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91.
The Sludge Treatment Reed Bed (STRB) technology is a cost-efficient and environmentally friendly technology to dewater and mineralize surplus sludge from conventional wastewater treatment systems. Primary and secondary liquid sludge is loaded onto the surface of the bed over several years, where it is dewatered, mineralized and turned into a biosolid with a high dry matter content for use as an organic fertilizer on agricultural land. We analysed the concentrations of five organic micropollutants (galaxolide, tonalide, cashmeran, celestolide and DEHP) and six heavy metals (Pb, Ni, Cu, Cd, Zn and Cr) in the accumulated sludge in a 20-year old STRB in Denmark in order to assess the degradation and fate of these contaminants in a STRB and the relation to sludge composition. The results showed that the deposited sludge was dewatered to reach a dry matter content of 29%, and that up to a third of the organic content of the sludge was mineralized. The concentrations of heavy metals generally increased with depth in the vertical sludge profile due to the dewatering and mineralization of organic matter, but in all cases the concentrations were below the European Union legal limits for agricultural land disposal. The concentrations of fragrances and DEHP ranged from 10 to 9000 ng g−1 dry mass. The attenuation of hydrophobic micropollutants from the top to the bottom layer of the reed bed ranged from 40 to 98%, except for tonalide which increased significantly with sludge depth, and consequently showed an unusual depth distribution of the galaxolide/tonalide ratio. This unexpected pattern may reflect changes imposed by a long storage time and/or different composition of the fresh sludge in the past. The lack of a significant decreasing DEHP concentration with sludge age might indicate that this compound is very persistent in STRBs. In conclusion the STRB was a feasible technology for sludge treatment before its land disposal. 相似文献
92.
Flocculation of activated sludge flocs by stimulation of the aerobic biological activity 总被引:4,自引:0,他引:4
Activated sludge flocs are known to deflocculate under short-term anaerobic conditions, but little is known about possible reflocculation under subsequent aerobic conditions. When activated sludge flocs from two wastewater treatment plants deflocculated under anaerobic conditions with well-defined shear conditions, they could be almost, but not completely, reflocculated by aeration for 1-2 h under the same shear conditions. If the biological activity was reduced by adding azide, chloramphenicol or by decreasing the temperature, no or only very little reflocculation took place. This indicated that the reflocculation was under direct or indirect microbial control. Only a small part of the reflocculation was due to improved flocculation properties obtained by oxidation of Fe(II) to Fe(III), which is a better flocculant. Fe(II) was produced under the anaerobic conditions by microbial iron reduction, and it was oxidized to Fe(III) within less than one hour after the aeration was started. However, by comparing two different sludges with different capabilities for iron reduction, iron oxidation and responses to substrate addition, it was found that the aerobic biological activity most likely was of greatest significance for the observed reflocculation and floc formation under aerobic conditions. This was further supported by adding organic substrates (glucose or ethanol) during the aerobic reflocculation phase, which promoted reflocculation. However, some substrates had the opposite effect (acetate and lactate), where a deterioration of the reflocculation was observed, probably due to different responses from different groups of microorganisms in the sludges. 相似文献
93.
Kale, lettuce, carrots and potatoes were grown in 20 experimental plots surrounding a wood preservation factory, to investigate the amount and pathways for plant uptake of arsenic and chromium. Arsenate used in the wood preservation process is converted to the more toxic arsenite by incineration of waste wood and is emitted into the atmosphere. Elevated concentrations of inorganic arsenic and chromium were found both in the test plants and in the soil around the factory. Multivariate statistical analysis of the results indicated that the dominating pathway of arsenic and chromium from the factory to the leafy vegetables grown nearby was by direct atmospheric deposition, while arsenic in the root crops originated from both the soil and the atmosphere. Consumption of vegetables grown near the source would result in an increased intake of inorganic arsenic, but the intake via the total diet was estimated to be below the provisional tolerable daily intake for inorganic arsenic established by FAO/WHO. 相似文献
94.
Ana B. Lanham Adrian Oehmen Aaron M. Saunders Gilda Carvalho Per H. Nielsen Maria A.M. Reis 《Water research》2013
This study analysed the enhanced biological phosphorus removal (EBPR) microbial community and metabolic performance of five full-scale EBPR systems by using fluorescence in situ hybridisation combined with off-line batch tests fed with acetate under anaerobic–aerobic conditions. The phosphorus accumulating organisms (PAOs) in all systems were stable and showed little variability between each plant, while glycogen accumulating organisms (GAOs) were present in two of the plants. The metabolic activity of each sludge showed the frequent involvement of the anaerobic tricarboxylic acid cycle (TCA) in PAO metabolism for the anaerobic generation of reducing equivalents, in addition to the more frequently reported glycolysis pathway. Metabolic variability in the use of the two pathways was also observed, between different systems and in the same system over time. The metabolic dynamics was linked to the availability of glycogen, where a higher utilisation of the glycolysis pathway was observed in the two systems employing side-stream hydrolysis, and the TCA cycle was more active in the A2O systems. Full-scale plants that showed higher glycolysis activity also exhibited superior P removal performance, suggesting that promotion of the glycolysis pathway over the TCA cycle could be beneficial towards the optimisation of EBPR systems. 相似文献
95.
