Polyamine deactivation of integrated poly(dimethylsiloxane) structures investigated by radionuclide imaging and capillary electrophoresis experiments

The poly(dimethylsiloxane) (PDMS) material provides a number of advantageous features, such as flexibility, elasticity, and transparency, making it useful in integrated analytical systems. Hard fused-silica capillary structures and soft PDMS channels can easily be combined by a tight fit, which offers many alternatives for structure combinations. PDMS and fused silica are in different ways prone to adsorption of low levels of organic compounds. The need for modification of the inner wall surface of PDMS channels may often be necessary, and in this paper, we describe an easy and effective method using the amine-containing polymer PolyE-323 to deactivate both fused-silica and PDMS surfaces. The adsorption of selected peptides to untreated surfaces was compared to PolyE-323-modified surfaces, using both radionuclide imaging and capillary electrophoresis experiments. The polyamine modification displayed a substantially reduced adsorption of three hydrophobic test peptides compared to the native PDMS surface. Filling and storage of aqueous solution were also possible in PolyE-323-modified PDMS channels. In addition, hybrid microstructures of fused silica and PDMS could simultaneously be deactivated in one simple coating procedure.     

Multivalent HSA conjugates of 3'-sialyllactose are potent inhibitors of adenoviral cell attachment and infection

Adenoviruses of serotypes 8, 19 and 37 are the major cause of the severe eye infection EKC (epidemic keratoconjunctivitis). In general, all adenoviruses interact with their cellular receptors through the fibre proteins, which extend from the virus particle. Recently, adenovirus type 37 (Ad37) was found to bind and infect human corneal cells through attachment to carbohydrate structures that carry terminal alpha-(2-3)-linked sialic acids. Herein we present a synthetic route to a 3'-sialyllactose derivative and corresponding multivalent HSA conjugates with varying orders of valency. The potential of these compounds as inhibitors of EKC-causing adenovirus of serotype Ad37, was studied with both a binding assay and an infectivity assay. The results revealed that these compounds effectively prevent Ad37 from binding to and infecting human corneal epithelial (HCE) cells. Moreover, the inhibition is significantly increased with higher orders of multivalency.     

Cryostat for testing luminescence and radiation absorption in ionic crystals

A cryostat is described whose construction makes optical and X-ray examination of crysotals possible within the temperature range 70–500 K. The cryostat is equipped with two quartz glass windows, one metallized mylar window, and a sorption pump which allows an integral vacuum of the order of 10^?3 Pa to be maintained during measurements. A handle allows specimens to be turned by 180° so that an optimum position is obtained in relation to the window.     

Preface

For some time it has been recognized, at least implicitly, that catalysis and catalytic reaction engineering, both a mixture of art and science, enjoy a common synergistic basis, within which both the science and applied sciences (engineering) owe a debt to each other. While one might argue with respect to the relative merits of the contributions from one side of the issue or the other, we suggest that a respect for both aspects of this complex subject- the fundamentals (science) and their application (engineering), can only yield benefit to all concerned-the catalytic scientist and the catalytic reaction engineer.     

A Thermal Neutron Image Intensification System

An image intensifier tube of the vacuum tube type, sensitive to thermal neutrons has been developed and tested. The tube provides a bright visible output signal for an incoming thermal neutron image. Sensitivity, resolution, speed, contrast and relative response to radiation other than thermal neutrons all appear to be adequate for application to neutron imaging studies employing either reactor or non-reactor thermal neutron sources. The above characteristics, and applications of the system, are discussed.     

Tunable laser-diode-pumped Nd:YAG microcrystal lasers at 1.4 μm

TEM₀₀ laser operation of a monolithic Nd:YAG crystal laser has been achieved on three transitions at 1.414 μm, 1.444 μm and at 1.431 μm with laser diode pumping at 808 nm. The laser threshold was 1.5 W and the maximum output power 50 mW. The gain linewidths at 1.414 μm and 1.444 μm were determined by means of temperature tuning the microcrystal lasers. Calculations for designing tunable single frequency microcrystal lasers have been performed     

Attributions and disability acceptance following traumatic injury: A replication and extension.

