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61.
ABSTRACTNonlinear, large inertia with long dead time is always associated with the main steam temperature parameter in coal fired power plant. Successful control of the main steam temperature within ±2°C of its setpoint is the ultimate target for coal-fired power plant operators. Two of the most common main steam temperature circuit are primary superheater spray and secondary superheater spray. Various methods were used to model the primary superheater spray control valve opening, and the neural network remains one of the most popular choices among researchers. It remains inconclusive which neural network algorithm types, setup, number of layers, and training algorithm will give the best result. As such, the paper shows the best setup for the neural network algorithm based on sensitivity analysis methodology for one hidden layer. The inputs selected for the neural network are generator output, main steam flow, total spray flow, and secondary superheater outlet steam temperature, while the output selected is primary spray flow control valve opening. 相似文献
62.
Loredana-Mirela Lupu Pascal Wiegand Daria Holdschick Delia Mihoc Stefan Maeser Stephan Rawer Friedemann Vlklein Ebrahim Malek Frederik Barka Sascha Knauer Christina Uth Julia Hennermann Wolfgang Kleinekofort Andreas Hahn Günes Barka Michael Przybylski 《International journal of molecular sciences》2021,22(23)
Analytical methods for molecular characterization of diagnostic or therapeutic targets have recently gained high interest. This review summarizes the combination of mass spectrometry and surface plasmon resonance (SPR) biosensor analysis for identification and affinity determination of protein interactions with antibodies and DNA-aptamers. The binding constant (KD) of a protein–antibody complex is first determined by immobilizing an antibody or DNA-aptamer on an SPR chip. A proteolytic peptide mixture is then applied to the chip, and following removal of unbound material by washing, the epitope(s) peptide(s) are eluted and identified by MALDI-MS. The SPR-MS combination was applied to a wide range of affinity pairs. Distinct epitope peptides were identified for the cardiac biomarker myoglobin (MG) both from monoclonal and polyclonal antibodies, and binding constants determined for equine and human MG provided molecular assessment of cross immunoreactivities. Mass spectrometric epitope identifications were obtained for linear, as well as for assembled (“conformational”) antibody epitopes, e.g., for the polypeptide chemokine Interleukin-8. Immobilization using protein G substantially improved surface fixation and antibody stabilities for epitope identification and affinity determination. Moreover, epitopes were successfully determined for polyclonal antibodies from biological material, such as from patient antisera upon enzyme replacement therapy of lysosomal diseases. The SPR-MS combination was also successfully applied to identify linear and assembled epitopes for DNA–aptamer interaction complexes of the tumor diagnostic protein C-Met. In summary, the SPR-MS combination has been established as a powerful molecular tool for identification of protein interaction epitopes. 相似文献
63.
This study includes design and experimental analysis of a solar domestic water heating system. Water heating systems with glazed and unglazed collectors were constructed and tested at Dhaka, Bangladesh, at a latitude of 23.7 °N. Collector thermal efficiency and capability of raising water temperature were considered as performance evaluation measures. A typical day analysis showed that collector efficiency varied with time and touched its peak at around 12:00 h. During testing, the efficiency of the glazed collector increased by about 70.3% when compared with the unglazed collector. Average collector efficiency over the whole test period was also estimated to be 57.3% and 33.7% for glazed and unglazed collectors, respectively. Maximum water temperatures measured at daytime user outlets were, respectively, 82.4 °C and 65.5 °C for domestic water heating systems with glazed and unglazed collectors and approximated to be 49 °C and 32 °C higher than the ambient temperature. The glazed collector eventually offered significantly higher performances over the unglazed collector in improving system performance. 相似文献
64.
