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排序方式: 共有897条查询结果,搜索用时 390 毫秒
101.
Dr. Umberto Maria Battisti Dr. Chunxia Gao Oscar Nilsson Dr. Fady Akladios Dr. Aleksei Lulla Dr. Agnieszka Bogucka Dr. Amalyn Nain-Perez Dr. Liliana Håversen Dr. Woonghee Kim Prof. Jan Boren Prof. Marko Hyvönen Prof. Mathias Uhlen Prof. Adil Mardinoglu Prof. Morten Grøtli 《Chembiochem : a European journal of chemical biology》2023,24(1):e202200339
Enzymes are effective biological catalysts that accelerate almost all metabolic reactions in living organisms. Synthetic modulators of enzymes are useful tools for the study of enzymatic reactions and can provide starting points for the design of new drugs. Here, we report on the discovery of a class of biologically active compounds that covalently modifies lysine residues in human liver pyruvate kinase (PKL), leading to allosteric activation of the enzyme (EC50=0.29 μM). Surprisingly, the allosteric activation control point resides on the lysine residue K282 present in the catalytic site of PKL. These findings were confirmed by structural data, MS/MS experiments, and molecular modelling studies. Altogether, our study provides a molecular basis for the activation mechanism and establishes a framework for further development of human liver pyruvate kinase covalent activators. 相似文献
102.
Marko R. Djokic Kevin M. Van Geem Carlo Cavallotti Alessio Frassoldati Eliseo Ranzi Guy B. Marin 《Combustion and Flame》2014
The importance of 1,3-cyclopentadiene (CPD) and cyclopentadienyl (CPDyl) moieties in the growth of polycyclic aromatic hydrocarbons (PAHs) was studied using new experimental data and ab initio calculations. The experimental investigation was performed in a tubular continuous flow pyrolysis reactor under both high (24molN2/molCPD) and low (5molN2/molCPD) nitrogen dilutions, covering a temperature range of 873–1123 K, at a fixed pressure of 1.7 bara. At the most severe conditions up to 84% of CPD is converted, and the amount of PAHs is more than 65 wt%. Major products observed during CPD pyrolysis were benzene, indene, methyl-indenes and naphthalene, in line with previous studies. On-line GC × GC-FID/(TOF-MS) also allowed to quantify minor species (methane, toluene, styrene, phenanthrene, anthracene, etc.), never reported before at this level of accuracy. The new experimental data have been used to further analyze the role of the successive interactions of CPD, indene, and naphthalene as well as the recombination and addition reactions of their resonantly stabilized radicals and refine their kinetics. The results of the modeling study are in good agreement with existing and new experimental observations. 相似文献
103.
104.
105.
A mechanical thermostat is a device that switches heating or cooling appliances on or off based on temperature. For this kind of use, electronic or mechanical switching concepts are applied. During the production of electrical contacts, several irregularities can occur leading to improper switching events of the thermostat electrical contacts. This paper presents a non-obstructive method based on the fact that when the switching event occurs it can be heard and felt by human senses. We performed several laboratory tests with two different methods. The first method includes thermostat switch sound signal analysis during the switching event. The second method is based on sampling of the accelerometer signal during the switching event. The results show that the sound analysis approach has great potential. The approach enables an accurate determination of the switching event even if the sampled signal carries also the switching event of the neighbour thermostat. 相似文献
106.
Istvan Kucora Pera Paunjoric Jasna Tolmac Marko Vulovic James G. Speight 《Petroleum Science and Technology》2017,35(3):213-221
In the petrochemical industry, an industrial pyrolysis furnace is a complicated piece of equipment that functions as both a reactor and high-pressure steam generator. During service, hard deposits of carbon (coke) build up on the inner wall of the tube, reducing heat transfer and restricting the flow of the hydrocarbon feedstocks. In this paper, an overview of coke formation, the impact of coke formation, and the methods by which coke formation can be prevented, as well as methods that can be successful in decoking of pyrolysis furnaces, is presented. 相似文献
107.
Ville Rimpiläinen Sami Poutiainen Lasse M. Heikkinen Tuomo Savolainen Marko Vauhkonen Jarkko Ketolainen 《Chemical engineering science》2011,(18):4090
Electrical capacitance tomography (ECT) was utilized for monitoring of high-shear mixing and high-shear granulation processes. A finite element method (FEM)-based reconstruction algorithm was utilized to take into account the specific geometrical characteristics of the experimental set-up. Two-dimensional ECT tomograms, mixing index curves and permittivity fractions were computed based on the measurements, and their suitability in the analysis of the processes was assessed. It was found that the different mixing processes and the different granulation processes could be analyzed based on these quantities. 相似文献
108.
Influence of cardiac motion on diffusion-weighted magnetic resonance imaging of the liver 总被引:1,自引:1,他引:0
Thomas C. Kwee Taro Takahara Tetsu Niwa Marko K. Ivancevic Gwenael Herigault Marc Van Cauteren Peter R. Luijten 《Magma (New York, N.Y.)》2009,22(5):319-325
Purpose
To assess cardiac motion-induced signal loss in diffusion-weighted magnetic resonance imaging (DWI) of the liver using dynamic DWI. 相似文献109.
Vitoslav Bratuš Franc Kosel Marko Kovač 《Journal of Materials Processing Technology》2010,210(2):396-407
Today engineers involved in the stamping process used for high-volume production of rotor and stator laminations are faced with a great challenge to achieve extremely narrow dimensional and geometrical tolerance on their products. Because materials are produced by different suppliers, adjustments of technological parameters to the emerging differences are required to maintain the high quality of products. An upgraded engineering method was developed in which electrical steel sheet of semi-finish grade was not treated traditionally, but as a 3D body with orthotropic material behaviour. The round profile of the cutting edge was studied from the experimental and numerical point of view. If the completely round punch was used, the profile of the cutting edge appeared as a non-round shape. With additional FEM analyses a new profile of punch was designed as a non-round shape to be able to provide much better circularity. FEM simulation showed that 10 times lower profile deviations were found on the workpiece shaped by the new cutting element profile. 相似文献
110.
Corrosion investigations have been performed on the austenitic structural steel AISI 304L, in comparison with the structural steel AISI 316L, in an aerated and a de-aerated solution, which was leached from low and medium level radioactive waste. On the basis of measured potentio-dynamic anodic polarization curves and the results of cyclic polarization tests, it was found that both types of steel, as well as the corresponding welds, had a high pitting potential and a high protective potential, which means that they have a strong tendency to form a compact and corrosion-resistant passive film. The repassivation capability of both types of steel prevents the occurrence of stress corrosion cracking at the level of concentration of chloride ions which corresponds to the described type of waste, whereas absorbed atomic hydrogen does not reduce toughness or cause hydrogen embrittlement. The results of the research work confirmed that it is possible to use AISI 304L structural steel for the construction of containers for the temporary, 30-year storage of low and medium level radioactive waste. 相似文献