Mercury contamination in fish from gold mining areas in Indonesia and human health risk assessment

This study investigates the effects on fish and assesses human health hazards from mercury released in two gold mining areas in Indonesia: Tatelu (North Sulawesi Province) and Galangan (Katingan District, Central Kalimatan Province). In Tatelu, 154 fish specimens of 10 freshwater species were collected, as well as five marine species from the fish market. The mean concentration of total mercury in muscles of freshwater fish from this area was 0.58+/-0.44 microg/g, with more than 45% of fish having Hg levels above the WHO guideline for human consumption of 0.5 microg/g. In Galangan, where 263 fish specimens of 25 species were collected, the total mercury in muscles averaged 0.25+/-0.69 microg/g. Excluding data from flooded open pits in sub-area P4, mean Hg levels in fish from Galangan were 2 to 4 times lower than 0.5 microg/g, while fewer than 10% of fish from Galangan exceeded WHO guidelines. The Hazard Quotient (HQ) was applied to both areas to determine the threat of MeHg exposure for communities in both areas. The HQ is a risk assessment indicator which defines the ratio of exposure level to a single substance in relation to a reference dose. Samples from Tatelu (excluding marine species) had an HQ above one, while those from Galangan resulted in values of 2.4 for the whole area and 9.9 for sub-area P4, pointing to potentially harmful fish consumption for the local population. By using the single-compartment model to estimate mercury levels in blood and hair from daily intake dose, sub-area P4 showed the highest levels, higher than the upper limit guideline for pregnant women, but still lower than threshold levels associated with observed clinical effects.     

Detection of enteric viruses, Giardia and Cryptosporidium in two different types of drinking water treatment facilities

In this study, two types of drinking water treatment facilities (two conventional drinking water treatment plants (DWTPs) and two compact units (Cus)) were compared referring to their production capacity. Water samples were collected from three main points: (a) different water treatment steps (b) washings of sand filters and (c) distribution system at different distances from the water treatment plants. Both viruses and protozoa were concentrated from each water sample by adsorption and accumulation on the same nitrocellulose membrane filters (0.45 microm pore size). Enteroviruses were detected by plaque infectivity assay in BGM cells and HAV, HEV and Norovirus were detected by RT-PCR. Giardia and Cryptosporidium were detected by conventional staining methods and PCR. The results revealed that enterovirus load at the intake ranged between 10-15 PFU/L for the two compact units and between 4.5 and 75 PFU/L for the two conventional DWTPs. The virus load in distribution system of the first type DWTPs at 1 km from the plant was the same as that of the intake. Viruses in the other type of treatment plants CUs at 1, 5 and 7 km, were much reduced. Investigation of raw water sediments of the two DWTPs showed enterovirus counts between 12 and 17.5 PFU/L. Virus count was reduced in sand of filters after washing. Giardia cysts were equally detected by microscopy and PCR in only intake samples of EL-Hawamdia CU (33.3%) and Meet Fares DWTP (50%). Cryptosporidium oocysts were equally detected by microscopy and PCR in intake samples of Abo EL-Nomros CU (100%), EL-Hawamdia CU (66.7%) and Fowa DWTP (50%). At Meet Fares DWTP three positive intake samples for Cryptosporidium were detected by PCR, compared with only two positive samples by microscopy. Giardia cysts and Cryptosporidium oocysts were detected in raw water sediment and sand of filters before washing. Only one sample from Meet Fares DWTP sand of filters after washing was positive for both Giardia and Cryptosporidium. It can be concluded that the poor microbial quality of the water may be due to improper operational skills and management of the various water treatment plants (especially at the two high capacity treatment plants).     

Assessment of wind load factors for hurricane-prone regions

We study the issue of whether the wind load factors specified in the ASCE 7–95 Standard for hurricane- prone regions on the one hand and extratropical storm regions on the other are mutually consistent with respect to risk. We consider structures or elements whose design is governed by wind loads and for which wind directionality effects are not significant. We present estimates according to which ASCE 7–95 Standard provisions for wind loads inducing the design strength result in (1) safety levels that are considerably lower for hurricane-prone than for extratropical storm regions, and (2) estimates of mean recurrence intervals of hurricane wind loads inducing the design strength of about 500 y if epistemic uncertainties are neglected, and significantly lower than 500 years otherwise.     

