Optimization of adsorptive immobilization of alcohol dehydrogenases

In this work, a systematic examination of various parameters of adsorptive immobilization of alcohol dehydrogenases (ADHs) on solid support is performed and the impact of these parameters on immobilization efficiency is studied. Depending on the source of the enzymes, these parameters differently influence the immobilization efficiency, expressed in terms of residual activity and protein loading. Residual activity of 79% was achieved with ADH from bakers' yeast (YADH) after optimizing the immobilization parameters. A step-wise drying process has been found to be more effective than one-step drying. A hypothesis of deactivation through bubble nucleation during drying of the enzyme/glass bead suspension at low drying pressure (<45 kPa) is experimentally verified. In the case of ADH from Lactobacillus brevis (LBADH), >300% residual activity was found after drying. Hyperactivation of the enzyme is probably caused by structural changes in the enzyme molecule during the drying process. ADH from Thermoanaerobacter species (ADH T) is found to be stable under drying conditions (>15 kPa) in contrast to LBADH and YADH.     

Substance use by persons with recent spinal cord injuries.

Obtained substance use histories from 103 persons (aged 16–63 yrs) with recent spinal cord injuries (SCIs). Lifetime exposure to and recent use of several substances with abuse potential were greater than for a like-age national sample. Intoxication at time of injury was reported by 39%. The rate of exposure to and recent use of substances with abuse potential was significantly greater for Ss who reported intoxication at injury. Intoxication at SCI onset is a marker of preinjury substance use. It is important to screen for substance abuse in persons who incur traumatic injury. Timely assessment of and intervention for substance abuse could help enhance psychosocial and vocational outcomes. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A quantitative assessment of the hydrogen storage capacity of the UK continental shelf

Increased penetration of renewable energy sources and decarbonisation of the UK's gas supply will require large-scale energy storage. Using hydrogen as an energy storage vector, we estimate that 150 TWh of seasonal storage is required to replace seasonal variations in natural gas production. Large-scale storage is best suited to porous rock reservoirs. We present a method to quantify the hydrogen storage capacity of gas fields and saline aquifers using data previously used to assess CO₂ storage potential. We calculate a P50 value of 6900 TWh of working gas capacity in gas fields and 2200 TWh in saline aquifers on the UK continental shelf, assuming a cushion gas requirement of 50%. Sensitivity analysis reveals low temperature storage sites with sealing rocks that can withstand high pressures are ideal sites. Gas fields in the Southern North Sea could utilise existing infrastructure and large offshore wind developments to develop large-scale offshore hydrogen production.     

Developing an emotional distress item bank for cancer patients

Emotional distress is common among cancer patients during and after treatment. Many instruments have been used to measure emotional distress; however, none of them has emerged as a standard. Although the diversity of instruments has some merit, the lack of a common measure limits our ability to compare studies. This paper describes how we constructed a 46-item emotional distress bank. Using expert judgment, we selected a pool of items with emotional content from this six-instrument set. Rasch rating scale analysis helped us identify a set of general distress items with good model fit and a measurement gap causing floor effects. Additional items were written to augment the measure where found deficient. The resulting set of items reflects a spectrum of positive and negative affect. The measure demonstrated excellent reliability (person separation reliability = .96) and a wide range of emotional distress and was able to distinguish among levels of disease severity.     

A Mass-Preserving Two-Step Lagrange–Galerkin Scheme for Convection-Diffusion Problems

Journal of Scientific Computing - In this paper, we propose and analyze a mixed finite element scheme for stationary inductionless magnetohydrodynamic equations on a general Lipschitz domain. We...     

Start-ups and firm in-migration: evidence from the Swedish wholesale industry

We use a data set covering 13,471 Swedish limited liability firms in the Swedish wholesale industries during 2000–2004 to ascertain the determinants of new start-ups and of in-migration of firms. Access to a large harbor, international airport or large railroad classification yard in the municipality nearly triples the number of start-ups and increases the expected number of in-migrating firms with 53 %. The presence of a university, many educated workers and low local taxes are also associated with more start-ups and firm in-migration.     

Methyl Jasmonate-Induced Monoterpenes in Scots Pine and Norway Spruce Tissues Affect Pine Weevil Orientation

In large parts of Europe, insecticide-free measures for protecting conifer plants are desired to suppress damage by the pine weevil Hylobius abietis (L.). Treatment with methyl jasmonate (MeJA), a chemical elicitor already used in crop production, may enhance expression of chemical defenses in seedlings in conifer regenerations. However, in a previous experiment, MeJA treatment resulted in substantially better field protection for Scots pine (Pinus sylvestris L.) than for Norway spruce (Picea abies (L.) Karst.). Hypothesizing that the variations may be at least due partly to volatiles released by MeJA-treated seedlings and their effects on pine weevil orientation, we examined tissue extracts of seedlings (from the same batches as previously used) by two-dimensional GC-MS. We found that the MeJA treatment increased contents of the monoterpene (?)-β-pinene in phloem (the weevil’s main target tissue) of both tree species, however, the (?)-β-pinene/(?)-α-pinene ratio increased more in the phloem of P. sylvestris. We also tested the attractiveness of individual monoterpenes found in conifer tissues (needles and phloem) for pine weevils using an arena with traps baited with single-substance dispensers and pine twigs. Trap catches were reduced when the pine material was combined with a dispenser releasing (?)-β-pinene, (+)-3-carene, (?)-bornyl acetate or 1,8-cineole. However, (?)-α-pinene did not have this effect. Thus, the greater field protection of MeJA-treated P. sylvestris seedlings may be due to the selective induction of increases in contents of the deterrent (?)-β-pinene, in contrast to strong increases in both non-deterrent (?)-α-pinene and the deterrent (?)-β-pinene in P. abies seedlings.