Vollertsen J Nielsen AH Jensen HS Wium-Andersen T Hvitved-Jacobsen T 《The Science of the total environment》2008,404(1):162-170
The widespread occurrence of polybrominated diphenyl ethers (PBDEs) in the environment has attracted considerable attention, leading to concerns about the extent and magnitude of wildlife and human exposure. In this work, we focus on the occurrence and fate of PBDEs in a Norwegian air-plant-herbivore-carnivore system. Specifically, we have analysed for PBDEs in moss, livers from various terrestrial herbivores (moose, grouse, and European roe deer) and, for the first time, livers from the top predator lynx. The samples were collected from different sites and time periods (1990-2004) to identify possible spatial and temporal trends in contaminant levels and patterns. The general finding was that PBDEs were found in all (biotic) samples, although at lower concentrations than previously observed in mammals from the marine environment. The PBDE levels in the herbivores ranged from less than 0.5 ng/g lipid weight to 9.4 ng/g lipid weight as the highest. The median PBDE concentration in lynx was approximately one order of magnitude higher than in the herbivores. In the lynx samples there was a predominance of BDE-153 whereas BDE-47 and 99 dominated in the herbivores. This probably reflects different bioaccumulation properties or metabolic transformation processes of the BDE-congeners, and food choice. Levels of PBDEs in both moss and herbivores showed a general decline towards the northern parts of Norway. No clear temporal trends were observed. The PBDE levels observed in this study were low and are probably of limited toxicological significance. 相似文献
96.
Nielsen Guillaume Janin Amelie Coudert Lucie Blais Jean Francois Mercier Guy 《Mine Water and the Environment》2018,37(1):42-55
Mine Water and the Environment - Passive treatment is a promising, green technology that is increasingly being used for mine drainage treatment. However, several challenges remain concerning its... 相似文献
97.
Mette B. Petersen Jeanette Pedersen Dinah L. Holm Matthew Denwood Liza R. Nielsen 《Journal of dairy science》2018,101(8):7383-7396
Mycoplasma bovis is an important pathogen causing disease and substantial economic losses in cattle. However, knowledge of the dynamics of antibody responses in individual cows in the face of an outbreak is currently extremely limited. The use of commercial antibody tests to support clinical decision-making and for surveillance purposes is therefore challenging. Our objective was to describe the dynamics of M. bovis antibody responses in 4 Danish dairy herds experiencing an acute outbreak of M. bovis-associated disease, and to compare the antibody dynamics between dairy cows with different disease manifestations. A total of 120 cows were examined using a standardized clinical protocol and categorized into 4 disease groups: “mastitis,” “systemic,” “nonspecific,” and “none.” Paired blood and milk samples were collected and tested using a commercial M. bovis antibody–detecting ELISA. Plots of raw data and generalized additive mixed models with cow and herd as random effects were used to describe serum and milk antibody dynamics relative to the estimated time of onset of clinical disease. Cows with mastitis had high optical density measurement (ODC%) of antibodies in both milk and serum at disease onset. The estimated mean ODC% in milk was below the manufacturer's cut-off for the other groups for the entire study period. The estimated mean serum ODC% in the “systemic” group was high at onset of disease and stayed above the cut-off until 65 d after disease onset. However, the lower 95% confidence interval (CI) for the mean ODC% was only above the manufacturer's cut-off between 7 and 17 d after onset of disease. The CI of the “systemic” and “none” groups did not overlap at any time between the day of disease onset and 65 d after disease onset, and the estimated mean ODC% for both the “nonspecific” and “none” groups were generally below the cut-off for the majority of the study period. In conclusion, the serum antibody responses were highly dynamic and showed a high level of variation between individual cows. This strongly suggests that serology is unlikely to be useful for individual diagnosis of M. bovis-associated disease in dairy cows. However, it might still be useful for herd- or group-level diagnosis. Antibodies in milk were only increased in cows with M. bovis mastitis, indicating that milk antibody measurements only have diagnostic utility for cows with mastitis. 相似文献
98.
P.M. Nielsen 《Food Biotechnology》2013,27(3):119-156
The enzyme transglutaminase is able to modify proteins by catalyzing crosslinking reactions, deamidation and incorporation of amines. These possibilities of changing the structure of proteins have been the subject of an increasing number of papers during the last 10 years. This work has shown that the reaction is dependent on the source of transglutaminase as well as substrate and conformation of the substrate. Also parameters such as pH, temperature, concentration of substrate and enzyme, salt etc. have been discussed. This paper is intended to be a review of the scientific as well as the patent literature in order to elucidate where in the future we most likely will find transglutaminase utilized in the food industry and in technical applications. 相似文献
99.
Hansen K.L. Udesen J. Thomsen C. Jensen J.A. Nielsen M.B. 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2009,56(1):91-100
Conventional Doppler methods for blood velocity estimation only estimate the velocity component along the ultrasound beam direction. This implies that a Doppler angle under examination close to 90° results in unreliable information about the true blood direction and blood velocity. The novel method transverse oscillation (TO), which combines estimates of the axial and the transverse velocity components in the scan plane, makes it possible to estimate the vector velocity of the blood regardless of the Doppler angle. The present study evaluates the TO method with magnetic resonance phase contrast angiography (MRA) by comparing in vivo measurements of stroke volume. Eleven healthy volunteers were included in this prospective study. From the obtained data sets recorded with the 2 modalities, vector velocity sequences were constructed and stroke volume calculated. Angle of insonation was approximately 90° for TO measurements. The correlation between the stroke volume estimated by TO and MRA was 0.91 (p < 0.01) with the equation for the line of regression: MRA = 1.1?TO-0.4. A Bland-Altman plot was additionally constructed where the mean difference was 0.2 ml with limits of agreement at ?1.4 ml and 1.9 ml. The results indicate that reliable vector velocity estimates can be obtained in vivo using the presented angle-independent 2-D vector velocity method. The TO method can be a useful alternative to conventional Doppler systems by avoiding the angle artifact, thus giving quantitative velocity information. 相似文献
100.