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31.
    
Background: The aim of this feasibility study was to investigate the concentration level of CCL-20/MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin in the Peri-Implant Crevicular Fluid (PICF), from patients diagnosed with peri-implant mucositis and peri-implantitis, and to compare them with PICF from patients with healthy implants. Methods: Participants with at least one dental implant with healthy peri-implant tissues, peri-implant mucositis, or peri-implantitis were included. PICF was collected using paper strips from healthy and diseased peri-implant sites (n = 19). Biomarker levels were analyzed using a custom Multiplex ELISA Assay Kit. Results: In comparison to peri-implant health, the peri-implant mucositis group showed an increased concentration of CCL-20 MIP-3α, BAFF/BLyS, IL-23, RANKL, and Osteoprotegerin. The peri-implantitis group had the lowest median concentration of Osteoprotegerin (1963 ng/mL); this group had a similar concentration of RANKL (640.84 ng/mL) when compared to the peri-implant health group. BAFF/BLyS (17.06 ng/mL) showed the highest concentration in the peri-implantitis group. Conclusions: This feasibility study suggests that IL-23 and RANKL may help to elucidate the pathogenesis during the conversion from peri-implant health to peri-implantitis. Further research is required in BAFF/BLyS for the early diagnosis of peri-implantitis.  相似文献   
32.
    
The extensive application of herbicides in crop cultivation has indisputably led to the emergence of weed populations characterized by multiple herbicide resistance (MHR). This phenomenon is associated with the enhanced metabolism and detoxifying ability of endogenous enzymes, such as phi class glutathione transferases (GSTFs). In the present work, a library of mutant GSTFs was created by in vitro directed evolution via DNA shuffling. Selected gstf genes from the weeds Alopecurus myosuroides and Lolium rigidum, and the cereal crops Triticum durum and Hordeum vulgare were recombined to forge a library of novel chimeric GSTFs. The library was activity screened and the best-performing enzyme variants were purified and characterized. The work allowed the identification of enzyme variants that exhibit an eight-fold improvement in their catalytic efficiency, higher thermal stability (8.3 °C) and three-times higher inhibition sensitivity towards the herbicide butachlor. The crystal structures of the best-performing enzyme variants were determined by X-ray crystallography. Structural analysis allowed the identification of specific structural elements that are responsible for kcat regulation, thermal stability and inhibition potency. These improved novel enzymes hold the potential for utilization in biocatalysis and green biotechnology applications. The results of the present work contribute significantly to our knowledge of the structure and function of phi class plant GSTs and shed light on their involvement in the mechanisms of MHR.  相似文献   
33.
    
Syndecans act as independent co-receptors to exert biological activities and their altered function is associated with many pathophysiological conditions. Here, syndecan-1 and -4 were examined in lesional skin of patients with psoriasis. Immunohistochemical staining confirmed altered syndecan-1 distribution and revealed absence of syndecan-4 expression in the epidermis. Fibronectin (FN)—known to influence inflammation and keratinocyte hyperproliferation via α5β1 integrin in psoriasis—was also decreased. Syndecan-1 and -4 expression was analyzed in freshly isolated lesional psoriatic human keratinocytes (PHK) characterized based on their proliferation and differentiation properties. mRNA levels of syndecan-1 were similar between healthy and PHK, while syndecan-4 was significantly decreased. Cell growth and release of the pro-inflammatory Tumor Necrosis Factor-alpha (TNFα) were selectively and significantly induced in PHKs plated on FN. Results from co-culture of healthy keratinocytes and psoriatic fibroblasts led to the speculation that at least one factor released by fibroblasts down-regulate syndecan-1 expression in PHK plated on FN. To assay if biological treatments for psoriasis target keratinocyte proliferation, gelatin-based patches enriched with inteleukin (IL)-17α or TNFα blockers were prepared and tested using a full-thickness healthy epidermal model (Phenion®). Immunohistochemistry analysis showed that both blockers impacted the localisation of syndecan-1 within the refined epidermis. These results provide evidence that syndecans expression are modified in psoriasis, suggesting that they may represent markers of interest in this pathology.  相似文献   
34.
    
