Tanker milk variability according to farm feeding practices: vitamins A and E, carotenoids, color, and terpenoids

The aim of this work was to study the variability in the composition of bulk milk mixtures of fat-soluble compounds (vitamins A and E, carotenoids, and terpenoids) and assess the links with milk production conditions. Milk from 10 collection trips in the French department of the Haute-Loire (10 to 36 herds per trip) was sampled in the tanker twice during the winter period and 3 times during the grazing season. The collection trips differed in their altitude (440 to 1,150 m) and the forage system (grass or based on corn silage). Vitamins A and E, carotenoids, and terpenoids of the 50 tanker loads of milk were analyzed. Data of milk production conditions in the 204 farms made it possible to constitute indicators for the collection trip and to define 50 mean herds. The relationships between mean herd characteristics (breed, stage of lactation, and feed) and milk characteristics were investigated. The constituents of tanker loads of milk were comparable to those observed in milk produced by groups of animals receiving contrasting diets (rich in concentrate or corn silage vs. pasture). The characteristics of the milk differed according to the period; those produced at grazing were more yellow (1.02 ± 0.4; mean of difference) and richer in β-carotene, lutein, vitamin E (2.0 ± 1.2, 0.23 ± 0.12, and 6.1 ± 5.0 μg/g of fat, respectively), and sesquiterpenes (2.7 ± 2.5) than winter. The variations observed for β-carotene, lutein, and vitamin E were linked to the proportion of grazed grass or grass silage in the forage (r = 0.66, 0.69, and 0.51, respectively), unlike the vitamin A content. During grazing, 20 of the 32 terpenoids identified were associated with the proportion of permanent grassland available for grazing or cut. These results show that feeding is an effective way to modify the quality of dairy products, even in the case of bulk tank milk mixtures. Dairy plants could market different milks, which would contain specific compositions.     

A New Validation of Relevant Substances for the Evaluation of Beer Aging Depending on the Employed Boiling System

During the last years changes in aging indicators have been observed, supposedly resulting from modern brewing technology. The Research Brewery Weihenstephan (Forschungsbrauerei Weihenstephan) offers excellent opportunities for comparing different modern wort boiling systems under semi‐industrial conditions. Employing three different boiling systems, nine brews were produced. The resulting worts were compared regarding the most common wort parameters. Furthermore the influence of the different boiling systems on aging indicators in the resulting beers was analyzed using a newly developed mass spectrometry‐based method. The decrease in the total amount of aging indicators in industrial beers over the last years is very likely the result of lower thermal intake in modern brewhouse equipment. The total amount of aging indicators is sufficient to describe the differences in modern boiling systems. In summary, 2‐furfural dominates all other indicators in terms of thermal influence. 2‐Furfuryl ethyl ether can be suggested as good indicator of aging as postulated by Eichhorn, whereas β‐damascenone is questionable as an aging indicator. Supplementary experiments were carried out to investigate the role of the aging indicators as stale flavour components. Because of synergistic effects, many stale flavour compounds act as aroma compounds and not only as indicators.     

The discovery of the nonindigenous,mottled fingernail clam, Eupera cubensis (Prime, 1865) (Bivalvia: Sphaeriidae) in the Chicago Sanitary and Ship Canal (Illinois River Drainage), Cook County,Illinois

The nonindigenous mottled fingernail clam, Eupera cubensis is reported from the Upper Mississippi River Basin for the first time. This record represents a significant northern range expansion for the species in the United States. It appears to be presently confined to a 35-km stretch of the Chicago Sanitary and Ship Canal (CSSC), an artificial waterway that connects the Mississippi River drainage to the Great Lakes. Although the introduction of this species to the Great Lakes basin poses uncertain risks to the general aquatic community, the immediate effects of Eupera cubensis on the fauna of the CSSC are expected to be minimal.     

