Heat source identification and on-line temperature control by a Branch Eigenmodes Reduced Model

This paper presents the advantages of a Branch Eigenmodes Reduced Model used in a control process of a heating system.The experimental setup is a 3D heat conductive system in which a heat source is set. First, the reduced model is used to solve the non-linear Inverse Heat Conduction Problem: identification of the heat source strength variations from time-varying temperatures. Then, the reduced model is used to control hot points in the system. The objective of the method is to allow sequential temperature control by decreasing the computation time necessary for the simulation.     

Linear dimensionality reduction by maximizing the Chernoff distance in the transformed space

Linear dimensionality reduction (LDR) techniques are quite important in pattern recognition due to their linear time complexity and simplicity. In this paper, we present a novel LDR technique which, though linear, aims to maximize the Chernoff distance in the transformed space; thus, augmenting the class separability in such a space. We present the corresponding criterion, which is maximized via a gradient-based algorithm, and provide convergence and initialization proofs. We have performed a comprehensive performance analysis of our method combined with two well-known classifiers, linear and quadratic, on synthetic and real-life data, and compared it with other LDR techniques. The results on synthetic and standard real-life data sets show that the proposed criterion outperforms the latter when combined with both linear and quadratic classifiers.     

Fracture mechanics models applied to delayed failure of LVL beams

Several theories for modelling fracture and slow growth of a crack in wood have been developed. The various models may be differentiated by the specifically regarded stress levels, failure mechanisms and averaging procedures. This paper deals with the application of viscoelastic fracture mechanics models to predict delayed failure of a timber element in bending. Simulations are compared to experimental results of bending tests carried out on LVL (Laminated Veneer Lumber) notched beams. This analysis emphasizes the influence of the geometry and of the size of the beam as well as of the damage area on the delayed failure.     

Rheological properties of sMBR sludge under unsteady state conditions

The aim of this work is to investigate the rheological behaviour of MBR sludge for prolonged sludge retention time and during unsteady state conditions. The role of the total suspended solids and of the soluble organic matter is specifically studied through two running periods characterized by different organic loads and/or solid retention times. Diverse rheological parameters are examined. They are obtained from flow measurements carried out for an extent range of shear stress values.The obtained results show clearly that operating conditions capable of changing the total suspended solid (TSS) and soluble organic compound (COD) concentrations modify the rheological behaviour of the sludge.In comparison with the TSS concentration, the soluble organic compounds greatly affect the cohesion and the rigidity of the sludge three dimensional network. On the other hand, the COD concentration and the TSS content have equivalent quantitative influence on the rheological parameters characterizing the flow of the sludge.The results obtained are of great importance because operating conditions leading to the modification of the soluble microbial product concentration, and of the sludge rheological characteristics in the reactor, can strongly modify not only the mixing and aeration performances but also the membrane fouling of the MBR unit.     

A 1.4GS/s 9-bit 45 dB power control RFDAC for digital radio transmitters

This paper presents a high speed, 9-bit RF Digital-to-Analog Converter based on a new architecture implemented in a 0.13 μm BiCMOS process and able to adjust the output power by 45 dB to meet gain control requirements of the new communications standards with a SFDR >25 dBc. The maximum test-demonstrated frequency is 1.4 GHz and the chip dissipates <25 mW.     

Antimicrobial activity of nisin against Oenococcus oeni and other wine bacteria

Nisin is a bacteriocin used against food spoilage bacteria. Sulphur dioxide is a potent antioxidant as well as an antimicrobial agent widely used in the wine industry. In this study we describe the effect of these important antibacterial agents on the growth of a collection of 64 lactic acid bacteria (23 Oenococcus, 29 Lactobacillus, 3 Leuconostoc and 9 Pediococcus), 23 acetic acid bacteria and 20 yeast isolates, most of them recovered from wine. Minimal inhibitory concentrations (MIC) and minimal bactericide concentrations of nisin, potassium metabisulphite and ethanol were determined. Nisin MIC(50) values for the tested isolates were as follows: 0.024, 12.5, 200 and > or micro for oenococci, lactobacilli-pediococci-leuconostoc, acetic acid bacteria and yeasts, respectively. Synergistic effects on bacterial growth inhibition were observed, and potassium metabisulphite MIC(50) values decreased from one to three orders of dilution when it was combined with subinhibitory concentrations of nisin in the growth media. This effect was observed in all lactic acid bacteria species of our study. Significant differences in nisin sensitivity were observed between Gram-positive and Gram-negative bacteria, and between Oenococcus oeni and other species of lactic acid bacteria. It is concluded that appropriate combinations of nisin and metabisulphite could control the growth of spoilage bacteria in wine and therefore allow a decrease in the levels of sulphur dioxide currently used by the wine industry.     