Fetal exposure in adverse environmental factors during intrauterine life can lead to various biological adjustments, affecting not only in utero development of the conceptus, but also its later metabolic and endocrine wellbeing. During human gestation, maternal bone turnover increases, as reflected by molecules involved in bone metabolism, such as vitamin D, osteocalcin, sclerostin, sRANKL, and osteoprotegerin; however, recent studies support their emerging role in endocrine functions and glucose homeostasis regulation. Herein, we sought to systematically review current knowledge on the effects of aforementioned maternal bone biomarkers during pregnancy on fetal intrauterine growth and metabolism, neonatal anthropometric measures at birth, as well as on future endocrine and metabolic wellbeing of the offspring. A growing body of literature converges on the view that maternal bone turnover is likely implicated in fetal growth, and at least to some extent, in neonatal and childhood body composition and metabolic wellbeing. Maternal sclerostin and sRANKL are positively linked with fetal abdominal circumference and subcutaneous fat deposition, contributing to greater birthweights. Vitamin D deficiency correlates with lower birthweights, while research is still needed on intrauterine fetal metabolism, as well as on vitamin D dosing supplementation during pregnancy, to diminish the risks of low birthweight or SGA neonates in high-risk populations.  相似文献   
35.
    
Universal Access in the Information Society - We present an investigation of the systematic differences in upper limb motor skills between children with learning disabilities (LDs) and children...  相似文献   
36.
    