Increased hydropower production and hydropeaking mitigation along the Upper Inn River (Tyrol,Austria) with a combination of buffer reservoirs,diversion hydropower plants and retention basins

Research on hydropeaking in Austria started in the 1990s and the implementation of the WFD stipulated large research projects since the year 2009. The first research projects supported the process understanding and in a second investigation step, measures were evaluated. The mountains in the region of Tyrol create large heads and thus support the production of flexible energy. In this region, TIWAG is operating 9 large (>10 MW) and 27 small (<10 MW) hydropower plants, with an installed capacity of about 1,550 MW and a mean annual production of 3,000 GWh. As the governmental energy strategy foresees an extension of the hydropower production in Austria, suitable options were selected in 2004 by TIWAG and the water management framework plan for Western Tyrol was developed. This strategic planning instrument proposes five large power plants, with a generation of 1,800 GWh/year of renewable energy, which enables to reach the WFD targets, because the concept includes hydropeaking mitigation by combining buffer reservoirs (impoundments), diversion hydropower plants, and retention basins. We present our promising concept of hydropeaking mitigation and exemplify this based on the GKI, a hydropeaking diversion hydropower plants (HPP) at the Swiss/Austrian border as well as the Silz hydropeaking retention basin, with a volume of about 300,000 m³. As the presented case studies are the largest measures for hydropeaking mitigation being currently implemented in Central Europe, they have pilot-character. Thus, ongoing research and monitoring programs are expected to improve the knowledge about hydropeaking mitigation.     

Biosynthesis of novel thermoplastic polythioesters by engineered Escherichia coli

The development of non-petrochemical sources for the plastics industry continues to progress as large multinationals focus on renewable resources to replace fossil carbon. Many bacteria are known to accumulate polyoxoesters as water-insoluble granules in the cytoplasm. The thermoplastic and/or elastomeric behaviour of these biodegradable polymers holds promise for the development of various technological applications. Here, we report the synthesis and characterization of microbial polythioesters (PTEs), a novel class of biopolymers of general technological relevance. Biosynthesis of PTE homopolymers was achieved using a recombinant strain of Escherichia coli that expressed a non-natural pathway consisting of a butyrate kinase, a phosphotransbutyrylase, and a PHA synthase. Different homopolymers were produced, consisting of either 3-mercaptopropionate, 3-mercaptobutyrate, or 3-mercaptovalerate repeating units, if the respective mercaptoalkanoic acids were provided as precursor substrates to the fermentative process. The PTEs contributed up to 30% (w/w) of the cellular dry weight and were identified as hydrophobic inclusions in the cytoplasm. The chemical and stereochemical homogeneity of the purified PTEs were identified by different methods, and the estimated physical properties were compared to the oxypolyester equivalents, revealing low crystalline order and, for the poly(3-mercaptopropionate) improved thermal stability. The ability to produce PTEs through a biosynthetic route opens up new avenues in the field of biomaterials.     

Correction for particle-wall interactions in the separation of colloids by flow field-flow fractionation

In the characterization of materials by field-flow fractionation (FFF), the experienced analyst understands the importance of incorporating additives in the carrier liquid that minimize or eliminate interactions between the analyte and accumulation wall, particularly in aqueous systems. However, as FFF is applied to more difficult samples, such as those with high surface energies, it is increasingly difficult to find additives that completely eliminate particle-wall interactions. Furthermore, the analyst may wish to use specific conditions that preserve the high surface energy of particles, to study their interaction with other materials through their behavior in the FFF channel. With this in mind, Williams and co-workers developed a model that quantifies the effect of particle-wall interactions in FFF using an empirically determined interaction parameter. In this work, the model is evaluated for the application of flow FFF in carrier liquids of low ionic strength, where particle-wall interactions are magnified. The retention of particles ranging in size from 64 to 1000 nm is measured using a wide range of field strengths and retention levels. The model is found to be generally valid over the entire range, except for minor discrepancies at lower levels of retention. Although retention levels are dramatically affected by particle-wall interactions, the point of steric inversion (500 nm), where the size-based elution order reverses, is not affected. When particle-wall interactions are not accounted for, they lead to a bias in particle sizes calculated from standard retention theory of up to 70%. The model can also be used to refine the measurement of channel thickness, which is important for the accurate conversion of retention parameters to particle sizes. In this work, for example, errors in channel thickness led to systematic errors on the order of 10% in particle diameter.     

