Bone Marrow-Mesenchymal Stromal Cell Secretome as Conditioned Medium Relieves Experimental Skeletal Muscle Damage Induced by Ex Vivo Eccentric Contraction

Bone marrow-mesenchymal stem/stromal cells (MSCs) may offer promise for skeletal muscle repair/regeneration. Growing evidence suggests that the mechanisms underpinning the beneficial effects of such cells in muscle tissue reside in their ability to secrete bioactive molecules (secretome) with multiple actions. Hence, we examined the effects of MSC secretome as conditioned medium (MSC-CM) on ex vivo murine extensor digitorum longus muscle injured by forced eccentric contraction (EC). By combining morphological (light and confocal laser scanning microscopies) and electrophysiological analyses we demonstrated the capability of MSC-CM to attenuate EC-induced tissue structural damages and sarcolemnic functional properties’ modifications. MSC-CM was effective in protecting myofibers from apoptosis, as suggested by a reduced expression of pro-apoptotic markers, cytochrome c and activated caspase-3, along with an increase in the expression of pro-survival AKT factor. Notably, MSC-CM also reduced the EC-induced tissue redistribution and extension of telocytes/CD34⁺ stromal cells, distinctive cells proposed to play a “nursing” role for the muscle resident myogenic satellite cells (SCs), regarded as the main players of regeneration. Moreover, it affected SC functionality likely contributing to replenishment of the SC reservoir. This study provides the necessary groundwork for further investigation of the effects of MSC secretome in the setting of skeletal muscle injury and regenerative medicine.     

MMORPG Player actions: Network performance, session patterns and latency requirements analysis

Providing Massively Multiplayer Online Role-Playing Games (MMORPGs) is a big challenge for future mobile, IP-based networks. Understanding how the players’ actions affect the network parameters, the game platform, and the overall perceived quality is highly relevant for the purposes of game design, as well as for the networking infrastructure and network support for games. We break player actions down into discrete categories, and show that each category is distinct in terms of several key metrics. We discuss which categories of actions could be supported on current mobile devices, and present evidence in form of a user survey demonstrating the demand for such services. The starting points into the discussion include the networking, session and latency requirements for particular player actions on one side, and the players’ interest on the other. The Blizzard Entertainment’s World of Warcraft (WoW) is used as a case study.     

Microstructural study of aged ferrite powders for sensing layers

Nanosized powders of La_0.80Sr_0.20Fe_0.95Cu_0.05O_3?w were investigated in terms of structural, morphological, chemical and surface properties by using several characterization techniques. The XPS and IR measurements showed the presence of surface hydroxide and carbonates species. After calcination of the powders at 900 °C the amount of carbonates decreased but was still significant. The sensing activity of thick film based on La_0.80Sr_0.20Fe_0.95Cu_0.05O_3?w was tested as a function of relative humidity and the results indicate that (i) after one year of ambient storage the sensing material lost quite completely its sensitivity to humidity, (ii) the sensing activity of the film was mostly re-activated after a thermal treatment at 900 °C for 2 h, and (iii) the huge shift of the detection limit to low RH caused by the presence of 5 mol% Cu is also restored.     

Reforming of olive mill wastewater through a Pd-membrane reactor

This work investigates the exploitation of olive mill wastewater (OMW) for producing hydrogen in a membrane reformer.     

Infrastructures for the environment of multiagent systems

The notion of environment is receiving an increasing attention in the development of multiagent applications. This is witnessed by the emergence of a number of infrastructures providing agent designers with useful means to develop the agent environment, and thus to structure an effective multiagent application. In this paper we analyse the role and features of such infrastructures, and survey some relevant examples. We endorse a general viewpoint where the environment of a multiagent system is seen as a set of basic bricks we call environment abstractions, which (i) provide agents with services useful for achieving individual and social goals, and (ii) are supported by some underlying software infrastructure managing their creation and exploitation. Accordingly, we focus the survey on the opportunities that environment infrastructures provide to system designers when developing multiagent applications.     

Erosion behavior of EB-PVD 7YSZ coatings under corrosion/erosion regime: Effect of TBC microstructure and the CMAS chemistry

Aero-engines operating in dust-laden environments often encounter a lot of dust/sand that causes a severe problem to the TBCs by means of erosion. As the turbine entry temperatures are rising, molten sand is also a big concern to the life-time of TBCs.This paper deals with the TBC behavior under the combined influence of erosion and corrosion attack. Variations in TBC morphology, CMAS infiltration time and CMAS composition and their influence on the erosion resistance at room temperature were investigated. Two different EB-PVD 7YSZ morphologies consisting of a different porosity arrangement were tested in the erosion/corrosion regime. The more ‘Feathery’ structure has a better resistance to erosion compared to a more columnar ‘Normal’ structure, which leads to less degradation of the TBC. However, under the influence of CMAS infiltration the effect was found to be reversed. In general, CMAS-infiltrated EB-PVD TBCs exhibit a higher erosion resistance than the non-infiltrated ones.     

Crystalline Intermediate Phases in the Sol–Gel-Based Synthesis of La2NiO4+δ

X-ray diffraction and transmission electron microscopy were applied to investigate a sol–gel synthetic process for the mixed oxygen ion and electron conductor La₂NiO_4+δ with a K₂NiF₄ structure type. The development of the La₂NiO_4+δ is elucidated considering the influence of calcination temperatures and dwell times. Following the thermal decomposition of nitrate and organic precursors in an intermediate step, the lanthanum nickel oxide is obtained after a short dwell time above 750°C. This occurs by the transformation of an ultrafinely dispersed powder consisting of lanthanum oxycarbonate, lanthanum oxide, and nickel oxide. The pure La₂NiO_4+δ phase was obtained by similar solid-state reactions between nanocrystalline powder particles at just 950°C.     

A biochemical approach to adaptive service ecosystems

Emerging network scenarios call for innovative open service frameworks to ensure capability of self-adaptability and long-lasting evolvability. In this paper, we assess the need for such innovative service frameworks, and discuss how their engineering should get inspiration from natural ecosystems, i.e., by modelling services as autonomous individuals in an ecosystem of other services and data sources. We introduce a reference conceptual architecture with the goal of clarifying the concepts expressed and framing the several possible nature-inspired metaphors that could be adopted to realise the idea. On this basis, we go into details about one of such possible approaches, in which the rules governing the ecosystem are inspired by biochemical mechanisms. A case study is also introduced to exemplify the potentials of the presented biochemical approach and to experiment with some representative biochemistry-inspired patterns of adaptive service organisation and evolution.