Ionic liquid enables simple and rapid sample preparation of human culturing cells for scanning electron microscope analysis

Ionic liquid is a kind of salt that stays in a molten state even at room temperature. It does not vaporize at all in vacuum and facilitates electrical conductivity to the sample surfaces for observations with a scanning electron microscope (SEM). In this study, we used an ionic liquid in SEM for the first time to observe fixed human culture cells. The condition for the cell culture using wrapping sheets and SEM settings were varied to elucidate the optimized protocol. Compared to samples prepared by the conventional way, the ionic liquid‐treatment of samples gave SEM images of the cellular ultra structures in more detail, enabling observation of microvilli that made bridges between separated cells. In addition, the ionic liquid treatment is less time consuming as well as less laborious compared with the conventional way that includes dehydration, drying, and conductivity treatments. Totally, we concluded the ionic liquid is a useful reagent for SEM sample preparation. Microsc. Res. Tech., 2011. © 2010 Wiley‐Liss, Inc.     

Treatment capability of an up-flow anammox column reactor using polyethylene sponge strips as biomass carrier

The feasibility of applying a polyethylene (PE) sponge as a biomass carrier in an anaerobic ammonium oxidation (anammox) reactor and its nitrogen removal performance were also investigated. Experiments were carried out in an up-flow column reactor with synthetic inorganic wastewater. Experimental results indicate that reactor containing PE sponge biomass carriers showed a high nitrogen removal capability and exhibited stable performance. In addition, the reactor with 8 strips PE sponge as biomass carrier exhibited greater adaptation capacity compared to that with 6 strips and could achieve a high TN removal rate within a very short period. The ratio of NO₂-N removal and NO₃-N production to NH₄-N removal for the reactor was 1.26:0.21. Furthermore, to investigate the bacterial composition of the mature community, 16S rRNA sequences were amplified by PCR and analyses were conducted using DNA databases. Results showed that a new kind of anammox microorganism (Kumadai-1) was the dominant species in the reactor when using PE sponge as a biomass carrier.     

Morphological changes of Candida albicans induced by micafungin (FK463), a water-soluble echinocandin-like lipopeptide

Micafungin (FK463), a novel water-soluble echinocandin-like lipopeptide, exerted fungicidal action toward growing cells of Candida albicans at concentrations of 0.1 microg ml(-1) or above. The drug at these levels induced osmotically fragile cells and the resulting fungicidal effect was partially reversed when cultures were grown in the presence of an osmotic stabilizer. Candida cells incubated with fungicidal concentrations of micafungin gradually increased in size and/or became deformed. An electron microscopic study of such micafungin-treated cells revealed morphological alterations in the cell wall; deformation in contour, abnormal septum formation and decrease in thickness of the intermediate layer of the cell wall were prominent. In addition, the structure of cell membranes as well as of membranous cytoplasmic organelles was slightly impaired. These data suggest that micafungin principally affects the normal formation of the cell wall in growing Candida cells.     

Interactive segmentation of the cerebral lobes with fuzzy inference in 3T MR images.

Measurement of volume and surface area of the frontal, parietal, temporal and occipital lobes from magnetic resonance (MR) images shows promise as a method for use in diagnosis of dementia. This article presents a novel computer-aided system for automatically segmenting the cerebral lobes from 3T human brain MR images. Until now, the anatomical definition of cerebral lobes on the cerebral cortex is somewhat vague for use in automatic delineation of boundary lines, and there is no definition of cerebral lobes in the interior of the cerebrum. Therefore, we have developed a new method for defining cerebral lobes on the cerebral cortex and in the interior of the cerebrum. The proposed method determines the boundaries between the lobes by deforming initial surfaces. The initial surfaces are automatically determined based on user-given landmarks. They are smoothed and deformed so that the deforming boundaries run along the hourglass portion of the three-dimensional shape of the cerebrum with fuzzy rule-based active contour and surface models. The cerebrum is divided into the cerebral lobes according to the boundaries determined using this method. The reproducibility of our system with a given subject was assessed by examining the variability of volume and surface area in three healthy subjects, with measurements performed by three beginners and one expert user. The experimental results show that our system segments the cerebral lobes with high reproducibility.     

Long-wavelength vertical-cavity surface-emitting lasers on InP with lattice matched AlGaInAs-InP DBR grown by MOCVD

1.3- and 1.55-/spl mu/m vertical-cavity surface-emitting lasers (VCSELs) on InP have been realized. High-reflectivity AlGaInAs-InP lattice matched distributed Bragg reflectors (DBRs) were grown on InP substrates. 1.7 (for 1.3 /spl mu/m) and 2.0 (for 1.55 /spl mu/m) mW single mode power at 25/spl deg/C, 0.6 mW single mode power at 85/spl deg/C and lasing operation at >100/spl deg/C have been achieved. 10 Gbit/s error free transmissions through 10 km standard single mode fiber for 1.3-/spl mu/m VCSELs, and through 15 km nonzero dispersion shift fiber for 1.55-/spl mu/m VCSELs, have been demonstrated. With the addition of an SOA, 100 km error free transmission at 10 Gbit/s also has been demonstrated through a negative dispersion fiber. No degradation has been observed after over 2500-h aging test.     