Aspartame optical biosensor with bienzyme-immobilized eggshell membrane and oxygen-sensitive optode membrane

An aspartame optical biosensor has been fabricated by employing a bienzyme system composed of alpha-chymotrypsin and alcohol oxidase immobilized onto an eggshell membrane and an oxygen-sensitive optode membrane as the transducer. The detection schemes involve the enzymatic reactions of aspartame leading to the depletion of the oxygen level of the medium with a concomitant enhancement of the fluorescence intensity of the oxygen-sensitive membrane. The scanning electron and transmission electron micrographs show the microstructure of the eggshell membrane which is successfully immobilized with bienzyme. Using this novel immobilization technique, the aspartame biosensor shows extremely good stability with a shelf life of at least 8 months. The rate change of the fluorescence intensity in 4 min is found to be linearly related to the concentration of aspartame. The useful analytical working range of the biosensor is from 0.056 to 3.07 mM aspartame. The effects of temperature, pH, and ionic strength on the response of the aspartame biosensor are investigated in detail. Citric acid, cyclamic acid, D-fructose, D-galactose, D-glucose, hydrogen peroxide, DL-malic acid, L-phenylalanine, saccharin, sodium benzoate, and sucrose show no interferences but ethanol interferes strongly. The aspartame biosensor has been applied to determine aspartame contents in some commercial products.     

Measurements of chemical warfare agent degradation products using an electrophoresis microchip with contactless conductivity detector

This paper reports on a microfluidic device for the screening of organophosphonate nerve agent degradation products. The miniaturized system relies on an efficient chip-based separation of alkyl methylphosphonic acids (breakdown products of Sarin, Soman, and VX nerve agents) followed by their sensitive contactless conductivity detection. Experimental parameters relevant to the separation and detection processes have been optimized to yield high sensitivity (with 48-86 microg L(-1) detection limits), fast response (50 s for a three alkyl methylphosphonic acid mixture), high precision (RSD = 3.8-5.0%), and good linearity (over the 0.3-100 mg L(-1) range). Applicability to natural (river) water samples is demonstrated. The new microsystem offers promise for monitoring degradation products of chemical warfare agents, with advantages of speed/warning, efficiency, portability, sample size, and cost compared to conventional ion chromatography or capillary electrophoresis systems.     

4-Nitro-7-piperazino-2,1,3-benzoxadiazole as a reagent for monitoring of airborne isocyanates by liquid chromatography

4-Nitro-7-piperazino-2,1,3-benzoxadiazole (NBDPZ) is presented as a new reagent for the determination of mono- and diisocyanates in air samples. NBDPZ readily reacts with the airborne analytes, thus yielding the corresponding urea derivatives, which are subsequently separated by means of reversed-phase liquid chromatography. On a phenyl-modified stationary phase, excellent baseline separation for numerous mono- and diisocyanate derivatives is obtained. Both diode array and fluorescence detection are performed with limits of detection of 11-35 and 5-9 nmol/L for the individual derivatives, respectively. In contrast to established derivatizing agents for the analysis of isocyanates, NBDPZ provides for increased selectivity due to the favorable detection wavelengths in the visible range (UV/visible, absorption maximums approximately 480 nm; fluorescence, excitation maximums approximately 470 nm, emission maximums approximately 535 nm). In addition, the high molar absorptivities of the reagent and the derivatives provide excellent sensitivity that is superior to most literature-known methods. Finally, air sampling methods comprising both the use of impingers and test tubes are developed and successfully applied to the determination of isocyanates in gaseous samples. Excellent recovery reaching values of >90% is observed for each of the two techniques investigated.     

United States copyright law and digital sampling: Adding color to a grey area

This study evaluates the current application of United States copyright law in music sampling cases and used the Danger Mouse's Grey Album as a case in point. The following research questions structured the investigation: Are the current applications of United States copyright law appropriate for digital sampling in an international intellectual property environment? Is the practice of digital sampling stealing or recycling? How might the American legislature change copyright law to more clearly guide musical artists on the use of digital sampling? The research includes a review of sampling and its history, international and American copyright history, and the applicable elements of the Copyright Act of 1976, as well as interviews with legal professionals and musicians. The research suggests that the Copyright Act of 1976 is not adequate to address the technology and practices of digital sampling musicians, and that Congress needs to act precisely to amend the law and meet international legal standards.