A reactive cyclodextrin was synthesised by reacting β‐cyclodextrin with itaconic acid to enable it to fix permanently onto cellulosic materials. Because synthesis is a complicated process that is greatly influenced by many factors, the response surface methodology was applied in this study to optimise production. To investigate the efficiency of the esterification reaction, the amount of carboxyl groups and the double bond content of the end product were measured and employed as the responses. The 3D response surface plots and the contour plots derived from the mathematical models were applied to evaluate the interactive effects of parameters affecting the reaction, such as itaconic acid and catalyst concentrations, material to liquor ratio, temperature and time of reaction. The amount of carboxyl groups and the double bond content of cyclodextrin itaconate (about 175 and 150 meq./100 g CDI, respectively) in the optimum conditions indicated that one to two itaconic acid molecules could react with cyclodextrin according to the esterification reaction. In addition, the presence of the new supplementary groups on cyclodextrin could effect on the aggregation behaviour of this new cyclodextrin derivative as demonstrated by dynamic light scattering and AFM. 相似文献
65.
Listeria monocytogenes was isolated from raw milk, whey and curdled milk produced and collected in the region of Algiers and Blida between September 2003 and July 2004. Four out of 153 (2.61%) farm milk samples and 6 out of 80 (7.50%) tankers' samples tested positive for L. monocytogenes. All samples of whey and curdled milk (n=12) tested negative for L. monocytogenes, but 2 of 22 (9%) samples of whey were contaminated by L. innocua. L. monocytogenes isolates were grouped by a multiplex PCR assay; all isolates belonged to the PCR-group IVb, which corresponds to serovars 4b, 4d and 4e. L. monocytogenes isolates were characterized by Pulsed-Field Gel Electrophoresis (PFGE). The combination of AscI and ApaI macrorestriction patterns yielded five different pulsovars (I to V). The results indicate that raw milk, and raw milk products are potential sources of the L. monocytogenes and represent a potential risk for consumers. 相似文献
66.
dos Reis CB Barreiro JR Moreno JF Porcionato MA Santos MV 《Journal of dairy science》2011,94(9):4406-4412
The objectives of this study were (1) to determine the sensitivity (Se) and specificity (Sp) of somatic cell count (SCC) thresholds to identify subclinical mastitis in Gyr cows caused by major and minor pathogens; (2) to study the effects of month of sampling, rear or front mammary quarters, herd, intramammary infection (IMI), and bacterial species on SCC at quarter level; and (3) to describe the prevalence of IMI in Gyr cows in commercial dairy herds. In total, 221 lactating Gyr cows from 3 commercial dairy farms were selected. Milk samples were collected from individual quarters once a month for 1 yr from all lactating cows for SCC and bacteriological analysis. Mammary quarters were considered the experimental units and the SCC results were log10-transformed. Four SCC thresholds (100, 200, 300 and 400 × 103 cells/mL) were used to determine Se and Sp to identify infected mammary quarters. The overall prevalence of IMI in quarter milk samples of Gyr cows was 49.8%, and the prevalence of minor pathogens was higher (31.9%) than that of major pathogens (17.8%). Quarter samples with microbial isolation presented higher SCC compared with negative samples. Sensitivity and Sp of selected SCC thresholds varied according to the group of pathogen (major and minor) involved in the IMI definition. Sensitivity increased and Sp decreased when mammary quarters with only major pathogens isolation were considered positive. The use of a single SCC analysis to classify quarters as uninfected or infected in Gyr cows may not be a useful test for this breed because Se and Sp of SCC at the studied thresholds were low. The occurrence of IMI and the bacterial species are the main factors responsible for SCC variation in mammary quarters of Gyr cows. Milk samples with major pathogens isolation elicited higher SCC than those with minor pathogens. 相似文献
67.
Stéphane Malek 《Journal of Dynamical and Control Systems》2009,15(2):277-305
We construct formal power series solutions of nonlinear partial integro-differential equations with Fuchsian and irregular
singularities at the origin of for given initial conditions being formal power series. We give sufficient conditions under which there exist actual sectorial
holomorphic solutions which are Gevrey asymptotic to the given formal series solutions for given 1-summable formal series
initial conditions. A phenomenon of small divisors is observed for the appearance of singularities of the Borel transform
of the constructed formal series due to the presence of the Fuchsian singularity. This property has an effect on the Gevrey
asymptotic order for the constructed holomorphic solutions which becomes larger than the Gevrey order of the initial conditions.