Selective and in-situ determination of carbonate and oxide particles in aqueous solution using laser-induced breakdown spectroscopy (LIBS) for wearable information equipment

Laser-induced breakdown spectroscopy (LIBS) has been applied for quantitative analysis of Al₂O₃, CaCO₃ and MgO particles suspended in water. In the single elemental system, the plasma emission intensities of Al, Ca and Mg were linearly increased with concentration of elements in the range of 1.0×10^?5–1.0×10^?3  M, 1.0×10^?4–2.0×10^?3 M and 8.0×10^?5 –4.0×10^?3 M, respectively. We also investigated the concentration dependence of breakdown spectra for suspended mixtures of Al₂O₃, CaCO₃ and MgO particles. The emission lines, such as Al I, Ca I, Ca II and Mg I, were appeared in the LIBS spectrum simultaneously, and each emission peak could be deconvoluted. The plasma emission intensities of Al, Ca and Mg in the multielemental system were also linearly increased with their concentrations in the range of 1.0×10^?5–1.0×10^?3 M, 1.0×10^?4–2.0×10^?3 M and 4.0×10^?4–2.0×10^?3 M, respectively. LIBS was found to be available for quantitative and qualitative measurement of the concentrations of Al₂O₃, CaCO₃ and MgO particles suspended in water. The present results suggest that LIBS is a potentially useful tool for in-situ analysis on particles composition and concentrations for environmental monitoring by the wearable information equipments.     

In vivo performance of wax matrix granules prepared by a twin-screw compounding extruder

The in vivo performance of wax matrix granules (WMGs) prepared by a twin-screw compounding extruder was evaluated in fasted beagle dogs. In vitro dissolution behavior of the model drug, diclofenac sodium (DS), from WMGs was strongly influenced by pH in a dissolution medium due to its solubility (DS is soluble in pH 6.8 and insoluble in pH 1.2 and 4.0) and was independent of paddle rotation rate (50, 100, and 200 rpm) of the dissolution apparatus. Pharmacokinetics parameters such as mean residence time (MRT) showed a sustained action of WMGs in beagle dogs; however, the transit time of WMGs in the small intestine is found to control total drug absorption. Furthermore, the values of the area under the curve (AUC) of the plasma concentration-time curve and the maximum concentration Cmax significantly decreased with decreases in hydroxypropylcellulose (HPC) content in WMGs. Good correlation between one in vitro dissolution parameter (mean dissolution time, MDT) and two in vivo parameters (AUC12 and MRT) suggested that it would be possible to design WMGs with a desired in vivo performance by controlling HPC content.     

Survey of noise exposure level of national forestry workers

Eighty-one national forestry workers who were using chain saws, log cutters, log cutting machines, bush cleaners, timber-collecting cable machines and forklifts were examined for their level of noise exposure in a working day by using a portable sound meter. And their noise exposure levels Leq (8 h) for a year were estimated based on the measured noise levels and on the number of noise exposure days and hours in a year recorded in their work documents. The survey was made from July to December, 1988. The maximum noise levels with all the machines except a case of the forklift were above 100 dB, and with most chain saws they were above 110 dB. The amount of time that workers were exposed to the noise of logging and lumbering with chain saws, cutting by bush cleaners and timber-collecting cable machines without a cabin was longer than the allowable time for 90 dB and 95 dB. The number of noise exposure days in a year is fewer than reported in the past. The estimated Leq (8 h) for 32 out of 34 lumbermen surveyed was more than 85 dB, and for 5 lumbermen the Leq (8 h) was more than 90 dB. From these results, it can be concluded that there is a danger of noise induced hearing loss in national forestry workers using chain saws, log cutters, log cutting machines and timber-collecting cable machines without a cabin in 1987.     

Effects of N-methacryloyl amino acid applications on hybrid layer formation at the interface of intertubular dentin

To understand the role of NMAA in the bonding of composite resin to a dentin surface, we investigated the effects of N-methacryloyl amino acid (NMAA) application on the expansion of aggregated collagen fibers, formation of a hybrid layer, and the tensile bond strength between composite resin and dentin. Four NMAA derivatives--N-methacryloyl-alpha-glycine (NMGly), N-methacryloyl-gamma-amino n-butyric acid (NMBu), N-methacryloyl-alpha-hydroxyproline (NMHPro), and N-methacryloyl-alpha-glutamic acid (NMGlu)--were prepared and applied to dentin surfaces which had been etched with 40% by mass H3PO4 and air-blown. The shrunken collagenous layer expanded by approximately 50% to 70% by volume of the original collagenous layer thickness after application of the NMAA primers. Application of the bonding agent and composite resin after NMAA treatment resulted in the formation of a hybrid layer. The thickness of the hybrid layer was somewhat smaller than the collagenous layer formed by the NMAA treatment only, regardless of the type of NMAA used. The thickness of the hybrid layer was approximately ten times larger than that formed without NMAA treatment. Although all NMAA primers formed hybrid layers of similar thickness, higher tensile bond strengths, from 13 to 15 MPa, were obtained when etched and air-blown dentin was treated with NMBu, NMGly, or NMGlu. NMHPro gave only 6.6 MPa, a value similar to that obtained when no NMAA was used. We concluded, therefore, that formation of the hybrid layer is a necessary but insufficient condition for high bond strength.