SYBR®Green qPCR screening methods for the presence of “35S promoter” and “NOS terminator” elements in food and feed products

The Cauliflower Mosaic Virus “35S promotor” (p35S) and the Agrobacterium “Nopaline Synthase” terminator (tNOS) are the most represented generic recombinant elements in commercial genetically modified crops to date. A set of four new SYBR^®Green qPCR methods targeting the “p35S” and “tNOS” core elements have been developed. These qPCR methods generate short amplicons of 147 and 75 bp for the “p35S” element and 172 and 69 bp for the “tNOS” element. Single target plasmids containing these amplicons were constructed and allow determining the nominal melting temperature (T _m value) of each amplicon. The four methods are specific for their respective targets, and moreover, three of them are highly sensitive (up to 1–2 copies detectable) at a PCR efficiency ranging between 95 and 100%. The latter methods can detect their respective targets at 0.1% (w/w) gDNA levels and are suitable for detecting low levels of genetically modified materials containing the “p35S” and/or “tNOS” elements.     

TP53 Targeted Deep Sequencing of Cell-Free DNA in Esophageal Squamous Cell Carcinoma Using Low-Quality Serum: Concordance with Tumor Mutation

Circulating cell-free DNA (cfDNA) is emerging as a potential tumor biomarker. CfDNA-based biomarkers may be applicable in tumors without an available non-invasive screening method among at-risk populations. Esophageal squamous cell carcinoma (ESCC) and residents of the Asian cancer belt are examples of those malignancies and populations. Previous epidemiological studies using cfDNA have pointed to the need for high volumes of good quality plasma (i.e., >1 mL plasma with 0 or 1 cycles of freeze-thaw) rather than archival serum, which is often the main available source of cfDNA in retrospective studies. Here, we have investigated the concordance of TP53 mutations in tumor tissue and cfDNA extracted from archival serum left-over from 42 cases and 39 matched controls (age, gender, residence) in a high-risk area of Northern Iran (Golestan). Deep sequencing of TP53 coding regions was complemented with a specialized variant caller (Needlestack). Overall, 23% to 31% of mutations were concordantly detected in tumor and serum cfDNA (based on two false discovery rate thresholds). Concordance was positively correlated with high cfDNA concentration, smoking history (p-value = 0.02) and mutations with a high potential of neoantigen formation (OR; 95%CI = 1.9 (1.11–3.29)), suggesting that tumor DNA release in the bloodstream might reflect the effects of immune and inflammatory context on tumor cell turnover. We identified TP53 mutations in five controls, one of whom was subsequently diagnosed with ESCC. Overall, the results showed that cfDNA mutations can be reliably identified by deep sequencing of archival serum, with a rate of success comparable to plasma. Nonetheless, 70% non-identifiable mutations among cancer patients and 12% mutation detection in controls are the main challenges in applying cfDNA to detect tumor-related variants when blindly targeting whole coding regions of the TP53 gene in ESCC.     

Aquifex aeolicus membrane hydrogenase for hydrogen biooxidation: Role of lipids and physiological partners in enzyme stability and activity

Hydrogenase I from the hyperthermophilic bacterium Aquifex aeolicus is a good candidate for biotechnological devices thanks to its ability to oxidize hydrogen at high temperature, even in the presence of oxygen and CO. In order to enhance the enzyme stability and the catalytic efficiency, we investigated the hydrogen oxidation process with hydrogenase I embedded in a physiological-like environment. Hydrogenase I partners in the metabolic chain, namely membrane quinone and cytochrome b, were purified and fully characterized. The complex hydrogenase I–cytochrome b was inserted into liposomes. Surface Plasmon Resonance revealed that quinone took part in the stabilization of the complex. By use of molecular modelization and electrochemistry analysis, enzyme stability has been demonstrated to be stronger and enzymatic efficiency to be five times higher when hydrogenase is embedded into the liposomes. This result raises the possibility of using hydrogenases as biocatalysts in fuel cells.