相似文献
68.
Olivier Malek Jef Vleugels Yeczain Perez Patrick De Baets Junhu Liu Sven Van den Berghe Bert Lauwers 《Materials Chemistry and Physics》2010
The interrelationships between the dispersion of the ZrO2 phase and the electrical discharge machining (EDM) behaviour of WC based composites with 0, 5 or 10 vol% of ZrO2 are investigated. Special attention was given to the homogeneity of the ultrafine WC – nanometric ZrO2 powder based composites which were fully densified by means of pulsed electric current sintering (PECS). X-ray photoelectron spectroscopy (XPS) measurements revealed the presence of a nanometric WO3 layer on the EDM surface, confirming oxidation as the major material removal mechanism (MRM). The surface roughness after the final EDM finishing regime was strongly influenced by the composite homogeneity and could be reduced down to 0.15 μm (Ra) on agglomerate-free composites. Residual stress measurements indicated that EDM did not introduce a significant amount of surface stresses, especially not after the final finishing regime. XRD measurements of EDM surfaces however indicated surface depletion of ZrO2 by the formation of ZrC and W2C during spark erosion. 相似文献
69.
The synthesized zeolite NaY from rice husk ash (RHA) and the commercial zeolite NaY both modified with surfactants in amounts equal to 50%, 100% and 200% of their external cation exchange capacity (ECEC) were used to remove chromate and arsenate anions from aqueous solutions. While the unmodified zeolite Y had little or no affinity for the Cr(VI) and As(V) anionic species, the surfactant-modified zeolite Y (SMZY) showed significant ability to remove of these anions from the aqueous solutions. The highest chromates and arsenates adsorption efficiency was observed from solutions of pH values 3 and 8, respectively because of the dominance of the univalent species of both anions. The adsorption equilibrium data were best fitted with the Langmuir isotherm model with the highest removal capacities observed for the SMZY initially prepared considering the hexadecyltrimethyl ammonium (HDTMA) amount equal to the 100% of the ECEC of zeolite Y. Synthesized SMZY remove Cr(VI) and As(V) more than the corresponding commercial one due to its lower silica to alumina ratio. Thus, the HDTMA-covered modified zeolite Y synthesized using RHA can be used to remove Cr(VI) and As(V) from water. 相似文献
70.
Ryan Fitzgerald Andrew Olsen Jessica Nguyen Winifred Wong Malek El Muayed Joshua Edwards 《International journal of molecular sciences》2021,22(1)
Cadmium (Cd) is an anthropogenic as well as a naturally occurring toxicant associated with prediabetes and T2DM in humans and experimental models of Cd exposure. However, relatively few studies have examined the mechanism(s) of Cd-induced hyperglycemia. The purpose of this study was to examine the role of pancreatic islets in Cd-induced hyperglycemia. Male Sprague–Dawley rats were given daily subcutaneous doses of Cd at 0.6 mg/kg over 12 weeks. There was a resulting time-dependent increase in fasting blood glucose and altered insulin release in vitro. Islets isolated from control (saline-treated) and Cd-treated animals were incubated in low (0.5 mg/mL) or high (3 mg/mL) glucose conditions. Islets from 12 week Cd-treated animals had significantly less glucose-stimulated insulin release compared to islets from saline-treated control animals. The actual Cd content of isolated islets was 5 fold higher than the whole pancreas (endocrine + exocrine) and roughly 70% of that present in the renal cortex. Interestingly, islets isolated from Cd-treated animals and incubated in high glucose conditions contained significantly less Cd and zinc than those incubated in low glucose. These results show that within whole pancreatic tissue, Cd selectively accumulates in pancreatic islets and causes altered islet function that likely contributes to dysglycemia. 相似文献