Permeability changes in granite with crack growth during immersion in hot water

Permeability tests and observation of induced, as well as inherent, cracks have been carried out to quantitatively explain permeability changes in granite taking place during a weathering test in terms of micro-structure by cracks. To this end, permeability tensors were estimated from observations of crack systems using the Replica Method and of the crack apertures with a scanning electron microscope. The following conclusions were reached: the drastic increase in permeability around an immersion of 1000 d in 90°C water could not be explained by the existence of a percolation threshold because all the specimens were percolative in the sense that the traces of crack tensor were above the critical density for percolation. On the other hand, SEM observation revealed that, with immersion, crack apertures widened and their distribution spread. Permeability estimated using the third (not the first) moment of observed apertures showed the increase in measured permeability well. When evaluating the connectivity parameter λ to give a quantitative accordance between measured and estimated permeability, it had a constant value as low as 0.17, irrespective of the immersion period, in spite of the higher density of micro-cracks than the percolation threshold. It can be said that, in considering the natural three-dimensional crack system, the third moment of physical apertures plays a more important role in predicting the permeability and λ is greatly affected by the spread of crack apertures.     

Separation and determination of mineral, animal and vegetable oils in water with the use of molecular sieve 5A

Mineral, animal and vegetable oils in water were separated and determined by the infrared method with the use of molecular sieve 5A (MS 5A). After being extracted by carbon tetrachloride, the extract were treated with MS 5A. Mineral oil was not adsorbed on MS 5A, but animal and vegetable oils were. Oils extracted with carbon tetrachloride were measured by the peak at 2950 cm⁻¹ due to the vibration of the CH groups.     

Radiocesium in muscle tissue of reindeer and pike from northern Sweden before and after the Chernobyl accident. A retrospective study on tissue samples from the Swedish Environmental Specimen Bank.

After the Chernobyl accident in April 1986, considerable deposition of radionuclides occurred regionally in eastern, central and northwestern Sweden. Locally, the fallout of radiocesium exceeded the remainder from atmospheric nuclear weapons tests by several magnitudes. Since the end of the 1960s samples of organs from various plant and animal species, annually collected at different localities, have been preserved in the Swedish Environmental Specimen Bank (ESB). In this work samples from the ESB have been used for retrospective studies of radioactive pollution. The activities of Cs-134 and Cs-137 in muscle tissues from reindeer, Rangifer tarandus, and pike, Esox lucius, preserved in the ESB, were measured. The samples were collected annually; the reindeer at three localities in northern Sweden and the pike at one of them. In material collected prior to the Chernobyl accident, the levels of Cs-137 were 57-180 Bq/kg in reindeer and 14-24 Bq/kg in pike, fresh weight basis. These levels relate to earlier nuclear bomb tests. A significant decrease was found in pike during the pre-Chernobyl period (1971-86). In post-Chernobyl samples the burden of Cs-137 varied from amounts equal to the former levels in the northernmost locality and up to 80 times higher for the maximum values in the southernmost locality. The highest value recorded was 18,425 Bq/kg in reindeer. The geographic variations in reindeer from Chernobyl fallout were in accordance with the pattern of deposition estimated by aircraft surveys performed in May 1986. The ratio between 'new' and 'old' radiocesium burdens in pike, caught in 1987, approached the corresponding ratio for reindeer grazing in the precipitation area of the lake; 33 and 19, respectively.     

Contribution à l'étude de la détermination des conditions de formation des haloformes

In recent years the presence of haloforms has been observed by some researchers in waters of various origins.The aim of this study is to determine the conditions in which chloroform is formed by chlorination of aqueous solutions of some organic compounds.The first part of this work was carried out by chlorination of synthetic basic solutions of acetone and thus enabled us to determine the reagent concentrations needed to obtain the maximum quantity of chloroform.We studied next the effect of chlorination on organic material solutions (humic acids and phenols), in the course of an oxidation treatment; the results obtained by chlorination of aqueous solutions of humic substances show that the quantity of chloroform produced passes through a maximum value which depends on the time of ozonisation. Similar results were obtained for the chlorination of phenol solutions during oxidation by the u.v. + H₂O₂ system. These results may be explained by the partial degradation of the molecules which leads in the formation of reactional intermediate precursors of the haloform reaction. We undertook at the same time the study of a rapid test allowing us to detect the presence of haloform reaction precursors in a certain sample of water.     

Oxygen transfer in a rotating disc treatment plant

In this paper experiments are described in a bio-disc pilot plant. A new method has been developed for a reproducible measurement of the thickness of the waterfilm on the rotating discs. The results were used to calculate the oxygen transfer coefficient into water (K_L). The values of K_L, calculated in this way, appeared to be considerably higher than experimental K_L-values, which is mainly due to the fact that the water layer on the rotating disc is outdistanced by the disc. This effect has not been taken into account in the literature so far.