Effect of chemical composition on the flocculation dynamics of latex-based synthetic activated sludge

This study investigates the effect of calcium, alginate, fibrous cellulose, and pH on the flocculation dynamics and final properties of synthetic activated sludges. A laboratory-scale batch reactor, fed with standard synthetic sludges was used. The effects of varying calcium concentration (5-25 mM), alginate concentration (25-125 mg/L), fibrous cellulose concentration (0.2-0.8 g/L) and pH (3-9) on the sludge characteristics were studied by varying one parameter whilst keeping the others constant. The results from experiments indicated that the calcium, alginate, fibrous cellulose, and pH had the critical effect on the aggregation rate, flocs size, and made the improvement of the final properties of sludge. Dynamic measurements have established the optimum conditions for floc formation and can accurately reflect the state of formation of the synthetic activated sludge flocs. These correlate well with measurements of settleability and turbidity of the synthetic activated sludge. The results of this study support the bonding theory and indicate that formation of cations-polymer complexes and polymer gelation are important means of flocculation. The development of synthetic activated sludges is suggested also to be a possible surrogate for studying the final properties of activated sludge.     

Entrapment of fluorescence signaling DNA enzymes in sol-gel-derived materials for metal ion sensing

Three fluorescence signaling DNA enzymes (deoxyribozymes or DNAzymes) were successfully immobilized within a series of sol-gel-derived matrixes and used for sensing of various metal ions. The DNAzymes are designed such that binding of appropriate metal ions induces the formation of a catalytic site that cleaves a ribonucleotide linkage within a DNA substrate. A fluorophore (fluorescein) and a quencher (DABCYL, [4-(4-dimethylaminophenylazo)benzoic acid]) were placed on the two deoxythymidines flanking the ribonucleotide to allow the generation of fluorescence upon the catalytic cleavage at the RNA linkage. In general, all DNAzymes retained at least partial catalytic function when entrapped in either hydrophilic or hydrophobic silica-based materials, but displayed slower response times and lower overall signal changes relative to solution. Interestingly, it was determined that maximum sensitivity toward metal ions was obtained when DNAzymes were entrapped into composite materials containing approximately 40% of methyltrimethoxysilane (MTMS) and approximately 60% tetramethoxysilane (TMOS). Highly polar materials derived from sodium silicate, diglycerylsilane, or TMOS had relatively low signal enhancements, while materials with very high levels of MTMS showed significant leaching and low signal enhancements. Entrapment into the hybrid silica material also reduced signal interferences that were related to metal-induced quenching; such interferences were a significant problem for solution-based assays and for polar materials. Extension of the solid-phase DNAzyme assay toward a multiplexed assay format for metal detection is demonstrated, and shows that sol-gel technology can provide new opportunities for the development of DNAzyme-based biosensors.     

Calibration of Speed Enforcement Down-The-Road Radars

We examine the measurement uncertainty associated with different methods of calibrating the ubiquitous down-the-road (DTR) radar used in speed enforcement. These calibration methods include the use of audio frequency sources, tuning forks, a fifth wheel attached to the rear of the vehicle with the radar unit, and the speedometer of the vehicle. We also provide an analysis showing the effect of calibration uncertainty on DTR-radar speed measurement uncertainty.     

Generalized scintillation detection and ranging results obtained by use of a modified inversion technique

The measurement of the strength of atmospheric optical turbulence by use of a modified generalized SCIDAR (scintillation detection and ranging) inversion technique is outlined and demonstrated. This new method for normalizing and inverting scintillation covariances incorporates the geometry specific to generalized SCIDAR. Examples of profiles from two astronomical observation sites are presented.     

Material Selection for Cryogenic Support Structures

Design specifications for the support structures of low temperature instrumentation often call for low thermal conductivity between temperature stages, high stiffness, and specific load bearing capabilities. While overall geometric design plays an important role in both overall stiffness and heat conduction between stages, material selection can affect a structure’s properties significantly. In this contribution, we suggest and compare several alternative materials to the current standard materials for building cryogenic support structures.     