Accumulating evidence suggests that the tumor microenvironment (TME) is involved in disease progression and drug resistance in B cell malignancies, by supporting tumor growth and facilitating the ability of malignant cells to avoid immune recognition. Immunomodulatory drugs (IMiDs) such as lenalidomide have some direct anti-tumor activity, but critically also target various cellular compartments of the TME including T cells, NK cells, and stromal cells, which interfere with pro-tumor signaling while activating anti-tumor immune responses. Lenalidomide has delivered favorable clinical outcomes as a single-agent, and in combination therapy leads to durable responses in chronic lymphocytic leukemia (CLL) and several non-Hodgkin lymphomas (NHLs) including follicular lymphoma (FL), diffuse large B cell lymphoma (DLBCL), and mantle cell lymphoma (MCL). Recently, avadomide, a next generation cereblon E3 ligase modulator (CELMoD), has shown potent anti-tumor and TME immunomodulatory effects, as well as promising clinical efficacy in DLBCL. This review describes how the pleiotropic effects of IMiDs and CELMoDs could make them excellent candidates for combination therapy in the immuno-oncology era—a concept supported by preclinical data, as well as the recent approval of lenalidomide in combination with rituximab for the treatment of relapsed/refractory (R/R) FL.  相似文献   
37.
This paper presents an analysis of current and potential cross-border congestion management methods for the EU electricity market. Many currently used techniques are reported and the main aspects of possible future are presented. The main steps of the newly established coordinated auction technique will be reported and an example of a real case for the South European Region will be implemented. Moreover, in order to eliminate loop flows, avoid congestions at the day ahead stage and increase the total transfer capacity of the south UCTE region (countries Greece, Albania and FYROM), it will be shown that Flexible Ac Transmission Systems technology can help market players to get access to more transfer capacity for their transactions, while security margins are being maintained.  相似文献   
38.
The conductivity and structure of 8-mol%-yttria-stabilized zirconia with additions of nickel oxide has been investigated, both in the as-prepared state and after reduction of NiO in hydrogen. During reduction at 1000°C, the conductivity decreases by 40%–50% and most of the NiO dissolved in the zirconia is exsolved in the form of nickel particles. This is accompanied by the formation of tetragonal zirconia precipitates of size 10–40 nm and by an increase in the lattice parameter. On reoxidation in air at 1000°C, the change in lattice parameter is partly reversed, but the conductivity shows no further changes.  相似文献   
39.
We use the T-matrix approach and the analytical orientation-averaging technique to formulate the problem of light scattering by an ensemble of rotationally symmetric particles in arbitrary orientation. The mathematical formulation yields analytical expressions for the elements of the ensemble-averaged scattering matrix that involve no more than four nested summations. An expansion into generalized spherical functions is used in the particular case where the scatterers are partially aligned along the direction of incidence. A computer code that implements the analytical expressions derived is publicly available on the World Wide Web at http://irctr.et.tudelft.nl/ -Skaropoulos/T-matrix.htm.  相似文献   
40.
The continuous increase of the computational power of programmable processors has established them as an attractive design alternative, for implementation of the most computationally intensive applications, like video compression. To enforce this trend, designers implementing applications on programmable platforms have to be provided with reliable and in-depth data and instruction analysis that will allow for the early selection of the most appropriate application for a given set of specifications. To address this need, we introduce a new methodology for early and accurate estimation of the number of instructions required for the execution of an application, together with the number of data memory transfers on a programmable processor. The high-level estimation is achieved by a series of mathematical formulas; these describe not only the arithmetic operations of an application, but also its control and addressing operations, if it is executed on a programmable core. The comparative study, which is done using three popular processors (ARM, MIPS, and Pentium), shows the high efficiency and accuracy of the methodology proposed, in terms of the number of executed (micro-)instructions (i.e. performance) and the number of data memory transfers (i.e. memory power consumption). Using the proposed methodology we estimated an average deviation of 23% in our estimated figures compared with the measurements taken from the real execution on the CPUs. This work was supported by the project PENED ’99 ED501 funded by GSRT of the Greek Ministry of Development, and the project PRENED ’99 KE 874 funded by the Research Committee of the Democritus University of Thrace. This work was partially sponsored by a scholarship from the Public Benefit Foundation of Alexander S. Onassis (Minas Dasygenis). Nikolaos Kroupis was born in Trikala in 1976. He receiver the engineering degree and Ms.C. degree in Department of Electrical and Computer Engineering from Democritous University of Thrace, Greece, in 2000 and 2002, respectively. Since 2002 he has been a Ph.D. student at the Laboratory of Electrical and Electronic Materials Technology. His research interests are in software/hardware co-design of embedded system for signal processing applications. Nikos D. Zervas received a Diploma in Electrical & Computer Engineering from University of Patras, Greece in 1997. He received the Ph.D. degree in the Department of Electrical and Computer Engineering of the same University in 2004. His research interests are in the area of high-level, power optimization techniques and methodologies for multimedia and telecommunication applications. He has received an award from IEEE Computer Society in the context of Low-Power Design Contest of 2000 IEEE Computer Elements Mesa Workshop. Mr. Zervas is a member of the IEEE, ACM and of the Technical Chamber of Greece. Minas Dasygenis was born in Thessaloniki in 1976. He received his Diploma in Electrical and Computer Engineering in 1999, from the Democritus University of Thrace, Greece, and for his diploma Thesis he was honored by The Technical Chamber of Greece and Ericsson Hellas. In 2005, he received his PhD Degree from the Democritus University of Thrace. His research interests include low-power VLSI design of arithmetic circuits, residue number system, embedded architectures, DSPs, hardware/ software codesign and IT security. He has published more than 20 papers in international journals and conferences and he has been a principal researcher in three European research projects. Konstantinos Tatas received his degree in Electrical and Computer Engineering from the Democritus University of Thrace, Greece in 1999. He received his Ph.D. in the VLSI Design and Testing Center in the same University by June 2005. He has been employed as an RTL designer in INTRACOM SA, Greece between 2000 and 2003. His research interests include low-power VLSI design of DSP and multimedia systems, computer arithmetic, IP core design and design for reuse. Antonios Argyriou received the degree in Electrical and Computer engineering from the Democritous University of Thrace, Greece, in 2001, and the M.S. and Ph.D. degrees in Electrical and Computer engineering from the Georgia Institute of Technology, Atlanta, in 2003 and 2005, respectively. His primary research interests include wireless networks, mobile computing and multimedia communications. He is a member of the IEEE and ACM. Dimitrios Soudris received his Diploma in Electrical Engineering from the University of Patras, Greece, in 1987. He received the Ph.D. Degree in Electrical Engineering, from the University of Patras in 1992. He is currently working as Ass. Professor in Dept. of Electrical and Computer Engineering, Democritus University of Thrace, Greece. His research interests include low power design, parallel architectures, embedded systems design, and VLSI signal processing. He has published more than 140 papers in international journals and conferences. He was leader and principal investigator in numerous research projects funded from the Greek Government and Industry as well as the European Commission (ESPRIT II-III-IV and 5th and 6th IST). He has served as General Chair and Program Chair for the International Workshop on Power and Timing Modelling, Optimisation, and Simulation (PATMOS). He received an award from INTEL and IBM for the project results of LPGD #25256 (ESPRIT IV). He is a member of the IEEE, the VLSI Systems and Applications Technical Committee of IEEE CAS and the ACM. Antonios Thanailakis was born in Greece on August 5, 1940. He received B.Sc. degrees in physics and electrical engineering from the University of Thessaloniki, Greece, 1964 and 1968, respectively, and the Msc. and Ph.D. Degrees in electrical engineering and electronics from UMIST, Manchester, U.K. in 1968 and 1971, respectively. He has been a Professor of Microelectronics in Dept. of Electrical and Computer Eng., Democritus Univ. of Thrace, Xanthi, Greece, since 1977. He has been active in electronic device and VLSI system design research since 1968. His current research activities include microelectronic devices and VLSI systems design. He has published a great number of scientific and technical papers, as well as five textbooks. He was leader for carrying out research and development projects funded by Greece, EU, or other organizations on various topics of Microlectronics and VLSI Systems Design (e.g. NATO, ESPRIT, ACTS, STRIDE).  相似文献   
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