscosity loss, revealing the sensitivity of the extract viscosity towards catalysts for oxidation. Using 50% cadoxen in the extracts slowed down the viscosity loss in all extracts but still led to a 10–70% decrease of the initial viscosities during 4 weeks of storage. The results suggest that the viscosity loss may be a result from multiple mechanisms including chemical reactions during storage as well as aggregation and conformational changes. 相似文献
104.
Sara S. Santos Alessandra Lorenzoni Luana M. Ferreira Juliane Mattiazzi Andréa I.H. Adams Laura B. Denardi Sydney H. Alves Scheila R. Schaffazick Letícia Cruz 《Materials science & engineering. C, Materials for biological applications》2013,33(3):1389-1394
Clotrimazole is a common choice for the treatment of vulvovaginal infections, but its low solubility and some side effects pose a challenge to its application. This work evaluated the feasibility to formulate clotrimazole-loaded cationic nanocapsules using Eudragit® RS100 and medium chain triglycerides as polymer and oily core, respectively, by the method of interfacial deposition of a preformed polymer. The physicochemical characteristics of nanocapsule formulations were evaluated at 0 day and 60 days after preparation. Particle size, zeta potential, polydispersity index, pH and drug content were stable during this period. In addition, nanocapsules were able to protect clotrimazole from photodegradation under UV radiation. By the dialysis bag diffusion technique, the nanosized formulations showed prolonged release of clotrimazole by anomalous transport and first order kinetics. A microbiological study was carried out by the microdilution method and showed that nanocapsules (mean size: 144 nm; zeta potential: + 12 mV) maintained the antifungal activity of clotrimazole against Candida albicans and Candida glabrata strains susceptible and resistant to fluconazole. 相似文献
105.
106.
The relationship between exposure to sexually objectifying music television, primetime television programs, fashion magazines, and social networking sites and the internalization of beauty ideals, self‐objectification, and body surveillance was examined among adolescent girls (N = 558). A structural equation model showed direct relationships between sexually objectifying media and the internalization of beauty ideals, and indirect relationships between sexually objectifying media and self‐objectification, and body surveillance through the internalization of beauty ideals. The direct relationships between sexually objectifying media and the internalization of beauty ideals, self‐objectification, and body surveillance differed across the types of sexually objectifying media. The discussion focuses on the implications of these findings to explain self‐objectification among girls. 相似文献
107.
Laura E. Turner Henrik Kragh Sørensen 《Centaurus; international magazine of the history of science and medicine》2013,55(4):385-411
As a reaction to the changed political landscape in Scandinavia following the dissolution of the union between Norway and Sweden in 1905, the prominent Swedish mathematician Gösta Mittag‐Leffler extended ‘a brotherly hand,’ calling for Scandinavian colleagues to meet for a congress of mathematicians in Stockholm in 1909. This event became the first in a series of biannual meetings which proved to be an important institution for Scandinavian mathematics. During the first decades after 1909, the congresses would form and consolidate themselves through the construction of a new Scandinavian identity for mathematicians which developed alongside and in relation to both international and national contexts and developments. In this paper, we shall demonstrate that these meetings served a complex set of agendas at the individual, national, and international level. In particular, they reflect a changing conception of cooperation in science for mutual cultural gain combined with a flexible institutionalisation that allowed the Scandinavian mathematicians to use the congresses for various diplomatic ambitions. We base our analyses of the Scandinavian Congresses of Mathematics on the notion of a shared ‘conational’ identity developed adjacent to national identities. We then analyse the formation, consolidation, delineation, and reflections of this institution in order to demonstrate how the efforts to unite Scandinavian mathematicians were contingent on and influenced by simultaneous currents of internationalisation and shared history, culture, and language in the Scandinavian region. 相似文献
108.
Merichel Plaza Susana Santoyo Laura Jaime Belkis Avalo Alejandro Cifuentes Guillermo Reglero Guillermo García-Blairsy Reina Francisco Javier Señoráns Elena Ibáñez 《LWT》2012,46(1):245-253
Chlorella vulgaris has been referred as a potential source of bioactive compounds (carotenoids and fatty acids). In this work, the ability of an environmentally friendly extraction technique such as Pressurized Liquid Extraction (PLE) and a traditional extraction technique such as Ultrasound-Assisted Extraction (UAE) to obtain functional compounds from C. vulgaris, at analytical scale, has been demonstrated. Seeked bioactivities were antioxidant and antimicrobial, for their interest in the food industry. Therefore, a methodology including analytical extraction, in-vitro assays and chemical characterization via HPLC-DAD and GC–MS has been used to determine the interest of Chlorella as a source of functional food ingredients. Results demonstrated that PLE provide higher yields than UAE while similar bioactivities were obtained. Important concentration of carotenoids (lutein, neoxanthin, β-carotene, etc.), chlorophylls, sterols, phytols, and fatty acids (among others) have been found in Chlorella extracts that could be correlated to the observed biological activity. 相似文献
109.
Kanchala Sudtachat Maria E. Mayorga Laura A. McLay 《International Transactions in Operational Research》2014,21(4):581-617
Emergency medical service (EMS) systems provide medical care and transportation. While many real‐world systems use multiple vehicle types to attend different call priorities, few guidelines exist about which vehicles to allocate in multitiered responses where more than one vehicle is sent per call. This paper makes recommendations for multiple‐unit dispatch to multiple call priorities based on simulation optimization and heuristics. The objective is to maximize the overall expected survival probability of patients classified as “life‐threatening”. We assume two types of medical units and three call priorities; and that information may be updated when the medical unit arrives on‐scene. First, we study the optimal dispatching policies through several examples. Numerical results show that dispatching while considering call priorities, rather than dispatching the closest units, improves EMS system effectiveness. A heuristic algorithm is developed for large‐scale problems. A comparison between the heuristic and closest policy is demonstrated using real‐world data. 相似文献
110.
Nick Gravish Mateo Garcia Nicole Mazouchova Laura Levy Paul B. Umbanhowar Michael A. D. Goodisman Daniel I. Goldman 《Journal of the Royal Society Interface》2012,9(77):3312-3322
Social insects work together to complete tasks. However, different individuals within a colony may vary in task proficiency. We investigated if fire ant (Solenopsis invicta) worker body size influenced the ability to construct tunnels—a key component of subterranean nests. We monitored excavation by worker groups in a substrate of small wetted glass particles in quasi-two-dimensional arenas. Morphological and network features of the tunnel system were measured. Total tunnel area did not differ significantly between groups of large and small workers, although the tunnel area of control sized workers was significantly larger than that of large workers. Moreover, large workers created wider but shorter tunnels, with slower growth rate of tunnel number. However, edge–vertex scaling and degree distribution of the tunnel network were similar across all treatments. In all cases, the amount of excavated material was correlated with the number of active workers. Our study reveals that morphological features of excavated tunnels show modest variation when constructed by workers of varying sizes, but topological features associated with the tunnel network are conserved. These results suggest that important behavioural aspects of tunnel construction—and thus nest building—are similar among morphologically distinct members of fire ant societies. 相似文献