Interactions between serotypes of dengue highlight epidemiological impact of cross-immunity

Dengue, a mosquito-borne virus of humans, infects over 50 million people annually. Infection with any of the four dengue serotypes induces protective immunity to that serotype, but does not confer long-term protection against infection by other serotypes. The immunological interactions between serotypes are of central importance in understanding epidemiological dynamics and anticipating the impact of dengue vaccines. We analysed a 38-year time series with 12 197 serotyped dengue infections from a hospital in Bangkok, Thailand. Using novel mechanistic models to represent different hypothesized immune interactions between serotypes, we found strong evidence that infection with dengue provides substantial short-term cross-protection against other serotypes (approx. 1–3 years). This is the first quantitative evidence that short-term cross-protection exists since human experimental infection studies performed in the 1950s. These findings will impact strategies for designing dengue vaccine studies, future multi-strain modelling efforts, and our understanding of evolutionary pressures in multi-strain disease systems.     

Predicting crack growth under thermo-mechanical cycling

Load-controlled tests were conducted on Inconel 718 under thermo-mechanical fatigue (TMF) to evaluate crack growth rate as a function of phase angle between load and temperature. A model is developed to predict TMF crack growth rates based solely on isothermal data. The model uses a linear summation concept based on contributions to crack growth of the two dominant mechanisms which are active at the minimum and maximum temperature of the cycle: mechanical fatigue and environmentally assisted crack growth. It was found that damage which contributes significantly to crack growth occurs only during the increasing load portion of a TMF cycle and only while the time-dependent contribution is an increasing function. A discussion of the dominant mechanisms and a comprehensive review of the literature in TMF crack growth is presented. Applicability of the modeling concepts to other materials is discussed.     

Friction stir welding for the transportation industries

This paper will focus on the relatively new joining technology—friction stir welding (FSW). Like all friction welding variants, the FSW process is carried out in the solid-phase. Generically solid-phase welding is one of the oldest forms of metallurgical joining processes known to man. Friction stir welding is a continuous hot shear autogenous process involving a non-consumable rotating probe of harder material than the substrate itself. In addition, FSW produces solid-phase, low distortion, good appearance welds at relatively low cost. Essentially, a portion of a specially shaped rotating tool is plunged between the abutting faces of the joint. Once entered into the weld, relative motion between the rotating tool and the substrate generates frictional heat that creates a plasticised region around the immersed portion of the tool. The contacting surface of the shouldered region of the tool and the workpiece top contacting surface also generates frictional heat. The shouldered region provides additional friction treatment to the weld region as well as preventing plasticised material being expelled. The tool is then translated with respect to the workpiece along the joint line, with the plasticised material coalescing behind the tool to form a solid-phase joint as the tool moves forward. Although the workpiece does heat up during FSW, the temperature does not reach the melting point. Friction stir welding can be used to join most aluminium alloys, and surface oxide presents no difficulty to the process. Trials undertaken up to the present time show that a number of light weight materials suitable for the automotive, rail, marine, and aerospace transportation industries can be fabricated by FSW.     

Tunable electronic interfaces between bulk semiconductors and ligand-stabilized nanoparticle assemblies

Interfaces between nanoscale and bulk electroactive materials are important for the design of electronic devices using solution-processed nanoparticles. We report that thin films of hexanethiolate-capped gold nanoparticles with a core diameter of 2.1+/-0.4 nm deposited onto n-InP wafers form Schottky contacts whose barrier height can be actively tuned from 0.27+/-0.03 to 1.11+/-0.09 eV by electrochemically adjusting the nanoparticle Fermi level. This result is remarkable because interfacial barriers at conventional metal-semiconductor contacts are largely insensitive to the initial Fermi level of the metal. Furthermore, it highlights two general features of solution-processed nanoparticle assemblies in comparison with traditional bulk electronic materials: (1) the ability of ions to permeate the nanoparticle assembly enables the electrochemical injection of charges and hence active control of the Fermi level, and (2) ligand passivation of nanoparticle surfaces prevents interfacial reactions with the semiconductor that could otherwise lead to strong Fermi-level pinning.     

Performance engineering - editorial

Imperial College London was host to the 24th Annual UK Performance Engineering Workshop in July 2008. UKPEW is an enjoyable workshop that brings together researchers in the performance engineering community to discuss quantitative aspects of, for instance, Grid computing, web and e-commerce, performance modelling techniques, power management and wireless network performance. In 2008, we had 29 papers presented over the two days of the workshop and this IET Software Special Issue represents significantly extended versions of the best selected papers from that workshop.