(This reprinted article originally appeared in Journal of Personality and Social Psychology, 1977, Vol 35 [5], 351–363. The following abstract of the original article appeared in PA, Vol 62:3939.) Examined the relation between victims' attributions of causality for their accidents and their ability to cope with severe misfortune. A total of 29 individuals who had been paralyzed in serious accidents between the ages of 16 and 35 yrs were intensively interviewed. Individuals were selected who had been injured either 1–4 or 8–22 mo prior to the interview. Both quantitative and open-ended questions were used to elicit attributions of blame and causality by Ss; coping scores were obtained from a social worker and a nurse familiar with each case. Findings suggest that blaming another and feeling that one could have avoided the accident were successful predictors of poor coping; self-blame was a successful predictor of good coping. The question "Why me?" was posed by all Ss, and 28 of the 29 Ss offered to explain why the accident had happened to them. Their responses seemed to illustrate their need for meaning in explaining the selective incidence of the accident. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Gentle STEM: ADF imaging and EELS at low primary energies

Aberration correction of the scanning transmission electron microscope (STEM) has made it possible to reach probe sizes close to 1 Å at 60 keV, an operating energy that avoids direct knock-on damage in materials consisting of light atoms such as B, C, N and O. Although greatly reduced, some radiation damage is still present at this energy, and this limits the maximum usable electron dose. Elemental analysis by electron energy loss spectroscopy (EELS) is then usefully supplemented by annular dark field (ADF) imaging, for which the signal is larger. Because of its strong Z dependence, ADF allows the chemical identification of individual atoms, both heavy and light, and it can also record the atomic motion of individual heavy atoms in considerable detail. We illustrate these points by ADF images and EELS of nanotubes containing nanopods filled with single atoms of Er, and by ADF images of graphene with impurity atoms.     

Photo‐induced Charge Transfer and Relaxation of Persistent Charge Carriers in Polymer/Nanocrystal Composites for Applications in Hybrid Solar Cells

The photo‐induced charge transfer and the dynamics of persistent charge carriers in blends of semiconducting polymers and nanocrystals are investigated. Regioregular poly(3‐hexylthiophene) (P3HT) is used as the electron donor material, while the acceptor moiety is established by CdSe nanocrystals (nc‐CdSe) prepared via colloidal synthesis. As a reference system, organic blends of P3HT and [6,6]‐phenyl C₆₁‐butyric acid methyl ester (PCBM) are studied as well. The light‐induced charge transfer between P3HT and the acceptor materials is studied by photoluminescence (PL), photo‐induced absorption (PIA) and light‐induced electron spin resonance spectroscopy (LESR). Compared to neat P3HT samples, both systems show an intensified formation of polarons in the polymer upon photo‐excitation, pointing out successful separation of photogenerated charge carriers. Additionally, relaxation of the persistent charge carriers is investigated, and significant differences are found between the hybrid composite and the purely organic system. While relaxation, reflected in the transient signal decay of the polaron signal, is fast in the organic system, the hybrid blends exhibit long‐term persistence. The appearance of a second, slow recombination channel indicates the existence of deep trap states in the hybrid system, which leads to the capture of a large fraction of charge carriers. A change of polymer conformation due to the presence of nc‐CdSe is revealed by low temperature LESR measurements and microwave saturation techniques. The impact of the different recombination behavior on the photovoltaic efficiency of both systems is discussed.     

On Surface Losses in Direct Metal Laser Sintering Printed Millimeter and Submillimeter Waveguides

Different lengths of WR3 (220–330 GHz) and WR10 (75–110 GHz) waveguides are fabricated through direct metal laser sintering (DMLS). The losses in these waveguides are measured and modelled using the Huray surface roughness model. The losses in WR3 are around 0.3 dB/mm and in WR10 0.05 dB/mm. The Huray equation model is accounting relatively good for the attenuation in the WR10 waveguide but deviates more in the WR3 waveguide. The model is compared to finite element simulations of the losses assuming an approximate surface structure similar to the resulting one from the DMLS process.