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11.
Shukla Anshuman; Raje Manoj; Guptasarma Purnananda 《Protein engineering, design & selection : PEDS》2003,16(12):875-879
The backbone-reversed or retro, form of a modelall-ß-sheet protein, Escherichia coli CspA, was producedfrom a synthetic gene in E.coli in fusion with an N-terminalaffinity tag. Following purification under denaturing conditionsand dialysis-based removal of urea, the protein was found tofold into a soluble, poorly structured multimer. Upon concentration,this state readily transformed into amyloid nanofibres. CongoRed-binding amorphous forms were also observed. Since a ß-sheet-formingsequence is expected to retain high ß-sheet-formingpropensity even after backbone reversal and given the fact thatfolding of retro-CspA occurs only to a poorly structured form,we conclude that the increase effected in protein concentrationmay be responsible for the formation of intermolecular ß-sheets,facilitating the bleeding away of the proteins conformationalequilibrium into aggregates that generate well-formed fibres.Since every molecule in these fibres contains a peptide tagfor binding Ni2+, the fibres may provide a template for depositionof nickel to generate novel materials. Received April 1, 2003; revised October 27, 2003; accepted October 30, 2003 相似文献
12.
The gas-sensing mechanism of an n-type semiconductor (tin dioxide) gas sensor is reviewed in this paper. It is demonstrated
that very high sensitivity can be obtained only when the crystallite size is less than -10 nm. Various mechanisms involving
the surface and the bulk modifications of the semiconductor oxide gas sensors are discussed to improve the gas sensitivity.
Current challenges and problems in nanocrystalline oxide gas sensors are also presented. 相似文献
13.
Designing a distributed application is an extremely complex task. Proper facilities for prototyping distributed applications can be useful in evaluating a design, and also in understanding the effect of different parameters on the performance of an application. We describe a language for prototyping distributed applications, that supports different communication primitives with specified delay, and provides primitives to aid debugging and evaluation. Our environment for executing distributed programs supports heterogeneous computation in which processes can execute on different hardware. Different source languages can be used for coding different modules of the processes. The system has a centralized control and monitoring facility which is based on the Suntools window system. 相似文献
14.
H.P. Garg A.R. Shukla Indrajit Madhuri R.C. Agnihotri S. Chakravertty 《Applied Energy》1985,21(1):43-54
Indoor test methods for solar collectors are widely accepted and recommended by ASHRAE. The development of a solar simulator for indoor testing and the certification procedure for photo-thermal solar devices is discussed in this paper. The system consists of 14 quartz halogen lamps and provides a testing area of about 1 × 1 m. The irradiance can be varied from 400 to 1500 W/m2. This is achieved by varying the input power supplied to individual lamps. The radiation output characteristics of a single lamp have been studied. The distribution of intensity over the covered area of 1·20 × 1·20 m has been recorded using a precision Eppley pyranometer.A variety of collectors—evacuated tubular collectors and flat-plate collectors of both liquid and air heating types—has been tested under sufficiently stable insolation conditions. Results for a conventional solar air heater are presented together with suggestions for further improvements. 相似文献
15.
Fuel cell-based automobiles have gained attention in the last few years due to growing public concern about urban air pollution
and consequent environmental problems. From an analysis of the power and energy requirements of a modern car, it is estimated
that a base sustainable power ofca. 50 kW supplemented with short bursts up to 80 kW will suffice in most driving requirements. The energy demand depends greatly
on driving characteristics but under normal usage is expected to be 200 Wh/km. The advantages and disadvantages of candidate
fuel-cell systems and various fuels are considered together with the issue of whether the fuel should be converted directly
in the fuel cell or should be reformed to hydrogen onboard the vehicle. For fuel cell vehicles to compete successfully with
conventional internal-combustion engine vehicles, it appears that direct conversion fuel cells using probably hydrogen, but
possibly methanol, are the only realistic contenders for road transportation applications. Among the available fuel cell technologies,
polymer-electrolyte fuel cells directly fueled with hydrogen appear to be the best option for powering fuel cell vehicles
as there is every prospect that these will exceed the performance of the internal-combustion engine vehicles but for their
first cost. A target cost of $ 50/kW would be mandatory to make polymer-electrolyte fuel cells competitive with the internal
combustion engines and can only be achieved with design changes that would substantially reduce the quantity of materials
used. At present, prominent car manufacturers are deploying important research and development efforts to develop fuel cell
vehicles and are projecting to start production by 2005. 相似文献
16.
An experimental study is conducted to show the application of fiber optic sensors to fracture mechanics problems. Mode I stress intensity factors are obtained using single mode optical fibers in single edge notched specimens fabricated from aluminium. A Mach–Zehnder interferometric set up is used during the experiments. The experimental results compare well with theoretical predictions. 相似文献
17.
A female attending the skin outpatient department presented with a genital sore which was treated as chancroid. On further investigation it proved to be the opening of a sinus secondary to chronic osteomyelitis of the pubic symphysis. 相似文献
18.
Rahul Shukla Pier Luigi Dragotti Minh N Do Martin Vetterli 《IEEE transactions on image processing》2005,14(3):343-359
This paper presents novel coding algorithms based on tree-structured segmentation, which achieve the correct asymptotic rate-distortion (R-D) behavior for a simple class of signals, known as piecewise polynomials, by using an R-D based prune and join scheme. For the one-dimensional case, our scheme is based on binary-tree segmentation of the signal. This scheme approximates the signal segments using polynomial models and utilizes an R-D optimal bit allocation strategy among the different signal segments. The scheme further encodes similar neighbors jointly to achieve the correct exponentially decaying R-D behavior (D(R) - c(o)2(-c1R)), thus improving over classic wavelet schemes. We also prove that the computational complexity of the scheme is of O(N log N). We then show the extension of this scheme to the two-dimensional case using a quadtree. This quadtree-coding scheme also achieves an exponentially decaying R-D behavior, for the polygonal image model composed of a white polygon-shaped object against a uniform black background, with low computational cost of O(N log N). Again, the key is an R-D optimized prune and join strategy. Finally, we conclude with numerical results, which show that the proposed quadtree-coding scheme outperforms JPEG2000 by about 1 dB for real images, like cameraman, at low rates of around 0.15 bpp. 相似文献
19.
In this paper we present an efficient technique for piecewise cubic Bézier approximation of digitized curve. An adaptive breakpoint detection method divides a digital curve into a number of segments and each segment is approximated by a cubic Bézier curve so that the approximation error is minimized. Initial approximated Bézier control points for each of the segments are obtained by interpolation technique i.e. by the reverse recursion of De Castaljau's algorithm. Two methods, two-dimensional logarithmic search algorithm (TDLSA) and an evolutionary search algorithm (ESA), are introduced to find the best-fit Bézier control points from the approximate interpolated control points. ESA based refinement is proved to be better experimentally. Experimental results show that Bézier approximation of a digitized curve is much more accurate and uses less number of points compared to other approximation techniques. 相似文献
20.
Jain Shubhra Pattanaik K. K. Verma Rahul Kumar Shukla Anupam 《The Journal of supercomputing》2021,77(10):11458-11459
The Journal of Supercomputing - A correction to this paper has been published: https://doi.org/10.1007/s11227-021-03790-9 相似文献